Mark E Van Dyke
- Associate Dean, Research
- Professor, Biomedical Engineering
- Member of the Graduate Faculty
Mark Van Dyke was born and raised in Michigan and graduated from Central Michigan University with a Bachelor of Science degree (chemistry, biology) in 1988. He began his professional career as an analytical chemist at the Dow Chemical Company in Midland, MI. As part of the Environmental Sciences Department, he served as a study director for research programs supporting US Environmental Protection Agency approval of new herbicides. In 1991, he moved to the Dow Corning Corporation and began work in toxicology, silicone biomaterials, and medical devices. After receiving the Dow Corning Fellowship, he attended graduate school at the University of Cincinnati in the Department of Chemical and Materials Engineering, earning his PhD in 1998. That same year, Dr. Van Dyke joined Southwest Research Institute (SwRI) in San Antonio, TX, the largest independent non-profit research and development lab in the US. During his tenure with SwRI, Dr. Van Dyke was a principal investigator and study director for several large biomaterial development programs. His primary area of interest was in the development of naturally-derived biomaterials and their application to wound healing and tissue engineering. In 2004, Dr. Van Dyke joined the faculty of the Wake Forest University School of Medicine where he expanded his investigations into the use of keratin biomaterials for regenerative medicine applications. In 2012 he joined the faculty of Virginia Tech in the Department of Biomedical Engineering and Mechanics where his research included investigation of the solution behavior and self-assembly of keratin nanomaterials and their development into products for medical devices, tissue engineering, drug and cell delivery, and trauma applications. In 2020, Dr. Van Dyke joined the University of Arizona as the Associate Dean of Research in the College of Engineering, and a Tenured Professor in the Department of Biomedical Engineering. Dr. Van Dyke has published more than 80 papers and book chapters, is an inventor or co-inventor on 34 issued US patents and more than 80 US and international patents pending, many related to keratin biomaterials and their application to tissue engineering and trauma, and a co-founder of three startup companies. His teaching interests include regenerative medicine, biomaterials and healthcare entrepreneurship.
- Ph.D. Materials Science
- University of Cincinnati, Cincinnati, Ohio, United States
- Synthesis and Characterization of Silicon-Containing Monomers, Polymers and Copolymers
- B.S. Chemistry
- Central Michigan University, Mt. Pleasant, Michigan, United States
- University of Arizona, Tucson, Arizona (2020 - Ongoing)
- University of Arizona, Tucson, Arizona (2020 - Ongoing)
- Virginia Polytechnic Institute and State University (2012 - 2020)
- KeraNetics LLC (2008 - 2012)
- Wake Forest School of Medicine (2004 - 2012)
- Southwest Research Institute (1998 - 2004)
- Dow Corning Corporation (1992 - 1994)
- The Dow Chemical Company (1988 - 1991)
Regenerative Medicine;Tissue Engineering;Biomaterials;Medical Devices;Healthcare Regulation;Intellectual Property Strategy;Entrepreneurship
Biomaterials;Medical Devices;Prosthetics;Regenerative Medicine;Tissue Engineering;Entrepreneurial Ecosystems;
Special Topics in EntrepENTR 296 (Fall 2023)
Tech VenturesENTR 400 (Spring 2023)
- Asthana, A., Tamburrini, R., Chaimov, D., Gazia, C., Walker, S. J., Van Dyke, M., Tomei, A., Lablanche, S., Robertson, J., Opara, E. C., Soker, S., & Orlando, G. (2021). Comprehensive characterization of the human pancreatic proteome for bioengineering applications. Biomaterials, 270, January. doi:10.1016/j.biomaterials.2020.120613More infoInteractions between the pancreatic extracellular matrix (ECM) and islet cells are known to regulate multiple aspects of islet physiology, including survival, proliferation, and glucose-stimulated insulin secretion. Recognizing the essential role of ECM in islet survival and function, various engineering approaches have been developed that aim to utilize ECM-based materials to recreate a native-like microenvironment. However, a major impediment to the success of these approaches has been the lack of a robust and comprehensive characterization of the human pancreatic proteome. Herein, by combining mass spectrometry (MS) and multiplex ELISA, we have provided an improved workflow for the in-depth profiling of the proteome, including minor constituents that are generally underrepresented. Moreover, we have further validated the effectiveness of our detergent-free decellularization protocol in the removal of cellular proteins and retention of the matrisome. It has also been established that the decellularized ECM and its derivatives can provide more tissue-specific cues than traditionally used biological scaffolds and are therefore more physiologically relevant for the development of hydrogels, bioinks and medium additives, in order to create a pancreatic niche. The data generated in this study would contribute significantly to the efforts of comprehensively defining the ECM atlas and also serve as a standard for the human pancreatic proteome to provide further guidance for design and engineering strategies for improved tissue engineering scaffolds.
- Parker, R. N., Alexis, T., Stefaniak, K. L., Van Dyke, M. E., & Grove, T. Z. (2020). A comparative study of materials assembled from recombinant K31 and K81 and extracted human hair keratins. Biomedical Materials, 15, 065006. doi:10.1088/1748-605X/ab98e8More infoNatural biopolymers have found success in tissue engineering and regenerative medicine applications. Their intrinsic biocompatibility and biological activity make them well suited for biomaterials development. Specifically, keratin-based biomaterials have demonstrated utility in regenerative medicine applications including bone regeneration, wound healing, and nerve regeneration. However, studies of structure-function relationships in keratin biomaterials have been hindered by the lack of homogeneous preparations of materials extracted and isolated from natural sources such as wool and hair fibers. Here we present a side-by-side comparison of natural and recombinant human hair keratin proteins K31 and K81. When combined, the recombinant proteins (i.e. rhK31 and rhK81) assemble into characteristic intermediate filament-like fibers. Coatings made from natural and recombinant dimers were compared side-by-side and investigated for coating characteristics and cell adhesion. In comparison to control substrates, the recombinant keratin materials show a higher propensity for inducing involucrin and hence, maturation in terms of potential skin cell differentiation.
- Thompson, M., Giuffre, A., McClenny, C., & Van Dyke, M. E. (2021). A keratin-based microparticle for cell delivery. Journal of Biomaterial Applications, 35(6), 579-91. doi:10.1177/0885328220951892More infoKeratin-based biomaterials represent an attractive opportunity in the fields of wound healing and tissue regeneration, not only for their chemical and physical properties, but also for their ability to act as a delivery system for a variety of payloads. Importantly, keratins are the only natural biomaterial that is not targeted by specific tissue turnover-related enzymes, giving it potential stability advantages and greater control over degradation after implantation. However, in-situ polymerization chemistry in some keratin systems are not compatible with cells, and incorporation within constructs such as hydrogels may lead to hypoxia and cell death. To address these challenges, we envisioned a pre-formed keratin microparticle on which cells could be seeded, while other payloads (e.g. drugs, growth factors or other biologic compounds) could be contained within, although studies investigating the potential partitioning between phases during emulsion polymerization would need to be conducted. This study employs well-established water-in-oil emulsion procedures as well as a suspension culture method to load keratin-based microparticles with bone marrow-derived mesenchymal stem cells. Fabricated microparticles were characterized for size, porosity and surface structure and further analyzed to investigate their ability to form gels upon hydration. The suspension culture technique was validated based on the ability for loaded cells to maintain their viability and express actin and vinculin proteins, which are key indicators of cell attachment and growth. Maintenance of expression of markers associated with cell plasticity was also investigated. As a comparative model, a collagen-coated microparticle (Sigma) of similar size was used. Results showed that an oxidized form of keratin (“keratose” or “KOS”) formed unique microparticle structures of various size that appeared to contain a fibrous sub-structure. Cell adhesion and viability was greater on keratin microparticles compared to collagen-coated microparticles, while marker expression was retained on both.