Luciano Matias Matzkin

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Scholarly Contributions

Chapters

• Matzkin, L. M., & Markow, T. A. (2013). Transcriptional differentiation across the four cactus host races of Drosophila mojavensis. In Speciation: Natural Processes, Genetics and Biodiversity(pp 119-136). Nova Science Publishers Inc.
• Deckert-Cruz, D. J., Matzkin, L. M., Jr., J., & Rose, M. R. (2004). Electrophoretic analysis of methuselah flies from multiple species.. In Methuselah flies: A case study in the evolution of aging(pp 237-248). World Scientific Publishing Co.

Journals/Publications

• Benowitz, K. M., Allan, C. W., Jaworski, C. C., Sanderson, M. J., Diaz, F., Chen, X., & Matzkin, L. M. (2024). Fundamental patterns of structural evolution revealed by chromosome-length genomes of cactophilic Drosophila. Genome Research, 16(9). doi:doi:10.1093/gbe/evae191
• DuBose, J. G., Crook, T. B., Matzkin, L. M., & Haselkorn, T. S. (2024). The relative importance of host phylogeny and dietary convergence in shaping the bacterial communities hosted by several Sonoran Desert Drosophila species. Journal of evolutionary biology.
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  Complex eukaryotes vary greatly in the mode and extent that their evolutionary histories have been shaped by the microbial communities that they host. A general understanding of the evolutionary consequences of host-microbe symbioses requires that we understand the relative importance of host phylogenetic divergence and other ecological processes in shaping variation in host-associated microbial communities. To contribute to this understanding, we described the bacterial communities hosted by several Drosophila species native to the Sonoran Desert of North America. Our sampling consisted of four species that span multiple dietary shifts to cactophily, as well as the dietary generalist D. melanogaster, allowing us to partition the influences of host phylogeny and extant ecology. We found that bacterial communities were compositionally indistinguishable when considering incidence only but varied when considering the relative abundances of bacterial taxa. Variation in community composition was not explained by host phylogenetic divergence but could be partially explained by dietary variation. In support for an important role of diet as a source of ecological selection, we found that specialist cactophilic Drosophila deviated more from neutral predictions than dietary generalists. Overall, our findings provide insight into the evolutionary and ecological factors that shape host-associated microbial communities in a natural context.
• Goldberg, J. K., Allan, C. W., Copetti, D., Matzkin, L. M., & Bronstein, J. (2024). A pooled-sample draft genome assembly provides insights into host plant-specific transcriptional responses of a Solanaceae-specializing pest, (Hemiptera: Miridae). Ecology and evolution, 14(3), e10979.
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  The assembly of genomes from pooled samples of genetically heterogenous samples of conspecifics remains challenging. In this study, we show that high-quality genome assemblies can be produced from samples of multiple wild-caught individuals. We sequenced DNA extracted from a pooled sample of conspecific herbivorous insects (Hemiptera: Miridae: ) acquired from a greenhouse infestation in Tucson, Arizona (in the range of 30-100 individuals; 0.5 mL tissue by volume) using PacBio highly accurate long reads (HiFi). The initial assembly contained multiple haplotigs (>85% BUSCOs duplicated), but duplicate contigs could be easily purged to reveal a highly complete assembly (95.6% BUSCO, 4.4% duplicated) that is highly contiguous by short-read assembly standards (  = 675 kb; Largest contig = 4.3 Mb). We then used our assembly as the basis for a genome-guided differential expression study of host plant-specific transcriptional responses. We found thousands of genes ( = 4982) to be differentially expressed between our new data from individuals feeding on (Solanaceae) and existing RNA-seq data from (Solanaceae)-fed individuals. We identified many of these genes as previously documented detoxification genes such as glutathione-S-transferases, cytochrome P450s, and UDP-glucosyltransferases. Together our results show that long-read sequencing of pooled samples can provide a cost-effective genome assembly option for small insects and can provide insights into the genetic mechanisms underlying interactions between plants and herbivorous pests.
• Legan, A. W., Allan, C. W., Jensen, Z. N., Degain, B. A., Yang, F., Kerns, D. L., Benowitz, K. M., Fabrick, J. A., Li, X., Carrière, Y., Matzkin, L. M., & Tabashnik, B. E. (2024). Mismatch between lab-generated and field-evolved resistance to transgenic Bt crops in. Proceedings of the National Academy of Sciences of the United States of America, 121(47), e2416091121.
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  Transgenic crops producing crystalline (Cry) proteins from the bacterium (Bt) have been used extensively to control some major crop pests. However, many populations of the noctuid moth , one of the most important crop pests in the United States, have evolved practical resistance to several Cry proteins including Cry1Ac. Although mutations in single genes that confer resistance to Cry proteins have been identified in lab-selected and gene-edited strains of and other lepidopteran pests, the genetic basis of field-evolved resistance to Cry proteins in has remained elusive. We used a genomic approach to analyze the genetic basis of field-evolved resistance to Cry1Ac in 937 derived from 17 sites in seven states of the southern United States. We found evidence for extensive gene flow among all populations studied. Field-evolved resistance was not associated with mutations in 20 single candidate genes previously implicated in resistance or susceptibility to Cry proteins in or other lepidopterans. Instead, resistance in field samples was associated with increased copy number of a cluster of nine trypsin genes. However, trypsin gene amplification occurred in a susceptible sample and not in all resistant samples, implying that this amplification does not always confer resistance and mutations in other genes also contribute to field-evolved resistance to Cry1Ac in . The mismatch between lab-generated and field-evolved resistance in is unlike other cases of Bt resistance and reflects challenges for managing this pest.
• Matzkin, L. M., Bono, J. M., Pigage, H. K., Allan, C. W., Diaz, F., McCoy, J. R., Green, C. C., Callan, J. B., & Delahunt, S. P. (2024). Females translate male mRNA transferred during mating. iScience, 27(8), 110442.
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  Although RNA is found in the seminal fluid of diverse organisms, it is unknown whether it is functional within females. We developed a proteomic method (VESPA, Variant Enabled SILAC Proteomic Analysis) to test the hypothesis that male seminal fluid RNA is translated by females. We found 67 male-derived, female-translated proteins (mdFTPs) in female lower reproductive tracts, many with predicted functions relevant to reproduction. Knockout experiments indicate that mdFTPs play diverse roles in postmating interactions, affecting fertilization success, and the formation/persistence of the insemination reaction mass, a trait hypothesized to be involved in sexual conflict. These findings advance our understanding of reproduction by revealing a mechanism of postmating molecular interactions between the sexes that strengthens and extends male influences on reproduction in previously unrecognized ways. Given the diverse species that carry RNA in seminal fluid, this discovery has broad significance for understanding molecular mechanisms of cooperation and conflict during reproduction.
• Moreyra, N. N., Almeida, F. C., Allan, C., Frankel, N., Matzkin, L. M., & Hasson, E. (2023). Phylogenomics provides insights into the evolution of cactophily and host plant shifts in Drosophila. Molecular phylogenetics and evolution, 178, 107653.
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  Cactophilic species of the Drosophila buzzatii cluster (repleta group) comprise an excellent model group to investigate genomic changes underlying adaptation to extreme climate conditions and host plants. In particular, these species form a tractable system to study the transition from chemically simpler breeding sites (like prickly pears of the genus Opuntia) to chemically more complex hosts (columnar cacti). Here, we report four highly contiguous genome assemblies of three species of the buzzatii cluster. Based on this genomic data and inferred phylogenetic relationships, we identified candidate taxonomically restricted genes (TRGs) likely involved in the evolution of cactophily and cactus host specialization. Functional enrichment analyses of TRGs within the buzzatii cluster identified genes involved in detoxification, water preservation, immune system response, anatomical structure development, and morphogenesis. In contrast, processes that regulate responses to stress, as well as the metabolism of nitrogen compounds, transport, and secretion were found in the set of species that are columnar cacti dwellers. These findings are in line with the hypothesis that those genomic changes brought about key mechanisms underlying the adaptation of the buzzatii cluster species to arid regions in South America.
• Rodriguez, S. D., Allan, C. W., Duarte, S. D., Matzkin, L. M., Palumbo, J., & Carriere, Y. (2023). First Report of Tomato Spotted Wilt Virus infecting Lettuce in Yuma, Arizona. Plant disease.
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  Tomato spotted wilt virus (TSWV, family Tospoviridae, genus Orthotospovirus) is a thrips-vectored pathogen that infects lettuce (Lactuca sativa) and many vegetable crops (Kuo et al. 2014, Hasegawa et al. 2022). Another thrips-borne pathogen of lettuce, impatiens necrotic spot virus (INSV, Tospoviridae, Orthotospovirus), was first reported in 2021 in Yuma, Arizona (Hasegawa et al. 2022). Symptoms of both viruses in lettuce are similar and include necrotic spotting, leaf chlorosis and plant stunting (Kuo et al. 2014). Beginning February through April of 2022, lettuce displaying symptoms of orthotospovirus infection was collected from romaine lettuce (var. longifolia) fields in three regions of Yuma County. A total of 96 plants were collected (5 from Tacna on 2/21, 5 from Wellton on 2/21, 15 from Wellton on 3/23, 30 from Tacna on 4/4, 20 from Wellton on 4/4, and 21 from Yuma Valley on 4/4). The area of the fields ranged from 10 to 18 acres, and the percent disease incidence ranged from 0.8% (Tacna on 4/4) to 2.75% (Tacna on 2/21). Thrips vector were present in all fields were symptomatic plants were observed. One leaf disk per plant (8 mm in diameter) was sampled with a cork borer and grounded individually with a micro pestle in a 1.7 ml microcentrifuge tube with 150 ul of Tri-reagent (Molecular Research Center). Total RNA was extracted from each sample using the Zymo Direct-zol-96 kit (Zymo Research). Samples were diluted with water to a ratio of 1:10 after RNA extraction. RT-qPCR was performed in 20 ul reactions with 5 ul of input RNA using the PCR Biosystems qPCRBIO Probe 1-Step Go No-ROX for the cDNA/qPCR master mix. RT-qPCR assays were carried out in multiplex reactions using primers specific for TSWV and INSV, in addition to a lettuce internal control gene (LOC111918243), along with negative controls. Primer and probe sequence details are reported in supplemental Table 1. We used a cycle threshold (ct) < 40 to indicate a positive result for both INSV and TSWV (Chen et al. 2013; Boonham et al. 2002). RT-qPCR successfully amplified INSV in 90 out of 96 samples and TSWV in 8 out of 96 samples. These 8 samples tested positive for both TSWV and INSV, showing that INSV and TSWV co-infected lettuce plants. Thus overall, ∼ 95% of symptomatic plants were infected with INSV alone, and ∼ 8% were co-infected with TSWV and INSV. Amplicons of 4 samples testing positive for TSWV were sent for Sanger sequencing (Eurofins Genomics, Louisville, KY). All were identified as TSWV. One amplicon with TSWV was sequenced for INSV and double infection was confirmed. BLAST results from the NCBI nt database show 100% (138 bp) identity to TWSV (MW519211) for the 4 TWSV amplicons and 99.22% (137 bp) identity to INSV (KX790323) for the INSV amplicon. Sanger sequence data are in the GenBank (accession: OQ685940-OQ685944). Based on RT-qPCR results, all TSWV infected plants were also infected with INSV. INSV may have been introduced to Yuma by infected plants or thrips from lettuce transplants produced in California (Hasegawa et al. 2022). TSWV could have been introduced similarly. To our knowledge, this is the first report of TSWV infecting lettuce in Yuma and the first report of INSV and TSWV co-infecting lettuce. TSWV and INSV infections have remained low since their discovery in Yuma, in part due to effective cultural and chemical management by lettuce growers (Palumbo, 2022). However, an increase in disease incidence and severity in the future could have a significant negative impact on production of romaine lettuce in the region.
• Benowitz, K. M., Allan, C. W., Degain, B. A., Li, X., Fabrick, J. A., Tabashnik, B. E., Carrière, Y., & Matzkin, L. M. (2022). Novel genetic basis of resistance to Bt toxin Cry1Ac in Helicoverpa zea. Genetics, 221(1).
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  Crops genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringiensis have advanced pest management, but their benefits are diminished when pests evolve resistance. Elucidating the genetic basis of pest resistance to Bacillus thuringiensis toxins can improve resistance monitoring, resistance management, and the design of new insecticides. Here, we investigated the genetic basis of resistance to Bacillus thuringiensis toxin Cry1Ac in the lepidopteran Helicoverpa zea, one of the most damaging crop pests in the United States. To facilitate this research, we built the first chromosome-level genome assembly for this species, which has 31 chromosomes containing 375 Mb and 15,482 predicted proteins. Using a genome-wide association study, fine-scale mapping, and RNA-seq, we identified a 250-kb quantitative trait locus on chromosome 13 that was strongly associated with resistance in a strain of Helicoverpa zea that had been selected for resistance in the field and lab. The mutation in this quantitative trait locus contributed to but was not sufficient for resistance, which implies alleles in more than one gene contributed to resistance. This quantitative trait locus contains no genes with a previously reported role in resistance or susceptibility to Bacillus thuringiensis toxins. However, in resistant insects, this quantitative trait locus has a premature stop codon in a kinesin gene, which is a primary candidate as a mutation contributing to resistance. We found no changes in gene sequence or expression consistently associated with resistance for 11 genes previously implicated in lepidopteran resistance to Cry1Ac. Thus, the results reveal a novel and polygenic basis of resistance.
• Diaz, F., Allan, C. W., Chen, X., Coleman, J. M., Bono, J. M., & Matzkin, L. M. (2022). Divergent evolutionary trajectories shape the postmating transcriptional profiles of conspecific and heterospecifically mated cactophilic Drosophila females. Communications Biology, 5, 842. doi:doi.org/10.1038/s42003-022-03758-2
• Hurtado, J., Revale, S., & Matzkin, L. M. (2022). Propagation of seminal toxins through binary expression gene drives could suppress populations. Scientific reports, 12(1), 6332.
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  Gene drives can be highly effective in controlling a target population by disrupting a female fertility gene. To spread across a population, these drives require that disrupted alleles be largely recessive so as not to impose too high of a fitness penalty. We argue that this restriction may be relaxed by using a double gene drive design to spread a split binary expression system. One drive carries a dominant lethal/toxic effector alone and the other a transactivator factor, without which the effector will not act. Only after the drives reach sufficiently high frequencies would individuals have the chance to inherit both system components and the effector be expressed. We explore through mathematical modeling the potential of this design to spread dominant lethal/toxic alleles and suppress populations. We show that this system could be implemented to spread engineered seminal proteins designed to kill females, making it highly effective against polyandrous populations.
• Shaible, T. M., & Matzkin, L. M. (2022). Physiological and life history changes associated with seasonal adaptation in the cactophilic Drosophila mojavensis. Biology Open, 11(10), bio059610. doi:doi.org/10.1242/bio.059610
• Diaz, F., Allan, C. W., Markow, T. A., Bono, J. M., & Matzkin, L. M. (2021). Gene expression and alternative splicing dynamics are perturbed in head transcriptomes of heterospecifically mated females.. BMC Genomics, 35, 153-166. doi:10.1186/s12864-021-07669-0
• Diaz, F., Kuijper, A., Hoyle, R., Coleman, J. M., Talamantes, N., & Matzkin, L. M. (2021). Environmental predictability drives adaptive within- and transgenerational plasticity of heat tolerance across life stages and climatic regions. Functional Ecology, 35, 153-166. doi:10.1111/1365-2435.13704
• Diaz, F., Kuijper, B., Hoyle, R. B., Coleman, J. M., Talamantes, N., & Matzkin, L. M. (2021). Environmental predictability drives adaptive within- and transgenerational plasticity of heat tolerance across life stages and climatic regions. Functional Ecology, 35, 153-166. doi:doi:10.1111/1365-2435.13704
• Benowitz, K. M., Coleman, J. M., Allan, C. W., & Matzkin, L. M. (2020).

  Contributions of cis- and trans-Regulatory Evolution to Transcriptomic Divergence across Populations in the Drosophila mojavensis Larval Brain

  . Genome Biology and Evolution, 12(8), 1407-1418. doi:10.1093/gbe/evaa145
• Jaworski, C. C., Allan, C. W., & Matzkin, L. M. (2020). Chromosome-level hybrid de novo genome assemblies as an attainable option for non-model organisms. Molecular Ecology Resources, 20, 1277-1293. doi:10.1111/1755-0998.13176
• Khallaf, M. A., Auer, T. O., Grabe, V., Depetris-Chauvin, A., Ammagarahalli, B., Zhang, D. D., Lavista-Llanos, S., Kaftan, F., Weißflog, J., Matzkin, L. M., Rollmann, S. M., Löfstedt, C., Svatoš, A., Dweck, H. K., Sachse, S., Benton, R., Hansson, B. S., & Knaden, M. (2020). Mate discrimination among subspecies through a conserved olfactory pathway. Science advances, 6(25), eaba5279.
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  Communication mechanisms underlying the sexual isolation of species are poorly understood. Using four subspecies of as a model, we identify two behaviorally active, male-specific pheromones. One functions as a conserved male antiaphrodisiac in all subspecies and acts via gustation. The second induces female receptivity via olfaction exclusively in the two subspecies that produce it. Genetic analysis of the cognate receptor for the olfactory pheromone indicates an important role for this sensory pathway in promoting sexual isolation of subspecies, in combination with auditory signals. Unexpectedly, the peripheral sensory pathway detecting this pheromone is conserved molecularly, physiologically, and anatomically across subspecies. These observations imply that subspecies-specific behaviors arise from differential interpretation of the same peripheral cue, reminiscent of sexually conserved detection but dimorphic interpretation of male pheromones in . Our results reveal that, during incipient speciation, pheromone production, detection, and interpretation do not necessarily evolve in a coordinated manner.
• Allan, C. W., & Matzkin, L. M. (2019). Genomic analysis of the four ecologically distinct cactus host populations of Drosophila mojavensis. BMC Genomics. doi:https://doi.org/10.1186/s12864-019-6097-z
• Benowitz, K. M., Coleman, J. M., & Matzkin, L. M. (2019). Assessing the architecture of Drosophila mojavensis locomotor evolution with bulk segregant analysis.. G3.
• Coleman, J. M., Benowitz, K. M., Jost, A. G., & Matzkin, L. M. (2018). Behavioural evolution accompanying host shifts in cactophilic Drosophila larvae. Journal of Evolutionary Biology, 8, 6921-6931. doi:10.1002/ece3.4209
• Diaz-Gonzalez, F., Allan, C. W., & Matzkin, L. M. (2018). Adaptive amino acid evolution at odorant and gustatory receptors associated with host adaptation in cactophilic Drosophila. BMC Evolutionary Biology, 18, 144. doi:10.1186/s12862-018-1250-x
• Bono, J. M., Matzkin, L. M., Hoang, K., & Brandsmeier, L. (2015). Molecular evolution of candidate genes involved in post-mating-prezygotic reproductive isolation. Journal of Evolutionary Biology, 28(2), 403-14. doi:10.1111/jeb.12574
• Bono, J. M., Olesnicky, E. C., & Matzkin, L. M. (2015). Connecting genotypes, phenotypes and fitness: harnessing the power of CRISPR/Cas9 genome editing. Molecular Ecology, 24(15), 3810-3822. doi:10.1111/mec.13252
• Hoang, K., Matzkin, L. M., & Bono, J. M. (2015). Transcriptional variation associated with cactus host plant adaptation in Drosophila mettleri populations. Molecular Ecology, 24(20), 5186-5199. doi:10.1111/mec.13388
• Matzkin, L. M. (2014). Ecological Genomics of Host Shifts in Drosophila mojavensis. Advances in Experimental Medicine and Biology, 781(781), 233-247. doi:10.1007/978-94-007-7347-9_12
• Matzkin, L. M., Johnson, S., Paight, C., & Markow, T. A. (2013). Preadult Parental Diet Affects Offspring Development and Metabolism in Drosophila melanogaster. Plos One, 8(3), e59530. doi:10.1371/journal.pone.0059530
• Lang, M., Murat, S., Clark, A. G., Gouppil, G., Blais, C., Matzkin, L. M., Guittard, E., Yoshiyama-Yanagawa, T. .., Kataoka, H., Niwa, R., Lafont, R., Dauphin-Villemant, C. .., & Orgogozo, V. (2012). Mutations in the neverland Gene Turned Drosophila pachea into an Obligate Specialist Species. Science, 337(6102), 1658-1661.
• Matzkin, L. M. (2012). Population transcriptomics of cactus host shifts in Drosophila mojavensis. Molecular Ecology, 21(10), 2428-2439.
• Bono, J. M., Matzkin, L. M., Kelleher, E. S., & Markow, T. A. (2011). Postmating transcriptional changes in reproductive tracts of con- and heterospecifically mated Drosophila mojavensis females. Proceedings of the National Academy of Science, 108(19), 7878-7883.
• Matzkin, L. M., Johnson, S., Paight, C., Bozinovic, G., & Markow, T. A. (2011). Dietary Protein and Sugar Differentially Affect Development and Metabolic Pools in Ecologically Diverse Drosophila. Journal of Nutrition, 141(6), 1127-1133.
• Song, X., Goicoechea, J. L., Ammiraju, J., Luo, M., He, R., Lin, J., Lee, S., Sisneros, N., Watts, T., Kudrna, D. A., Golser, W., Ashley, E., Collura, K., Braidotti, M., Yu, Y., Matzkin, L. M., McAllister, B. F., Markow, T. A., & Wing, R. A. (2011). The 19 Genomes of Drosophila: A BAC Library Resource for Genus-Wide and Genome-Scale Comparative Evolutionary Research. Genetics, 187(4), 1023-1030.
• Markow, T. A., Beall, S., & Matzkin, L. M. (2009). Egg size, embryonic development time and ovoviviparity in Drosophila species. Journal of Evolutionary Biology, 22(2), 430-434.
• Matzkin, L. M., & Markow, T. A. (2009). Transcriptional regulation of metabolism associated with the increased desiccation resistance of the cactophilic Drosophila mojavensis. Genetics, 182(4), 1279-1288.
• Matzkin, L. M., Mutsaka, K., Johnson, S., & Markow, T. A. (2009). Metabolic pools differ among ecologically diverse Drosophila species. Journal of Insect Physiology, 55(12), 1145-1150.
• Matzkin, L. M., Watts, T. D., & Markow, T. A. (2009). Evolution of stress resistance in Drosophila: Interspecific variation in tolerance to desiccation and starvation. Functional Ecology, 23(3), 521-527.
• Pfeiler, E., Bitler, B. G., Castrezana, S., Matzkin, L. M., & Markow, T. A. (2009). Genetic diversification and demographic history of the cactophilic pseudoscorpion Dinocheirus arizonensis from the Sonoran Desert. Molecular Phylogenetics and Evolution, 52(1), 133-141.
• Bono, J. M., Matzkin, L. M., Castrezana, S., & Markow, T. A. (2008). Molecular evolution and population genetics of two Drosophila mettleri cytochrome P450 genes involved in host plant utilization. Molecular Ecology, 17(13), 3211-3221.
• Matzkin, L. M. (2008). The Molecular Basis of Host Adaptation in Cactophilic Drosophila: Molecular Evolution of a Glutathione S-Transferase Gene (GstD1) in Drosophila mojavensis. Genetics, 178(2), 1073-1083.
• Schaeffer, S. W., Bhutkar, A., McAllister, B. F., Matsuda, M., Matzkin, L. M., O'Grady, P. M., Rohde, C., Valente, V., Aguade, M., Anderson, W. W., Edwards, K., Garcia, A., Goodman, J., Hartigan, J., Kataoka, E., Lapoint, R. T., Lozovsky, E. R., Machado, C. A., Noor, M., , Papaceit, M., et al. (2008). Polytene Chromosomal Maps of 11 Drosophila Species: The Order of Genomic Scaffolds Inferred From Genetic and Physical Maps. Genetics, 179(3), 1601-1655.
• Clark, A. G., Eisen, M. B., Smith, D. R., Bergman, C. M., Oliver, B., Markow, T. A., Kaufman, T. C., Kellis, M., Gelbart, W., Iyer, V. N., Pollard, D. A., Sackton, T. B., Larracuente, A. M., Singh, N. D., Abad, J. P., Abt, D. N., Adryan, B., Aguade, M., Akashi, H., , Anderson, W. W., et al. (2007). Evolution of genes and genomes on the Drosophila phylogeny. Nature, 450(7167), 203-218.
• Flowers, J. M., Sezgin, E., Kumagai, S., Duvernell, D. D., Matzkin, L. M., Schmidt, P. S., & Eanes, W. F. (2007). Adaptive Evolution of Metabolic Pathways in Drosophila. Molecular Biology and Evolution, 24(6), 1347-1354.
• Machado, C. A., Matzkin, L. M., Reed, L. K., & Markow, T. A. (2007). Multilocus nuclear sequences reveal intra- and interspecific relationships among chromosomally polymorphic species of cactophilic Drosophila. Molecular Ecology, 16(14), 3009-3024.
• Matzkin, L. M., Watts, T. D., & Markow, T. A. (2007). Desiccation resistance in four Drosophila species: Sex and population effects.. Fly, 1(5), 268-273.
• Matzkin, L. M., Watts, T. D., Bitler, B. G., Machado, C. A., & Markow, T. A. (2006). Functional genomics of cactus host shifts in Drosophila mojavensis. Molecular Ecology, 15(14), 4635-4643.
• Matzkin, L. M. (2005). Activity variation in alcohol dehydrogenase paralogs is associated with adaptation to cactus host use in cactophilic Drosophila. Molecular Ecology, 14(7), 2223-2231.
• Matzkin, L. M., Merritt, T., Zhu, C., & Eanes, W. F. (2005). The Structure and Population Genetics of the Breakpoints Associated With the Cosmopolitan Chromosomal Inversion In(3R)Payne in Drosophila melanogaster. Genetics, 170(3), 1143-1152.
• Schmidt, P. S., Matzkin, L., Ippolito, M., & Eanes, W. F. (2005). Geographic variation in diapause incidence, life-history traits, and climatic adaptation in Drosophila melanogaster. Evolution, 59(8), 1721-1732.
• Matzkin, L. M. (2004). Population genetics and geographic variation of alcohol dehydrogenase (Adh) paralogs and glucose-6-phosphate dehydrogenase (G6pd) in Drosophila mojavensis. Molecular Biology and Evolution, 21(2), 276-285.
• Sezgin, E., Duvernell, D. D., Matzkin, L. M., Duan, Y., Zhu, C., Verrelli, B. C., & Eanes, W. F. (2004). Single-locus latitudinal clines and their relationship to temperate adaptation in metabolic genes and derived alleles in Drosophila melanogaster. Genetics, 168(2), 923-931.
• Gibbs, A. G., Fukuzato, F., & Matzkin, L. M. (2003). Evolution of water conservation mechanisms in Drosophila. Journal of Experimental Biology, 206(7), 1183-1192.
• Matzkin, L. M., & Eanes, W. F. (2003). Sequence variation of alcohol dehydrogenase (Adh) paralogs in cactophilic Drosophila. Genetics, 163(1), 181-194.
• Gibbs, A. G., & Matzkin, L. M. (2001). Evolution of water balance in the genus Drosophila. Journal of Experimental Biology, 204(13), 2331-2338.

Presentations

• Matzkin, L. M. (2021, December). Coevolutionary divergence and the origins reproductive incompatibilities. Department of Biological Sciences Seminar. Online (due to COVID): University of Alabama Huntsville.
• Benowitz, K., & Matzkin, L. M. (2019, June). Genome-wide mapping of multivariate complex trait evolution across Drosophila mojavensis populations. Society for the Study of Evolution Annual Meeting. Providence Rhode Island: Society for the Study of Evolution.
• Bono, J. M., Callan, J., Jordan, E., & Matzkin, L. M. (2019, June). Functional effects of Drosophila arizonae male reproductive genes on female fertility. Society for the Study of Evolution Annual Meeting. Providence Rhode Island: Society for the Study of Evolution.
• Diaz, F., Matzkin, L. M., Talamantes, N., Coleman, J. M., Hoyle, R., & Kuijper, A. (2019, June). Transcriptomic changes associated with the role of phenotypic plasticity and transgenerational effects in the thermal adaptation of desert Drosophila mojavensis. Society for the Study of Evolution Annual Meeting. Providence Rhode Island: Society for the Study of Evolution.
• Matzkin, L. M. (2019, April). Genomic, physiological and behavioral consequences of adaptation to local ecological conditions. Ecosystem Genomics Seminar. University of Arizona.
• Matzkin, L. M. (2019, August). A comparative genomic analysis of adaptation across cactophilic Drosophila species. PacBio Sequel II Arizona Genomics Institute Launch Event. University of Arizona: PacBio and AGI.
• Matzkin, L. M. (2019, March). Evolutionary consequences of adaptation to local ecological conditions. USDA ALARC - Seminar. Maricopa, Arizona: USDA ARS-Arid Land Agricultural Research Center.
• Matzkin, L. M., Allan, C. W., & Jaworski, C. (2019, November). A comparative genomic analysis of adaptation across cactophilic Drosophila species. Entomological Society of America Annual Meeting; Member Symposium: Preparing to Sequence the Planet - Starting with our Insect Friends. St. Louis, MO: Entomological Society of America.
• Matzkin, L. M., Diaz, F., & Bono, J. (2019, June). Transcriptional analysis of post-copulatory interactions in cactophilic Drosophila. Society for the Study of Evolution Annual Meeting. Providence Rhode Island: Society for the Study of Evolution.
• Matzkin, L. M. (2018, January). Genomics of ecological adaptation and speciation. AZ PopGroup. Tucson, Arizona.
• Jaworski, C., Allan, C., & Matzkin, L. M. (2017, December). Hybrid de novo genome assemblies as a valid option for non-model organisms: reduced cost but not quality. British Ecological Society Annual Meeting. Ghent, England.
• Matzkin, L. M. (2017, April). Evolution of reproductive barriers. Center of Insect Sciences Hexapodium. Tucson, Arizona.
• Matzkin, L. M. (2017, April). Genomics of ecological adaptation and reproductive incompatibility. Department of Ecology and Evolutionary Biology Seminar. Tucson, Arizona.
• Matzkin, L. M. (2017, November). Evolutionary genomics of adaptation and behavior in cactophilic Drosophila. Special Symposium, Genomics of adaptation: Linking the next generation of genome-wide analysis to understand and manage complex traits.. Denver, Colorado: Entomological Society of America Conference..

Poster Presentations

• Shaible, T. M., & Matzkin, L. M. (2019, June). Physiological Effects of Summer Temperature and Photoperiod in Drosophila mojavensis. Society for the Study of Evolution Annual Meeting. Providence Rhode Island: Society for the Study of Evolution.
• Benowitz, K., Coleman, J. M., & Matzkin, L. M. (2018, May). Genomics of larval locomotor evolution across Drosophila mojavensis populations. Population, Evolutionary and Quantitative Genetics Conference. Madison, Wisconsin: Genetics Society of America.
• Diaz, F., Coleman, J. M., Talamantes, N., & Matzkin, L. M. (2018, May). The relative importance of phenotypic plasticity, trans-generational effects and selection in heat shock responses of the cactophilic Drosophila mojavensis.. Population, Evolutionary and Quantitative Genetics Conference. Madison, Wisconsin: Genetics Society of America.
• Jaworski, C., Allan, C., & Matzkin, L. M. (2017, June). Identifying genome-scale structural differences between ecologically divergent populations of cactophilic flies. Society for the Study of Evolution Annual Conference. Portland, Oregon.