Timothy A Bolger
- Assistant Professor, Molecular and Cellular Biology
- Assistant Professor, Genetics - GIDP
- Ph.D. Molecular Cancer Biology
- Duke University, Durham, North Carolina
- The function and regulation of histone deactylase-4 in neurons
- B.A. Biochemical Sciences
- Harvard University, Cambridge, Massachusetts
- Characterization of the transcriptional activation domain of Drosophila erect wing gene
- Assistant Professor, Department of Molecular and Cellular Biology, University of Arizona (2014 - Ongoing)
- Assistant Research Professor, Department of Molecular and Cellular Biology, University of Arizona (2011 - 2014)
- Postdoctoral Fellow, Department of Cell and Developmental Biology, Vanderbilt University (2006 - 2011)
- Research Assistant I, Department of Pathology, Harvard University (1999 - 2000)
- Undergraduate Summer Researcher, Department of Pathology, Harvard University (1998)
- Summer Research Aide, Virginia Institute of Marine Science, College of William and Mary (1997)
- Outstanding Faculty Mentor (Honorable Mention)
- Undergraduate Biology Research Program (UBRP), Fall 2013 (Award Finalist)
- Kirchstein National Research Service Award
- National Institutes of Health, Fall 2007
- Postdoctoral Recruiting Incentive for Excellence
- Vanderbilt University Medical Center, Fall 2006
- Graduate Research Fellowship
- National Science Foundation, Fall 2002
Regulation of mRNP dynamics in gene expression:Eukaryotic gene expression is a fundamental cellular activity that is critical for cellular identity, function, and physiology. During gene expression, a messenger RNA (mRNA) is generated by transcription and undergoes a number of different steps, including splicing and nuclear processing, nucleocytoplasmic export and localization, translation, and decay. These steps result in dynamic changes to the RNA sequence, structure, and the cohort of proteins bound to the mRNA. Furthermore, these changes need to occur with the proper timing and in the correct sequence to avoid aberrant expression. Therefore elaborate regulation of mRNP dynamics is required for proper gene expression.At virtually every step in gene expression, members of a highly conserved protein family called the DEAD-box proteins are required for facilitating mRNP transitions by acting either as RNA helicases or as ribonucleoprotein (RNP) remodeling enzymes. Furthermore, by regulating their activity, the potential exists to control mRNAs in different subsets and in response to different conditions. Thus we hypothesize that the DEAD-box proteins exert overarching control of mRNP dynamics in gene expression.Our research in the Bolger lab has dual goals: 1. addressing fundamental biological questions, and 2. utilizing this knowledge to advance human health. The Bolger laboratory uses the budding yeast Saccharomyces cerevisiae as a model system, and takes advantage of the combination of genetics, biochemistry, and cell biology allowed by yeast work. Our long-term goal in fundamental biology is to uncover the regulation of mRNP dynamics. Specifically, we are focusing on the in vivo roles, molecular targets, and regulation of DEAD-box proteins. Our research will not only greatly increase our understanding of how these factors function in the control of gene expression but also may open up new avenues for therapies, either for cancer or for the other pathologies related to this research.
DissertationMCB 920 (Fall 2016)
Honors ThesisBIOC 498H (Fall 2016)
Integrative Approaches to BioMCB 585 (Fall 2016)
Lab Presentations & DiscussionMCB 696A (Fall 2016)
DissertationMCB 920 (Spring 2016)
Honors ThesisMCB 498H (Spring 2016)
Lab Presentations & DiscussionMCB 696A (Spring 2016)
Molecular GeneticsMCB 304 (Spring 2016)
DissertationMCB 920 (Fall 2015)
Honors ThesisMCB 498H (Fall 2015)
Integrative Approaches to BioMCB 585 (Fall 2015)
Lab Presentations & DiscussionMCB 696A (Fall 2015)
DissertationMCB 920 (Spring 2015)
Honors Independent StudyMCB 399H (Spring 2015)
Honors Independent StudyMCB 499H (Spring 2015)
Honors ThesisMCB 498H (Spring 2015)
Lab Presentations & DiscussionMCB 696A (Spring 2015)
Molecular GeneticsMCB 304 (Spring 2015)
ResearchMCB 900 (Spring 2015)
DissertationMCB 920 (Fall 2014)
Honors ThesisMCB 498H (Fall 2014)
Introduction to ResearchMCB 795A (Fall 2014)
Lab Presentations & DiscussionMCB 696A (Fall 2014)
ResearchMCB 900 (Fall 2014)
Honors ThesisBIOC 498H (Spring 2014)
Lab Presentations & DiscussionMCB 696A (Spring 2014)
ResearchMCB 900 (Spring 2014)
CBIO GIDP Seminar SeriesCBIO 596H (Fall 2013)
Honors ThesisBIOC 498H (Fall 2013)
Lab Presentations & DiscussionMCB 696A (Fall 2013)
ResearchMCB 900 (Fall 2013)
- Bolger, T. A., Cohen, T., & Yao, T. (2006). HATs and HDACs. In Gene Expression and Regulation (ed. J Ma)(pp p111-133). Beijing, China: Higher Education Press and Springer.
- Bolger, T. A., & Wente, S. R. (2011). Gle1 is a multifunctional DEAD-box protein regulator that modulates Ded1 in translation initiation. Journal of Biological Chemistry, 286(46), 39750-39759.More infoPMID: 21949122;PMCID: PMC3220593;Abstract: DEAD-box protein (Dbp) family members are essential for gene expression; however, their precise roles and regulation are not fully defined. During messenger (m)RNA export, Gle1 bound to inositol hexakisphosphate (IP 6) acts via Dbp5 to facilitate remodeling of mRNA-protein complexes. In contrast, here we define a novel Gle1 role in translation initiation through regulation of a different DEAD-box protein, the initiation factor Ded1. We find that Gle1 physically and genetically interacts with Ded1. Surprisingly, whereas Gle1 stimulates Dbp5, it inhibits Ded1 ATPase activity in vitro, and IP 6 does not affect this inhibition. Functionally, a gle1-4 mutant specifically suppresses initiation defects in a ded1-120 mutant, and ded1 and gle1 mutants have complementary perturbations in AUG start site recognition. Consistent with this role in initiation, Gle1 inhibits translation in vitro in competent extracts. These results indicate that Gle1 has a direct role in initiation and negatively regulates Ded1. Together, the differential regulation of two distinct DEAD-box proteins by a common factor (Gle1) establishes a new paradigm for controlling gene expression and coupling translation with mRNA export. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
- Alcázar-Román, A. R., Bolger, T. A., & Wente, S. R. (2010). Control of mRNA export and translation termination by inositol hexakisphosphate requires specific interaction with Gle. Journal of Biological Chemistry, 285(22), 16683-16692.More infoPMID: 20371601;PMCID: PMC2878036;Abstract: The unidirectional translocation of messenger RNA (mRNA) through the aqueous channel of the nuclear pore complex (NPC) is mediated by interactions between soluble mRNA export factors and distinct binding sites on the NPC. At the cytoplasmic side of the NPC, the conserved mRNA export factors Gle1 and inositol hexakisphosphate (IP6) play an essential role in mRNA export by activating the ATPase activity of the DEAD-box protein Dbp5, promoting localized messenger ribonucleoprotein complex remodeling, and ensuring the directionality of the export process. In addition, Dbp5, Gle1, and IP 6 are also required for proper translation termination. However, the specificity of the IP6-Gle1 interaction in vivo is unknown. Here, we characterize the biochemical interaction between Gle1 and IP6 and the relationship to Dbp5 binding and stimulation. We identify Gle1 residues required for IP6 binding and show that these residues are needed for IP6-dependent Dbp5 stimulation in vitro. Furthermore, we demonstrate that Gle1 is the primary target of IP6 for both mRNA export and translation termination in vivo. In Saccharomyces cerevisiae cells, the IP 6-binding mutants recapitulate all of the mRNA export and translation termination defects found in mutants depleted of IP6. We conclude that Gle1 specifically binds IP6 and that this interaction is required for the full potentiation of Dbp5 ATPase activity during both mRNA export and translation termination. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
- Bolger, T. A., Folkmann, A. W., Tran, E. J., & Wente, S. R. (2008). The mRNA Export Factor Gle1 and Inositol Hexakisphosphate Regulate Distinct Stages of Translation. Cell, 134(4), 624-633.More infoPMID: 18724935;PMCID: PMC2601711;Abstract: Gene expression requires proper messenger RNA (mRNA) export and translation. However, the functional links between these consecutive steps have not been fully defined. Gle1 is an essential, conserved mRNA export factor whose export function is dependent on the small molecule inositol hexakisphosphate (IP6). Here, we show that both Gle1 and IP6 are required for efficient translation termination in Saccharomyces cerevisiae and that Gle1 interacts with termination factors. In addition, Gle1 has a conserved physical association with the initiation factor eIF3, and gle1 mutants display genetic interactions with the eIF3 mutant nip1-1. Strikingly, gle1 mutants have defects in initiation, whereas strains lacking IP6 do not. We propose that Gle1 functions together with IP6 and the DEAD-box protein Dbp5 to regulate termination. However, Gle1 also independently mediates initiation. Thus, Gle1 is uniquely positioned to coordinate the mRNA export and translation mechanisms. These results directly impact models for perturbation of Gle1 function in pathophysiology. © 2008 Elsevier Inc. All rights reserved.
- Bolger, T. A., Zhao, X., Cohen, T. J., Tsai, C., & Yao, T. (2007). The neurodegenerative disease protein ataxin-1 antagonizes the neuronal survival function of myocyte enhancer factor-2. Journal of Biological Chemistry, 282(40), 29186-29192.More infoPMID: 17646162;Abstract: Ataxin-1 is a neurodegenerative disorder protein whose mutant form causes spinocerebellar ataxia type-1 (SCA1). Evidence suggests that ataxin-1 may function as a transcription repressor. However, neither the importance of this putative transcriptional repression activity in neural cytotoxicity nor the transcriptional targets of ataxin-1 are known. Here we identify the MEF2-HDAC4 transcriptional complex involved in neuron survival as a target of ataxin-1. We show that ataxin-1 binds specifically to histone deacetylase-4 (HDAC4) and MEF2 and colocalizes with them in nuclear inclusion bodies. Significantly, these interactions are greatly reduced by the S776A mutation, which largely abrogates the cytotoxicity of ataxin-1. Supporting the importance of these interactions, we show that wild type ataxin-1 represses MEF2-dependent transcription, whereas the S776A mutant is less potent. Furthermore, overexpression of MEF2 can partially reverse cytotoxicity caused by ataxin-1. Our results identify the MEF2-HDAC4 complex as a target for ataxin-1 transcriptional repression activity and suggest a novel pathogenic mechanism whereby ataxin-1 sequesters and inhibits the neuronal survival factor MEF2. © 2007 by The American Society for Biochemistry and Molecular Biology, Inc.
- Tran, E. J., Bolger, T. A., & Wente, S. R. (2007). SnapShot: Nuclear Transport. Cell, 131(2), 420.e1-420.e2.More infoPMID: 17956740;
- Bolger, T. A., & Yao, T. (2005). Intracellular trafficking of histone deacetylase 4 regulates neuronal cell death. Journal of Neuroscience, 25(41), 9544-9553.More infoPMID: 16221865;Abstract: Histone deacetylase 4 (HDAC4) undergoes signal-dependent shuttling between the cytoplasm and nucleus, which is regulated in part by calcium/calmodulin- dependent kinase (CaMK)-mediated phosphorylation. Here, we report that HDAC4 intracellular trafficking is important in regulating neuronal cell death. HDAC4 is normally localized to the cytoplasm in brain tissue and cultured cerebellar granule neurons (CGNs). However, in response to low-potassium or excitotoxic glutamate conditions that induce neuronal cell death, HDAC4 rapidly translocates into the nucleus of cultured CGNs. Treatment with the neuronal survival factor BDNF suppresses HDAC4 nuclear translocation, whereas a proapoptotic CaMK inhibitor stimulates HDAC4 nuclear accumulation. Moreover, ectopic expression of nuclear-localized HDAC4 promotes neuronal apoptosis and represses the transcriptional activities of myocyte enhancer factor 2 and cAMP response element-binding protein, survival factors in neurons. In contrast, inactivation of HDAC4 by small interfering RNA or HDAC inhibitors suppresses neuronal cell death. Finally, an increase of nuclear HDAC4 in granule neurons is also observed in weaver mice, which harbor a mutation that promotes CGN apoptosis. Our data identify HDAC4 and its intracellular trafficking as key effectors of multiple pathways that regulate neuronal cell death. Copyright © 2005 Society for Neuroscience.
- Zhao, X., Sternsdorf, T., Bolger, T. A., Evans, R. M., & Yao, T. (2005). Regulation of MEF2 by histone beacetylase 4- and SIRT1 deacetylase-mediated lysine modifications. Molecular and Cellular Biology, 25(19), 8456-8464.More infoPMID: 16166628;PMCID: PMC1265742;Abstract: The class II deacetylase histone deacetylase 4 (HDAC4) negatively regulates the transcription factor MEF2. HDAC4 is believed to repress MEF2 transcriptional activity by binding to MEF2 and catalyzing local histone deacetylation. Here we report that HDAC4 also controls MEF2 by a novel SUMO E3 ligase activity. We show that HDAC4 interacts with the SUMO E2 conjugating enzyme Ubc9 and is itself sumoylated. The overexpression of HDAC4 leads to prominent MEF2 sumoylation in vivo, whereas recombinant HDAC4 stimulates MEF2 sumoylation in a reconstituted system in vitro. Importantly, HDAC4 promotes sumoylation on a lysine residue that is also subject to acetylation by a MEF2 coactivator, the acetyltransferase CBP, suggesting a possible interplay between acetylation and sumoylation in regulating MEF2 activity. Indeed, MEF2 acetylation is correlated with MEF2 activation and dynamically induced upon muscle cell differentiation, while sumoylation inhibits MEF2 transcriptional activity. Unexpectedly, we found that HDAC4 does not function as a MEF2 deacetylase. Instead, the NAD+-dependent deacetylase SIRT1 can potently induce MEF2 deacetylation. Our studies reveal a novel regulation of MEF2 transcriptional activity by two distinct classes of deacetylases that affect MEF2 sumoylation and acetylation. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
- Fazio, I. K., Bolger, T. A., & Gill, G. (2001). Conserved Regions of the Drosophila Erect Wing Protein Contribute Both Positively and Negatively to Transcriptional Activity. Journal of Biological Chemistry, 276(22), 18710-18716.More infoPMID: 11278998;Abstract: Genetic studies of the Drosophila erect wing (ewg) gene have revealed that ewg has an essential function in the embryonic nervous system and is required for the specification of certain muscle cells. We have found that EWG is a site-specific transcriptional activator, and we report here that evolutionarily conserved regions of EWG contribute both positively and negatively to transcriptional activity. Using gel mobility shift assays, we have shown that an EWG dimer binds specifically to DNA. In transfection assays, EWG activated expression of a reporter gene bearing specific binding sites. Analysis of deletion mutants and fusions of EWG to the Gal4 DNA binding domain has identified a transcriptional activation domain in the C terminus of EWG. Deletion analysis also revealed a novel inhibitory region in the N terminus of EWG. Strikingly, both the activation domain and the inhibitory region are conserved in EWG homologs including human nuclear respiratory factor 1 (NRF-1) and the sea urchin P3A2 protein. The strong conservation of elements that determine transcriptional activity suggests that the EWG, NRF-1, and P3A2 family of proteins shares common mechanisms of action and has maintained common functions across evolution.
- Zhao, X., Ito, A., Kane, C. D., Liao, T., Bolger, T. A., Lemrow, S. M., Means, A. R., & Yao, T. (2001). The Modular Nature of Histone Deacetylase HDAC4 Confers Phosphorylation-dependent Intracellular Trafficking. Journal of Biological Chemistry, 276(37), 35042-35048.More infoPMID: 11470791;Abstract: In C2C12 myoblasts, endogenous histone deacetylase HDAC4 shuttles between cytoplasmic and nuclear compartments, supporting the hypothesis that its subcellular localization is dynamically regulated. However, upon differentiation, this dynamic equilibrium is disturbed and we find that HDAC4 accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. Consistent with the notion of regulation of HDAC4 intracellular trafficking, we reveal that HDAC4 contains a modular structure consisting of a C-terminal autonomous nuclear export domain, which, in conjunction with an internal regulatory domain responsive to calcium/calmodulin-dependent protein kinase IV (CaMKIV), determines its subcellular localization. CaMKIV phosphorylates HDAC4 in vitro and promotes its nuclear-cytoplasmic shuttling in vivo. However, although 14-3-3 binding of HDAC4 has been proposed to be important for its cytoplasmic retention, we find this interaction to be independent of CaMKIV. Rather, the HDAC4·14-3-3 complex exists in the nucleus and is required to confer CaMKIV responsiveness. Our results suggest that the subcellular localization of HDAC4 is regulated by sequential phosphorylation events. The first event is catalyzed by a yet to be identified protein kinase that promotes 14-3-3 binding, and the second event, involving protein kinases such as CaMKIV, leads to efficient nuclear export of the HDAC4·14-3-3 complex.
- Bolger, T. A. (2015, March). D-E-A-D and Alive: Controlling mRNA-Protein Dynamics in Normal and Cancer Cells. Basic Medical Sciences Seminar Series, College of Medicine-Phoenix. Phoenix, AZ.
- Bolger, T. A. (2014, Nov). D-E-A-D and Alive: Controlling mRNA-Protein Dynamics in Normal and Cancer Cells. RISE Research Colloquium, SF State Univ. San Francisco, CA: San Francisco State University.
- Bolger, T. A. (2013, Sept). Control of mRNP dynamics by Ded1, a cancer-related RNA helicase.. Cancer Biology Seminar Series. Tucson, AZ: University of Arizona Cancer Center.
- Bolger, T. A. (2012, Oct). The control of gene expression through regulation of DEAD-box RNA helicases.. Microlunch Seminar Series. Tucson, AZ: Department of Veterinary Science and Microbiology, University of Arizona.
- Bolger, T. A. (2012, Sept). Control of gene expression by regulation of DEAD-box RNA helicases.. Joint Biology Research Retreat. Oracle, AZ: MCB/CMM/CBC/IMB.
- Bolger, T. A. (2010, Apr). Gle1 is a versatile regulator of DEAD-Box proteins in mRNA export and translation.. Departmental Annual Retreat. Nashville, TN: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine.
- Bolger, T. A. (2010, June). Gle1 is a versatile regulator of DEAD-Box proteins in mRNA export and translation.. Annual Meeting of the RNA Society. Seattle, WA: RNA Society.
- Bolger, T. A. (2008, Aug). The mRNA export factor Gle1 and inositol hexakisphosphate regulate distinct stages of translation.. Meeting on Translational Control. Cold Spring Harbor, NY: Cold Spring Harbor Laboratories.
- Bolger, T. A. (2004, Sept). Histone deacetylase 4 promotes neuronal cell death.. Department Annual Symposium. Wilmington, NC: Department of Pharmacology and Cancer Biology, Duke University School of Medicine.
- Bolger, T. A. (2016, Sept). Control of mRNA fate through dynamic regulation of DEAD-box helicases: Regulation of Ded1 function in translation by Gle1. Cold Spring Harbor Meeting on Translational Control. Cold Spring Harbor, NY: CSHL.
- Aryanpur, P. P., Regan, C. A., Vergara, A. M., & Bolger, T. A. (2014, Jun). Control of mRNA fate through dynamic regulation of DEAD-box helicases: Regulation of Ded1 function in translation by Gle1. RNA Society Annual Meeting. Quebec City, Quebec, Canada: RNA Society.
- Regan, C. A., Langston, R., & Bolger, T. A. (2012, Dec). Control of gene expression through regulation of DEAD-box helicases: the function of Gle1 in mRNA export and translation. Annual Meeting of the American Society for Cell Biology. San Francisco, CA: American Society for Cell Biology.
- Bolger, T. A. (2009, Aug). Functional coupling of mRNA export and translation for control of gene expression. International Meeting on Nuclear Trafficking. Banff, Alberta, Canada.