Rocio Zapata Bustos
- Research Assistant Professor, Medicine
Dr. Zapata is a Research Assistant Professor in the College of Medicine at University of Arizona since November 2018. She joined the University of Arizona in May 2016 as a postdoctoral fellow. Prior to joining the University of Arizona, Dr. Zapata was a postdoctoral fellow in the Center for Metabolic and Vascular Biology (CMVB) at the Arizona State University (ASU) and Mayo Clinic at Scottsdale, Arizona. Dr. Zapata completed her master and Ph.D. degrees at the Instituto Potosino de Investigación Ciencia y Tecnología (IPICYT) in San Luis Potosi, Mexico.
Dr. Zapata works under the guidance and mentorship of Dr. Lawrence Mandarino and oversees the activities of the Clinical Studies Team housed in the Clinical & Translational Sciences Research Center (CATS).
- Ph.D. Molecular Biology
- Instituto Potosino de Investigacion Cientifica y Tecnologica (IPICYT), San Luis Potosi, San Luis Potosi, Mexico
- “Antidiabetic molecular mechanisms of plants used in the traditional medicine”
- M.S. Molecular Biology
- Instituto Potosino de Investigación Científica y Tecnológica (IPICYT), San Luis Potosi, San Luis Potosi, Mexico
- “Molecular mechanisms of the hypoglycemic effect of plants traditionally used as antidiabetics”
- B.S. Pharmaco-biological chemistry
- Autonomous University of San Luis Potosí, UASLP, San Luis Potosi, San Luis Potosi, Mexico
- “Preadipocyte cellular proliferation and differentiation in L15 medium”
- University of Arizona, Tucson, Arizona (2016 - 2019)
- Mayo/ASU Center for Metabolic and Vascular Biology (2014 - 2016)
- Award to 2nd place in poster presentation
- Arizona Physiological Society., Fall 2017
- Doctoral Fellowship
- CONACYT (Consejo Nacional de Ciencia y Tecnología, National Council of Science and Technology), Fall 2009
- Award “Alfredo Sánchez Marroquín 2009” in the Undergraduate category for “Senior Thesis”.
- Mexican Society of Biotechnology and Bioengineering., Summer 2009
- Award “Dr. Miguel Otero y Arce 2009” in Basic Research category.
- Ministry of Health of San Luis Potosí., Spring 2009
- Honorable Mention at the “First National Contest in Basic Sciences and Creativity”
- Universidad Iberoamericana, Summer 2008 (Award Nominee)
- Award “Dr. Miguel Otero y Arce 2008” First place in Basic Research category.
- Ministry of Health of San Luis Potosí., Spring 2008
- Masters Fellowship
- CONACYT (Consejo Nacional de Ciencia y Tecnología, National Council of Science and Technology), Fall 2007
Licensure & Certification
- Bachelor in Science, Autonomous University of San Luis Potosí, UASLP (2008)
Dr. Zapata’s research interest are to study the molecular mechanisms underlying mitochondrial dysfunction and the role of mitochondria in insulin resistance and metabolic inflexibility; as well as understanding the molecular mechanisms regulating exercise response and exercise resistance in patients with type 2 diabetes mellitus.
No activities entered.
- Willis, W. T., Miranda-Grandjean, D., Hudgens, J., Willis, E. A., Finlayson, J., De Filippis, E. A., Zapata Bustos, R., Langlais, P. R., Mielke, C., & Mandarino, L. J. (2018). Dominant and sensitive control of oxidative flux by the ATP-ADP carrier in human skeletal muscle mitochondria: Effect of lysine acetylation. Archives of biochemistry and biophysics, 647, 93-103.More infoThe adenine nucleotide translocase (ANT) of the mitochondrial inner membrane exchanges ADP for ATP. Mitochondria were isolated from human vastus lateralis muscle (n = 9). Carboxyatractyloside titration of O consumption rate (J) at clamped [ADP] of 21 μM gave ANT abundance of 0.97 ± 0.14 nmol ANT/mg and a flux control coefficient of 82% ± 6%. Flux control fell to 1% ± 1% at saturating (2 mM) [ADP]. The KmADP for J was 32.4 ± 1.8 μM. In terms of the free (-3) ADP anion this KmADP was 12.0 ± 0.7 μM. A novel luciferase-based assay for ATP production gave KmADP of 13.1 ± 1.9 μM in the absence of ATP competition. The free anion KmADP in this case was 2.0 ± 0.3 μM. Targeted proteomic analyses showed significant acetylation of ANT Lysine23 and that ANT1 was the most abundant isoform. Acetylation of Lysine23 correlated positively with KmADP, r = 0.74, P = 0.022. The findings underscore the central role played by ANT in the control of oxidative phosphorylation, particularly at the energy phosphate levels associated with low ATP demand. As predicted by molecular dynamic modeling, ANT Lysine23 acetylation decreased the apparent affinity of ADP for ANT binding.
- Luo, M., Mengos, A. E., Ma, W., Finlayson, J., Bustos, R. Z., Xiao Zhu, Y., Shi, C. X., Stubblefield, T. M., Willis, W. T., & Mandarino, L. J. (2017). Characterization of the novel protein KIAA0564 (Von Willebrand Domain-containing Protein 8). Biochemical and biophysical research communications, 487(3), 545-551.More infoThe VWA8 gene was first identified by the Kazusa cDNA project and named KIAA0564. Based on the observation, by similarity, that the protein encoded by KIAA0564 contains a Von Willebrand Factor 8 domain, KIAA0564 was named Von Willebrand Domain-containing Protein 8 (VWA8). The function of VWA8 protein is almost unknown. The purpose of this study was to characterize the tissue distribution, cellular location, and function of VWA8. In mice VWA8 protein was mostly distributed in liver, kidney, heart, pancreas and skeletal muscle, and is present as a long isoform and a shorter splice variant (VWA8a and VWA8b). VWA8 protein and mRNA were elevated in mouse liver in response to high fat feeding. Sequence analysis suggests that VWA8 has a mitochondrial targeting sequence and domains responsible for ATPase activity. VWA8 protein was targeted exclusively to mitochondria in mouse AML12 liver cells, and this was prevented by deletion of the targeting sequence. Moreover, the VWA8 short isoform overexpressed in insect cells using a baculovirus construct had in vitro ATPase activity. Deletion of the Walker A motif or Walker B motif in VWA8 mostly blocked ATPase activity, suggesting Walker A motif or Walker B motif are essential to the ATPase activity of VWA8. Finally, homology modeling suggested that VWA8 may have a structure most confidently similar to dynein motor proteins.
- Zapata-Bustos, R., Alonso-Castro, A. J., Gómez-Sánchez, M., & Salazar-Olivo, L. A. (2014). Ibervillea sonorae (Cucurbitaceae) induces the glucose uptake in human adipocytes by activating a PI3K-independent pathway. Journal of ethnopharmacology, 152(3), 546-52.More infoIbervillea sonorae (S. Watson) Greene (Cucurbitaceae), a plant used for the empirical treatment of type 2 diabetes in México, exerts antidiabetic effects on animal models but its mechanism of action remains unknown. The aim of this study is to investigate the antidiabetic mechanism of an Ibervillea sonorae aqueous extract (ISE).
- Alonso-Castro, A. J., Zapata-Bustos, R., Gómez-Espinoza, G., & Salazar-Olivo, L. A. (2012). Isoorientin reverts TNF-α-induced insulin resistance in adipocytes activating the insulin signaling pathway. Endocrinology, 153(11), 5222-30.More infoIsoorientin (ISO) is a plant C-glycosylflavonoid with purported antidiabetic effects but unexplored mechanisms of action. To gain insight into its antidiabetic mechanisms, we assayed nontoxic ISO concentrations on the 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2-deoxy-d-glucose (2-NBDG) uptake by murine 3T3-F442A and human sc adipocytes. In insulin-sensitive adipocytes, ISO stimulated the 2-NBDG uptake by 210% (murine) and 67% (human), compared with insulin treatment. Notably, ISO also induced 2-NBDG uptake in murine (139%) and human (60%) adipocytes made resistant to insulin by treatment with TNF-α, compared with the incorporation induced in these cells by rosiglitazone. ISO induction of glucose uptake in adipocytes was abolished by inhibitors of the insulin signaling pathway. These inhibitors also blocked the proper phosphorylation of insulin signaling pathway components induced by ISO in both insulin-sensitive and insulin-resistant adipocytes. Additionally, ISO stimulated the transcription of genes encoding components of insulin signaling pathway in murine insulin-sensitive and insulin-resistant adipocytes. In summary, we show here that ISO exerts its antidiabetic effects by activating the insulin signaling pathway in adipocytes, reverts the insulin resistance caused in these cells by TNF-α by stimulating the proper phosphorylation of proteins in this signaling pathway, and induces the expression of genes encoding these proteins.
- Ortiz-Andrade, R., Cabañas-Wuan, A., Arana-Argáez, V. E., Alonso-Castro, A. J., Zapata-Bustos, R., Salazar-Olivo, L. A., Domínguez, F., Chávez, M., Carranza-Álvarez, C., & García-Carrancá, A. (2012). Antidiabetic effects of Justicia spicigera Schltdl (Acanthaceae). Journal of ethnopharmacology, 143(2), 455-62.More infoJusticia spicigera is a plant species used for the Teenak (Huesteca Potosina) and Mayan (Yucatan peninsula) indigenous for the empirical treatment of diabetes, infections and as stimulant.
- Alonso-Castro, A. J., Zapata-Bustos, R., Domínguez, F., García-Carrancá, A., & Salazar-Olivo, L. A. (2011). Magnolia dealbata Zucc and its active principles honokiol and magnolol stimulate glucose uptake in murine and human adipocytes using the insulin-signaling pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 18(11), 926-33.More infoSome Magnolia (Magnoliaceae) species are used for the empirical treatment of diabetes mellitus, but the antidiabetic properties of Magnolia dealbata have not yet been experimentally validated. Here we report that an ethanolic extract of Magnolia dealbata seeds (MDE) and its active principles honokiol (HK) and magnolol (MG) induced the concentration-dependent 2-NBDG uptake in murine 3T3-F442A and human subcutaneous adipocytes. In insulin-sensitive adipocytes, MDE 50 μg/ml induced the 2-NBDG uptake by 30% respect to insulin, while HK and MG, 30 μM each, did it by 50% (murine) and 40% (human). The simultaneous application of HK and MG stimulated 2-NBDG uptake by 70% in hormone-sensitive cells, on which Magnolia preparations exerted synergic effects with insulin. In insulin-resistant adipocytes, MDE, HK and MG induced 2-NBDG uptake by 57%, 80% and 96% respect to Rosiglitazone (RGZ), whereas HK and MG simultaneously applied stimulated 2-NBDG uptake more efficiently than RGZ (120%) in both murine and human adipocytes. Inhibitors of the insulin-signaling pathway abolished the glucose uptake induced by Magnolia dealbata preparations, suggesting that their antidiabetic effects are mediated by this signaling pathway. In addition, MDE, HK and MG exerted only mild to moderate proadipogenic effects on 3T3-F442A and human preadipocytes, although the combined application of HK and MG markedly increased the lipid accumulation in both cell types. In summary, Magnolia dealbata and its active principles HK and MG stimulate glucose uptake in insulin-sensitive and insulin-resistant murine and human adipocytes using the insulin signaling pathway.
- Jacobo-Salcedo, M. d., Alonso-Castro, A. J., Salazar-Olivo, L. A., Carranza-Alvarez, C., González-Espíndola, L. A., Domínguez, F., Maciel-Torres, S. P., García-Lujan, C., González-Martínez, M. d., Gómez-Sánchez, M., Estrada-Castillón, E., Zapata-Bustos, R., Medellin-Milán, P., & García-Carrancá, A. (2011). Antimicrobial and cytotoxic effects of Mexican medicinal plants. Natural product communications, 6(12), 1925-8.More infoThe antimicrobial effects of the Mexican medicinal plants Guazuma ulmifolia, Justicia spicigera, Opuntia joconostle, O. leucotricha, Parkinsonia aculeata, Phoradendron longifolium, P. serotinum, Psittacanthus calyculatus, Tecoma stans and Teucrium cubense were tested against several human multi-drug resistant pathogens, including three Gram (+) and five Gram (-) bacterial species and three fungal species using the disk-diffusion assay. The cytotoxicity of plant extracts on human cancer cell lines and human normal non-cancerous cells was also evaluated using the MTT assay. Phoradendron longifolium, Teucrium cubense, Opuntia joconostle, Tecoma stans and Guazuma ulmifolia showed potent antimicrobial effects against at least one multidrug-resistant microorganism (inhibition zone > 15 mm). Only Justicia spicigera and Phoradendron serotinum extracts exerted active cytotoxic effects on human breast cancer cells (IC50 < or = 30 microg/mL). The results showed that Guazuma ulmifolia produced potent antimicrobial effects against Candida albicans and Acinetobacter lwoffii, whereas Justicia spicigera and Phoradendron serotinum exerted the highest toxic effects on MCF-7 and HeLa, respectively, which are human cancer cell lines. These three plant species may be important sources of antimicrobial and cytotoxic agents.
- Alonso-Castro, A. J., Zapata-Bustos, R., Romo-Yañez, J., Camarillo-Ledesma, P., Gómez-Sánchez, M., & Salazar-Olivo, L. A. (2010). The antidiabetic plants Tecoma stans (L.) Juss. ex Kunth (Bignoniaceae) and Teucrium cubense Jacq (Lamiaceae) induce the incorporation of glucose in insulin-sensitive and insulin-resistant murine and human adipocytes. Journal of ethnopharmacology, 127(1), 1-6.More infoTecoma stans (L.) Juss. ex Kunth (Bignoniaceae) and Teucrium cubense Jacq (Lamiaceae) are plants extensively used for the empirical treatment of diabetes mellitus, but their antidiabetic mechanisms remain to be clarified. In this study, the effect of aqueous extracts of Tecoma stans (TSE) and Teucrium cubense (TCE) on the glucose uptake in adipose cells was evaluated.
- Herrera-Herrera, M. L., Zapata-Bustos, R., & Salazar-Olivo, L. A. (2009). Simplified culture techniques for growth and differentiation of murine and human pre-adipocytes for translational applications. Cytotherapy, 11(1), 52-60.More infoAdipose tissue has become a promising source of adult stem cells. Looking for optimal culture conditions, we evaluated the ability of L15, a free-gas exchange culture medium, to support cell proliferation and adipogenesis of murine 3T3-F442A and human normal (HNPA) and lipoma-derived (HLPA) pre-adipocytes.
- Zapata Bustos, R. (2018, May). Evaluation of the exercise-induced transcriptional program regulation in lean and obese subjects. Endocrinology Journal Club Works in Progress. Tucson, Arizona: Department of Medicine, Division of Endocrinology, University of Arizona.More infoZapata-Bustos R. Evaluation of the exercise-induced transcriptional program regulation in lean and obese subjects. Department of Medicine, Division of Endocrinology, University of Arizona. Endocrinology Journal Club Works in Progress, May 08, 2018
- Zapata Bustos, R. (2017, February). Proteomic Analysis of the Vastus Lateralis from Lean and Obese Subjects: Evaluation of the Acetylome in Response to Exercise. Endocrinology Journal Club Works in Progress. Tucson, Arizona: Department of Medicine, Division of Endocrinology, University of Arizona..More infoZapata-Bustos R. Proteomic Analysis of the Vastus Lateralis from Lean and Obese Subjects: Evaluation of the Acetylome in Response to Exercise. Department of Medicine, Division of Endocrinology, University of Arizona. Endocrinology Journal Club Works in Progress, February 28, 2017
- Zapata Bustos, R. (2016, November). Proteomic Analysis of the Vastus Lateralis from Lean and Obese Subjects: Evaluation of the Acetylome in Response to Exercise.. Endocrinology Journal Club Works in Progress. Tucson, Arizona: Department of Medicine, Division of Endocrinology, University of Arizona.More infoZapata-Bustos R. Proteomic Analysis of the Vastus Lateralis from Lean and Obese Subjects: Evaluation of the Acetylome in Response to Exercise. Department of Medicine, Division of Endocrinology, University of Arizona. Endocrinology Journal Club Works in Progress, November 1, 2016
- Zapata Bustos, R. (2015, January). PGC-1 and muscle mitochondrial dysfunction in diabetes. Mayo/ASU Center for Metabolic and Vascular Biology Weekly Journal Club.. Scottsdale, Arizona: Mayo/ASU Center for Metabolic and Vascular Biology.More infoZapata-Bustos R. PGC-1 and muscle mitochondrial dysfunction in diabetes. Mayo/ASU Center for Metabolic and Vascular Biology Weekly Journal Club. January 28, 2015