Andrew R Rouse

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Courses

Scholarly Contributions

Books

• Kupinski, M., Rouse, A., & Gmitro, A. (2016). Applying J-optimal Channelized Quadratic Observers (J-CQO) to a Clinical Imaging Study for Ovarian Cancer Detection.

Journals/Publications

• Gmitro, A. F., Larson, M. C., Klein, R., Rouse, A. R., Woodhead, G. J., & Li, Z. (2023). Multispectral confocal endomicroscopy in lung biopsy guidance. Journal of Optical Microsystems, 3(1). doi:10.1117/1.JOM.3.1.011002
• Barton, J. K., Hennemeyer, C. T., Carlson, Q., Woodhead, G. J., Rouse, A. R., Utzinger, U., Gmitro, A. F., & Larson, M. (2021). Using FDA-approved drugs as off-label fluorescent dyes for optical biopsies: from in silico design to ex vivo proof-of-concept. Methods and Applications in Fluorescence.
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  AbstractOptical biopsies bring the microscope to the patient rather than the tissue to the microscope, and may complement or replace the tissue-harvesting component of the traditional biopsy process with its associated risks. In general, optical biopsies are limited by the lack of endogenous tissue contrast and the small number of clinically approved in vivo dyes. This study tests multiple FDA-approved drugs that have structural similarity to research dyes as off-label in situ fluorescent alternatives to standard ex vivo hematoxylin & eosin tissue stain. Numerous drug-dye combinations shown here may facilitate relatively safe and fast in situ or possibly in vivo staining of tissue, enabling real-time optical biopsies and other advanced microscopy technologies, which have implications for the speed and performance of tissue- and cellular-level diagnostics.
• Rouse, A. R., Barton, J. K., Utzinger, U., Woodhead, G. J., Hennemeyer, C. T., Gmitro, A. F., Larson, M., & Carlson, Q. (2021). Using FDA-approved drugs as off-label fluorescent dyes for optical biopsies: from in silico design to ex vivo proof-of-concept. Methods and Applications in Fluorescence.
• Momsen, N. C., Rouse, A. R., & Gmitro, A. F. (2020). Improvement in optical fiber bundle-based imaging using synchronized fiber motion. Applied optics, 59(22), G249-G254.
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  Image quality in fiber bundle-based imaging systems is inherently limited by the size and spacing of the individual fiber cores. The fiber bundle limits the achievable spatial resolution and superimposes a fixed pattern of signal variability across the image. To overcome these limitations, piezoelectric tubes were used to synchronously dither the fiber bundle on both ends. Experimental results using the dithering approach with a commercial fiber bundle showed a substantial decrease in fixed pattern noise and an increase in spatial resolution.
• Risi, M. D., Rouse, A. R., Chambers, S. K., Hatch, K. D., Zheng, W., & Gmitro, A. F. (2016). Pilot Clinical Evaluation of a Confocal Microlaparoscope for Ovarian Cancer Detection. International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, 26(2), 248-54.
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  The aim of this study is to evaluate the performance of a confocal fluorescence microlaparoscope for in vivo detection of ovarian cancer.
• Woolfenden, J. M., Pak, K. Y., Witte, M. H., Gmitro, A. F., Liang, R., Banerjee, B., Patel, C., Rouse, A. R., Furenlid, L. R., Bernas, M., Cai, M., Barber, C., Gray, B. D., & Liu, Z. (2016). Characterization of TCP-1 molecular imaging probes in mouse models with xenografted human colon cancer.. J Control Release, 239, 223-230.
• Phung, M., Rouse, A., Pangilinan, J., Bell, R., Bracamonte, E., Mashi, S., Gmitro, A., & Lee, B. (2020). Investigation of confocal microscopy for differentiation of renal cell carcinoma versus benign tissue. Can an optical biopsy be performed?. Asian Journal of Urology, 7(4).
• Risi, M. D., Makhlouf, H., Rouse, A. R., & Gmitro, A. F. (2015). Analysis of multimode fiber bundles for endoscopic spectral-domain optical coherence tomography. Applied optics, 54(1), 101-13.
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  A theoretical analysis of the use of a fiber bundle in spectral-domain optical coherence tomography (OCT) systems is presented. The fiber bundle enables a flexible endoscopic design and provides fast, parallelized acquisition of the OCT data. However, the multimode characteristic of the fibers in the fiber bundle affects the depth sensitivity of the imaging system. A description of light interference in a multimode fiber is presented along with numerical simulations and experimental studies to illustrate the theoretical analysis.

Proceedings Publications

• Bearman, G. H., Descour, M. R., Ford, B. K., Gmitro, A. F., Lynch, R. M., Rouse, A. R., Sabharwal, Y. S., & Volin, C. E. (1999). Video-rate spectral imaging system for fluorescence microscopy. In SPIE Proceedings, 3603, 216-226.
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  We describe fluorescence spectral-imaging results with the computed-tomography imaging spectrometer (CTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, the CTIS can be used to image dynamic phenomena involving multiple, spectrally overlapping fluorescence probes. This system is also optimal for simultaneously monitoring changes in spectral characteristics of multiple probes from different locations within the same sample. This advantage will provide additional information about the physiological changes in function form populations of cells which respond in a heterogeneous manner. The results presented in this paper consist of proof-of-concept imaging results from the CTIS in combination with two different systems of fore- optics. In the first configuration, raw image data were collected using the CTIS coupled to an inverted fluorescence microscope. The second configuration combined the CTIS with a confocal microscope equipped with a fiber-optic imaging bundle, previously for in vivo imaging. Image data were collected at frame rates of 15 frame per second and emission spectra were sample at 10-nm intervals with a minimum of 29 spectral bands. The smallest spatial sampling interval presented in this paper is 0.7 micrometers .

Presentations

• Lee, B. R., Gmitro, A. F., Price, E., Bracamonte, E. R., Bell, R., Rouse, A. R., & Phung, M. (2018, September). Optical Biopsy using Confocal Microscopy for Differentiation of Renal Cell Carcinoma versus Benign Tissue. American Urological Association National Meeting. Paris, France: World Congress of Endourology.
• Phung, M., Phung, M., Rouse, A. R., Rouse, A. R., Bell, R., Bell, R., Bracamonte, E. R., Bracamonte, E. R., Price, E., Price, E., Gmitro, A. F., Gmitro, A. F., Lee, B. R., & Lee, B. R. (2018, May). Optical Biopsy using Confocal Microscopy for Differentiation of Renal Cell Carcinoma versus Benign Tissue. American Urological Association National Meeting. San Francisco: American Urological Association.
• Gmitro, A. F., Zheng, W., Hatch, K. D., Chambers, S. K., Risi, M. D., & Rouse, A. R. (2015, February). Clinical experience with a confocal microlaparoscope for ovarian cancer detection. SPIE BIOS: Endoscopic Microscopy X. San Francisco, CA.