Denise Roe

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• Adams, A. C., Macy, A. M., Borden, E. S., Herrmann, L. M., Brambley, C. A., Ma, T., Li, X., Hughes, A., Roe, D. J., Mangold, A. R., Buetow, K. H., Wilson, M. A., Baker, B. M., & Hastings, K. T. (2024). Distinct sets of molecular characteristics define tumor-rejecting neoantigens. bioRxiv : the preprint server for biology.
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  Challenges in identifying tumor-rejecting neoantigens limit the efficacy of neoantigen vaccines to treat cancers, including cutaneous squamous cell carcinoma (cSCC). A minority of human cSCC tumors shared neoantigens, supporting the need for personalized vaccines. Using a UV-induced mouse cSCC model which recapitulated the mutational signature and driver mutations found in human disease, we found that CD8 T cells constrain cSCC. Two MHC class I neoantigens were identified that constrained cSCC growth. Compared to the wild-type peptides, one tumor-rejecting neoantigen exhibited improved MHC binding and the other had increased solvent accessibility of the mutated residue. Across known neoantigens that do not impact MHC binding, structural modeling of the peptide/MHC complexes indicated that increased solvent accessibility, which will facilitate TCR recognition of the neoantigen, distinguished tumor-rejecting from non-immunogenic neoantigens. This work reveals characteristics of tumor-rejecting neoantigens that may be of considerable importance in identifying optimal vaccine candidates in cSCC and other cancers.
• Bixby, B., Vrba, L., Lenka, J., Oshiro, M. M., Watts, G. S., Hughes, T., Erickson, H., Chopra, M., Knepler, J. L., Knox, K. S., Jarnagin, L., Alalawi, R., Kala, M., Bernert, R., Routh, J., Roe, D. J., Garland, L. L., Futscher, B. W., & Nelson, M. A. (2024). Cell-free DNA methylation analysis as a marker of malignancy in pleural fluid. Scientific reports, 14(1), 2939.
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  Diagnosis of malignant pleural effusion (MPE) is made by cytological examination of pleural fluid or histological examination of pleural tissue from biopsy. Unfortunately, detection of malignancy using cytology has an overall sensitivity of 50%, and is dependent upon tumor load, volume of fluid assessed, and cytopathologist experience. The diagnostic yield of pleural fluid cytology is also compromised by low abundance of tumor cells or when morphology is obscured by inflammation or reactive mesothelial cells. A reliable molecular marker that may complement fluid cytology for the diagnosis of malignant pleural effusion is needed. The purpose of this study was to establish a molecular diagnostic approach based on pleural effusion cell-free DNA methylation analysis for the differential diagnosis of malignant pleural effusion and benign pleural effusion. This was a blind, prospective case-control biomarker study. We recruited 104 patients with pleural effusion for the study. We collected pleural fluid from patients with: MPE (n = 48), indeterminate pleural effusion in subjects with known malignancy or IPE (n = 28), and benign PE (n = 28), and performed the Sentinel-MPE liquid biopsy assay. The methylation level of Sentinel-MPE was markedly higher in the MPE samples compared to BPE control samples (p 
• Dickinson, S. E., Vaishampayan, P., Jandova, J., Ai, Y. E., Kirschnerova, V., Zhang, T., Calvert, V., Petricoin, E., Chow, H. S., Hu, C., Roe, D., Bode, A., Curiel-Lewandrowski, C., & Wondrak, G. T. (2024). Inhibition of UV-Induced Stress Signaling and Inflammatory Responses in SKH-1 Mouse Skin by Topical Small-Molecule PD-L1 Blockade. JID innovations : skin science from molecules to population health, 4(2), 100255.
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  The immune checkpoint ligand PD-L1 has emerged as a molecular target for skin cancer therapy and might also hold promise for preventive intervention targeting solar UV light-induced skin damage. In this study, we have explored the role of PD-L1 in acute keratinocytic photodamage testing the effects of small-molecule pharmacological inhibition. Epidermal PD-L1 upregulation in response to chronic photodamage was established using immunohistochemical and proteomic analyses of a human skin cohort, consistent with earlier observations that PD-L1 is upregulated in cutaneous squamous cell carcinoma. Topical application of the small-molecule PD-L1 inhibitor BMS-202 significantly attenuated UV-induced activator protein-1 transcriptional activity in SKH-1 bioluminescent reporter mouse skin, also confirmed in human HaCaT reporter keratinocytes. RT-qPCR analysis revealed that BMS-202 antagonized UV induction of inflammatory gene expression. Likewise, UV-induced cleavage of procaspase-3, a hallmark of acute skin photodamage, was attenuated by topical BMS-202. NanoString nCounter transcriptomic analysis confirmed downregulation of cutaneous innate immunity- and inflammation-related responses, together with upregulation of immune response pathway gene expression. Further mechanistic analysis confirmed that BMS-202 antagonizes UV-induced PD-L1 expression both at the mRNA and protein levels in SKH-1 epidermis. These data suggest that topical pharmacological PD-L1 antagonism using BMS-202 shows promise for skin protection against photodamage.
• Bauman, J. E., Saba, N. F., Roe, D., Bauman, J. R., Kaczmar, J., Bhatia, A., Muzaffar, J., Julian, R., Wang, S., Bearelly, S., Baker, A., Steuer, C., Giri, A., Burtness, B., Centuori, S., Caulin, C., Klein, R., Saboda, K., Obara, S., & Chung, C. H. (2023). Randomized Phase II Trial of Ficlatuzumab With or Without Cetuximab in Pan-Refractory, Recurrent/Metastatic Head and Neck Cancer. Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 41(22), 3851-3862.
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  Primary or acquired resistance to cetuximab, an antiepidermal growth factor receptor monoclonal antibody (mAb), minimizes its utility in recurrent/metastatic head and neck squamous cell carcinoma (HNSCC). Aberrant hepatocyte growth factor/cMet pathway activation is an established resistance mechanism. Dual pathway targeting may overcome resistance.
• Bea, J. W., de Heer, H. '., Lane, T., Charley, B., Yazzie, E., Yellowhair, J., Hudson, J., Kinslow, B., Wertheim, B. C., Roe, D. J., & Schwartz, A. (2023). Restoring Balance: a physical activity intervention for Native American cancer survivors and their familial support persons. Exercise, sport, & movement, 1(2).
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  Exercise interventions among Native American cancer survivors are lacking, despite major cancer health disparities in survivorship. The purpose of this study was to evaluate a 12-week randomized controlled trial (RCT) of culturally tailored exercise on cancer risk biomarkers and quality of life among Native American cancer survivors and family members.
• Bixby, B., Vrba, L., Lenka, J., Oshiro, M., Watts, G. S., Hughes, T., Erickson, H., Chopra, M., Knepler, J. L., Knox, K. S., Jarnagin, L., Alalawi, R., Kala, M., Bernert, R., Routh, J., Roe, D. J., Garland, L. L., Futscher, B. W., & Nelson, M. A. (2023). Cell-Free DNA Methylation Analysis as a Marker of Malignancy in Pleural Fluid. Research square.
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  Diagnosis of malignant pleural effusion (MPE) is made by cytological examination of pleural fluid or histological examination of pleural tissue from biopsy. Unfortunately, detection of malignancy using cytology has an overall sensitivity of 50%, and is dependent upon tumor load, volume of fluid assessed, and cytopathologist experience. The diagnostic yield of pleural fluid cytology is also compromised by low abundance of tumor cells or when morphology is obscured by inflammation or reactive mesothelial cells. A reliable molecular marker that may complement fluid cytology malignant pleural effusion diagnosis is needed. The purpose of this study was to establish a molecular diagnostic approach based on pleural effusion cell-free DNA methylation analysis for the differential diagnosis of malignant pleural effusion and benign pleural effusion.
• Chase, D. M., McCann, L. D., Treuth, A., Cui, H., Laniewski, P., Jimenez, N. R., Mahnert, N. D., Roe, D. J., & Herbst-Kralovetz, M. M. (2023). Preoperative quality of life at time of gynecologic surgery: considerations for postoperative management. AJOG global reports, 3(4), 100275.
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  Patients presenting for gynecologic surgery are a heterogeneous group. Preoperative quality of life may be a useful tool to guide postoperative management.
• Martinez, J. A., Wertheim, B. C., Roe, D. J., Taljanovic, M. S., Chow, H. S., Chew, W., Ehsani, S., Jiralerspong, S., Segar, J., & Chalasani, P. (2023). Oxylipins as Biomarkers for Aromatase Inhibitor-Induced Arthralgia (AIA) in Breast Cancer Patients. Metabolites, 13(3).
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  Aromatase inhibitor-induced arthralgia (AIA) presents a major problem for patients with breast cancer but is poorly understood. This prospective study explored the inflammatory metabolomic changes in the development of AIA. This single-arm, prospective clinical trial enrolled 28 postmenopausal women with early-stage (0-3) ER+ breast cancer starting adjuvant anastrozole. Patients completed the Breast Cancer Prevention Trial (BCPT) Symptom Checklist and the Western Ontario and McMaster Universities Arthritis Index (WOMAC) at 0, 3, and 6 months. The plasma levels of four polyunsaturated fatty acids (PUFAs) and 48 oxylipins were quantified at each timepoint. The subscores for WOMAC-pain and stiffness as well as BCPT-total, hot flash, and musculoskeletal pain significantly increased from baseline to 6 months (all < 0.05). PUFA and oxylipin levels were stable over time. The baseline levels of 8-HETE were positively associated with worsening BCPT-total, BCPT-hot flash, BCPT-musculoskeletal pain, WOMAC-pain, and WOMAC- stiffness at 6 months (all < 0.05). Both 9-HOTrE and 13(S)-HOTrE were related to worsening hot flash, and 5-HETE was related to worsening stiffness (all < 0.05). This is the first study to prospectively characterize oxylipin and PUFA levels in patients with breast cancer starting adjuvant anastrozole. The oxylipin 8-HETE should be investigated further as a potential biomarker for AIA.
• Parker, S. S., Ly, K. T., Grant, A. D., Sweetland, J., Wang, A. M., Parker, J. D., Roman, M. R., Saboda, K., Roe, D. J., Padi, M., Wolgemuth, C. W., Langlais, P., & Mouneimne, G. (2023). EVL and MIM/MTSS1 regulate actin cytoskeletal remodeling to promote dendritic filopodia in neurons. The Journal of cell biology, 222(5).
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  Dendritic spines are the postsynaptic compartment of a neuronal synapse and are critical for synaptic connectivity and plasticity. A developmental precursor to dendritic spines, dendritic filopodia (DF), facilitate synapse formation by sampling the environment for suitable axon partners during neurodevelopment and learning. Despite the significance of the actin cytoskeleton in driving these dynamic protrusions, the actin elongation factors involved are not well characterized. We identified the Ena/VASP protein EVL as uniquely required for the morphogenesis and dynamics of DF. Using a combination of genetic and optogenetic manipulations, we demonstrated that EVL promotes protrusive motility through membrane-direct actin polymerization at DF tips. EVL forms a complex at nascent protrusions and DF tips with MIM/MTSS1, an I-BAR protein important for the initiation of DF. We proposed a model in which EVL cooperates with MIM to coalesce and elongate branched actin filaments, establishing the dynamic lamellipodia-like architecture of DF.
• Yang, J., Lim, J. T., Victor, P., Chen, C., Khwaja, H., Schnellmann, R. G., Roe, D. J., Gokhale, P. C., DeCaprio, J. A., & Padi, M. (2023). Integrative analysis reveals therapeutic potential of pyrvinium pamoate in Merkel cell carcinoma. bioRxiv : the preprint server for biology.
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  Merkel Cell Carcinoma (MCC) is a highly aggressive neuroendocrine cutaneous malignancy arising from either ultraviolet-induced mutagenesis or Merkel cell polyomavirus (MCPyV) integration. It is the only known neuroendocrine tumor (NET) with a virus etiology. Despite extensive research, our understanding of the molecular mechanisms driving the transition from normal cells to MCC remains limited. To address this knowledge gap, we assessed the impact of inducible MCPyV T antigens into normal human fibroblasts by performing RNA sequencing. Our findings suggested that the WNT signaling pathway plays a critical role in the development of MCC. To test this model, we bioinformatically evaluated various perturbagens for their ability to reverse the MCC gene expression signature and identified pyrvinium pamoate, an FDA-approved anthelminthic drug known for its anti-tumor potential in multiple cancers. Leveraging transcriptomic, network, and molecular analyses, we found that pyrvinium effectively targets multiple MCC vulnerabilities. Specifically, pyrvinium not only reverses the neuroendocrine features of MCC by modulating canonical and non-canonical WNT signaling pathways but also inhibits cancer cell growth by activating the p53-mediated apoptosis pathway, disrupting mitochondrial function, and inducing endoplasmic reticulum (ER) stress. Pyrvinium also effectively inhibits tumor growth in an MCC mouse xenograft model. These findings offer new avenues for the development of therapeutic strategies for neuroendocrine cancer and highlight the utility of pyrvinium as a potential treatment for MCC.
• Adams, A. C., Borden, E. S., Macy, A. M., Thomson, N., Cui, H., Gimbel, M. I., Wilson, M. A., Buetow, K. H., Roe, D. J., DiCaudo, D. J., Homsi, J., & Hastings, K. T. (2022). High GILT Expression Is Associated with Improved Survival in Metastatic Melanoma Patients Treated with Immune Checkpoint Inhibition. Cancers, 14(9).
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  Gamma-interferon-inducible lysosomal thiol reductase (GILT) is critical for MHC class II restricted presentation of multiple melanoma antigens. There is variable GILT protein expression in malignant melanocytes in melanoma specimens. High GILT mRNA expression in melanoma specimens is associated with improved overall survival, before the advent of immune checkpoint inhibitors (ICI). However, the association of GILT in metastatic melanoma with survival in patients treated with ICI and the cell type expressing GILT associated with survival have not been determined. Using RNA sequencing datasets, high GILT mRNA expression in metastatic melanoma specimens was associated with improved progression-free and overall survival in patients treated with ICI. A clinical dataset of metastatic melanoma specimens was generated and annotated with clinical information. Positive GILT immunohistochemical staining in antigen presenting cells and melanoma cells was observed in 100% and 65% of metastatic melanoma specimens, respectively. In the subset of patients treated with ICI in the clinical dataset, high GILT protein expression within melanoma cells was associated with improved overall survival. The association of GILT mRNA and protein expression with survival was independent of cancer stage. These studies support that high GILT mRNA expression in bulk tumor samples and high GILT protein expression in melanoma cells is associated with improved survival in ICI-treated patients. These findings support further investigation of GILT as a biomarker to predict the response to ICI.
• Bhasin, N., Roe, D. J., Saboda, K., Journeycake, J., Moreno, V., & Lentz, S. R. (2022). Association of low serum albumin with venous thrombosis in pediatric patients. Thrombosis research, 218, 48-51.
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  The incidence of venous thromboembolism (VTE) in children is increasing, attributed in part to increased utilization of central venous catheters (CVCs). Children with protein losing disorders (PLDs) and low serum albumin may have an increased incidence of thrombosis. We sought to determine the prevalence of PLDs and hypoalbuminemia at the time of diagnosis of VTE in pediatric patients and its relationship to central venous catheters.
• Bland, V. L., Kindler, J. M., Blew, R. M., Morrill, K. E., Roe, D. J., & Going, S. B. (2022). Visceral adipose tissue and cardiometabolic risk factors in young Hispanic and non-Hispanic girls. Frontiers in pediatrics, 10, 892206.
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  Risk factors for cardiometabolic diseases (e.g., type 2 diabetes, cardiovascular disease) can begin developing in childhood. Elevated body mass index (BMI) is associated with greater likelihood of developing such diseases; however, this relationship varies by race and ethnicity. Notably, Hispanics tend to have high rates of obesity and are disproportionately affected by type 2 diabetes. We aimed to determine if visceral adiposes tissue (VAT) is associated with cardiometabolic risk factors (i.e., triglycerides, cholesterol, insulin resistance, C-reactive protein, and blood pressure), independent of BMI percentile, in a sample of primarily Hispanic adolescent girls.
• Bland, V. L., Klimentidis, Y. C., Bea, J. W., Roe, D. J., Funk, J. L., & Going, S. B. (2022). Cross-sectional associations between adipose tissue depots and areal bone mineral density in the UK Biobank imaging study. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 33(2), 391-402.
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  The relationship between obesity and osteoporosis is poorly understood. In this study, we assessed the association between adiposity and bone. The fat-bone relationship was dependent on sex, body mass index classification, and menopausal status. Results highlight the importance of accounting for direct measures of adiposity (beyond BMI) and menopause status.
• Hingle, M., Blew, R., James, K., Mockbee, J., Palmer, K. N., Roe, D. J., Saboda, K., Shaibi, G. Q., Whitlatch, S., & Marrero, D. (2022). Feasibility and Acceptability of a Type 2 Diabetes Prevention Intervention for Mothers and Children at a Federally Qualified Healthcare Center. Journal of primary care & community health, 12, 21501327211057643.
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  Maternal obesity and gestational diabetes mellitus (GDM) contribute to increased risk for type 2 diabetes mellitus (T2DM) among both mothers and their offspring. Randomized trials demonstrated T2DM risk reduction in adults following lifestyle behavior change and modest weight loss; the evidence base for at-risk children remains limited.
• Katsanis, E., Stea, B., Kovacs, K., Truscott, L., Husnain, M., Khurana, S., Roe, D. J., & Simpson, R. J. (2022). Feasibility and Efficacy of Partially Replacing Post-Transplantation Cyclophosphamide with Bendamustine in Pediatric and Young Adult Patients Undergoing Haploidentical Bone Marrow Transplantation. Transplantation and cellular therapy, 28(7), 390.e1-390.e10.
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  Post-transplantation cyclophosphamide (PT-CY) is the most widely applied graft-versus-host disease (GVHD) prophylaxis regimen in T-cell replete haploidentical bone marrow transplantation (haplo-BMT). Although PT-CY has met with great success in the haplo-BMT arena by suppressing GVHD, patients without acute GVHD have high relapse rates. One strategy to reduce relapse rates being explored by others is a dosage reduction of PT-CY. We have taken a different approach in evaluating whether partially replacing PT-CY with post-transplantation bendamustine (PT-BEN) would be advantageous, an idea based on our preclinical research identifying several beneficial immunomodulatory properties of BEN. We therefore initiated and completed a Phase Ia trial to evaluate the progressive substitution of PT-CY with PT-BEN (ClinicalTrials.gov identifier NCT02996773). We compared outcomes between 13 patients with high-risk hematologic malignancies who received PT-CY/BEN and 31 contemporaneous haplo-BMT recipients treated with the same myeloablative conditioning regimens but receiving only PT-CY. We found that partial replacement of PT-CY with PT-BEN (PT-CY/BEN) on day +4 was well tolerated and associated with significantly earlier trilineage engraftment. We also report favorable trends toward significant improvements on univariate and multivariate analyses with PT-CY/BEN compared with PT-CY with respect to rates of chronic GVHD (hazard ratio [HR], .08; 95% confidence interval [CI], .005 to 1.11; P = .06), and GVHD-free relapse-free survival (GRFS) (HR, .22; 95% CI, .05 to .86; P = .039). Our human trial has now transitioned to Phase Ib, which will further evaluate the safety and potential benefits of PT-CY/BEN. Herein we also expand our pediatric, adolescent, and young adult experience to 31 patients, demonstrating overall survival, progression-free survival, and GRFS at 3 years of 85.6%, 76.1%, and 58.2%, respectively, in a largely racial/ethnic minority cohort. PT-CY/BEN appears to be a promising treatment option that requires further evaluation.
• Langston, M. E., Brown, H. E., Lynch, C. F., Roe, D. J., & Dennis, L. K. (2022). Ambient UVR and Environmental Arsenic Exposure in Relation to Cutaneous Melanoma in Iowa. International journal of environmental research and public health, 19(3).
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  Intermittent sun exposure is the major environmental risk factor for cutaneous melanoma (CM). Cumulative sun exposure and other environmental agents, such as environmental arsenic exposure, have not shown consistent associations. Ambient ultraviolet radiation (UVR) was used to measure individual total sun exposure as this is thought to be less prone to misclassification and recall bias. Data were analyzed from 1096 CM cases and 1033 controls in the Iowa Study of Skin Cancer and Its Causes, a population-based, case-control study. Self-reported residential histories were linked to satellite-derived ambient UVR, spatially derived environmental soil arsenic concentration, and drinking water arsenic concentrations. In men and women, ambient UVR during childhood and adolescence was not associated with CM but was positively associated during adulthood. Lifetime ambient UVR was positively associated with CM in men (OR for highest vs. lowest quartile: 6.09, 95% confidence interval (CI) 2.21-16.8), but this association was not as strong among women (OR for highest vs. lowest quartile: 2.15, 95% CI 0.84-5.54). No association was detected for environmental soil or drinking water arsenic concentrations and CM. Our findings suggest that lifetime and adulthood sun exposures may be important risk factors for CM.
• Lorentzen, G. M., Łaniewski, P., Cui, H., Roe, D. J., Mourad, J., Mahnert, N. D., Farland, L. V., & Herbst-Kralovetz, M. M. (2022). Immunometabolic profiling of cervicovaginal lavages identifies key signatures associated with adenomyosis. iScience, 25(12), 105508.
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  Adenomyosis is a burdensome gynecologic condition that is associated with pelvic pain, dysmenorrhea, and abnormal uterine bleeding, leading to a negative impact on quality of life; and yet is often left undiagnosed. We recruited 108 women undergoing hysterectomy for benign gynecologic conditions and collected non-invasive cervicovaginal lavage samples for immunometabolic profiling. Patients were grouped according to adenomyosis status. We investigated the levels of 72 soluble immune proteins and >900 metabolites using multiplex immunoassays and an untargeted global metabolomics platform. There were statistically significant alterations in the levels of several immune proteins and a large quantity of metabolites, particularly cytokines related to type II immunity and amino acids, respectively. Enrichment analysis revealed that pyrimidine metabolism, carnitine synthesis, and histidine/histamine metabolism were significantly upregulated pathways in adenomyosis. This study demonstrates utility of non-invasive sampling combined with immunometabolic profiling for adenomyosis detection and a greater pathophysiological understanding of this enigmatic condition.
• Martinez, J. A., Taljanovic, M. S., Nuncio Zuniga, A. A., Wertheim, B. C., Roe, D. J., Ehsani, S., Jiralerspong, S., Segar, J., & Chalasani, P. (2022). Feasibility Trial to Evaluate Tendon Stiffness Obtained from Shear Wave Elastography Imaging as a Biomarker of Aromatase Inhibitor-Induced Arthralgias. Journal of clinical medicine, 11(4).
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  Aromatase inhibitor-induced arthralgia (AIA) comprises significant, activity-limiting musculoskeletal symptoms, including joint pain, myalgia, and joint stiffness. We conducted a prospective feasibility study in postmenopausal women diagnosed with early-stage (0-3) hormone receptor positive (HR+) breast cancer who were candidates for treatment with adjuvant AI therapy ( = 16). Tendons of the hands and wrists and the median nerve were imaged using gray-scale and power Doppler ultrasound (US) and US SWE. Arthralgia symptoms were evaluated using the Breast Cancer Prevention Trial (BCPT) Symptom Checklist musculoskeletal subscale (MS) and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain and stiffness subscales. At baseline, there were significant differences in the SW velocities of tendons between dominant and nondominant hands. Increased velocity in 2 of 6 tendons and the median nerve was associated with greater pain at baseline, whereas slower velocity of the extensor digitorum tendon (suggesting decreased stiffness) was associated with a higher WOMAC stiffness score. Increased SW velocity (suggestive of increased stiffness) at baseline in the abductor pollicis longus tendon was associated with a worsening of all three pain and stiffness measures by 6 months. Future studies should evaluate SWE scores related to AIA outcomes in a larger sample size.
• Martinez, J. A., Wertheim, B. C., Roe, D. J., Chalasani, P., Cohen, J., Baer, L., Chow, H. S., Stopeck, A. T., & Thompson, P. A. (2022). Sulindac Improves Stiffness and Quality of Life in Women Taking Aromatase Inhibitors for Breast Cancer. Breast cancer research and treatment, 192(1), 113-122.
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  To examine benefit of sulindac for relief of musculoskeletal symptoms (MSS) in patients stable on aromatase inhibitors (AIs).
• Nakamura, K., Zhu, Z., Roy, S., Jun, E., Han, H., Munoz, R. M., Nishiwada, S., Sharma, G., Cridebring, D., Zenhausern, F., Kim, S., Roe, D. J., Darabi, S., Han, I. W., Evans, D. B., Yamada, S., Demeure, M. J., Becerra, C., Celinski, S. A., , Borazanci, E., et al. (2022). An Exosome-based Transcriptomic Signature for Noninvasive, Early Detection of Patients With Pancreatic Ductal Adenocarcinoma: A Multicenter Cohort Study. Gastroenterology, 163(5), 1252-1266.e2.
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  Pancreatic ductal adenocarcinoma (PDAC) incidence is rising worldwide, and most patients present with an unresectable disease at initial diagnosis. Measurement of carbohydrate antigen 19-9 (CA19-9) levels lacks adequate sensitivity and specificity for early detection; hence, there is an unmet need to develop alternate molecular diagnostic biomarkers for PDAC. Emerging evidence suggests that tumor-derived exosomal cargo, particularly micro RNAs (miRNAs), offer an attractive platform for the development of cancer-specific biomarkers. Herein, genomewide profiling in blood specimens was performed to develop an exosome-based transcriptomic signature for noninvasive and early detection of PDAC.
• Obeng-Kusi, M., Erstad, B., Roe, D. J., & Abraham, I. (2022). Comparative value-based pricing of an Ebola vaccine in resource-constrained countries based on cost-effectiveness analysis. Journal of medical economics, 25(1), 894-902.
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  Pricing, affordability, and access are important deliberations around infectious disease interventions. Determining a fair price that not only incentivizes development but ensures value and access for patients is critical given the increasing global health crisis. Using Ebola virus disease (EVD) as an exemplar, we aim to elucidate the estimation of a jurisdiction-specific value-based price (VBP) for a vaccine package and to consider how prices compare across selected countries that have experienced EVD outbreaks.
• Short, E., Kohler, L. N., Taren, D., Gonzalez, R., Roe, D. J., & Hingle, M. (2022). Diet Quality Following Food Pantry Visit Differs by Ethnicity. Journal of hunger & environmental nutrition, 17(1), 69-84.
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  Food insecurity is associated with poor diet quality and increased diet-related disease risk. Food pantry clients (n=194) completed one 24-hour dietary recall and the Healthy Eating Index-2015 was used to evaluate diet quality. Differences in diet quality relative to participants' last food pantry visit and self-reported ethnicity were evaluated using two-way ANOVA. Food pantry visits within 1-4 days compared to ≥5 days were associated with higher diet quality in non-Hispanics (=0.01) but diet quality remained the same in Hispanics. Interventions to improve diet quality in food pantry users must consider potential ethnic differences when program planning.
• Vrba, L., Futscher, B. W., Oshiro, M., Watts, G. S., Menashi, E., Hu, C., Hammad, H., Pennington, D. R., Golconda, U., Gavini, H., Roe, D. J., Shroff, R. T., & Nelson, M. A. (2022). Liquid biopsy, using a novel DNA methylation signature, distinguishes pancreatic adenocarcinoma from benign pancreatic disease. Clinical epigenetics, 14(1), 28.
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  We tested the ability of a novel DNA methylation biomarker set to distinguish metastatic pancreatic cancer cases from benign pancreatic cyst patients and to monitor tumor dynamics using quantitative DNA methylation analysis of cell-free DNA (cfDNA) from blood samples. The biomarkers were able to distinguish malignant cases from benign disease with high sensitivity and specificity (AUC = 0.999). Furthermore, the biomarkers detected a consistent decline in tumor-derived cfDNA in samples from patients undergoing chemotherapy. The study indicates that our liquid biopsy assay could be useful for management of pancreatic cancer patients.
• Łaniewski, P., Cui, H., Mahnert, N. D., Mourad, J., Borst, M. P., Willmott, L., Chase, D. M., Roe, D. J., & Herbst-Kralovetz, M. M. (2022). Protein biomarkers in cervicovaginal lavages for detection of endometrial cancer. Biomarker research, 10(1), 88.
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  Rates of endometrial cancer (EC) are increasing. For a definitive diagnosis, women undergo various time-consuming and painful medical procedures, such as endometrial biopsy with or without hysteroscopy, and dilation and curettage, which may create a barrier to early detection and treatment, particularly for women with inadequate healthcare access. Thus, there is a need to develop robust EC diagnostics based on non- or minimally-invasive sampling. The objective of this study was to quantify a broad range of immuno-oncology proteins in cervicovaginal lavage (CVL) samples and investigate these proteins as predictive diagnostic biomarkers for EC.
• Adams, A. C., Macy, A. M., Saboda, K., Dickinson, S. E., Glembocki, D. J., Roe, D. J., & Hastings, K. T. (2021). Solar Simulated Light Induces Cutaneous Squamous Cell Carcinoma in Inbred Mice: A Clinically Relevant Model to Investigate T-Cell Responses. The Journal of investigative dermatology, 141(12), 2990-2993.e6.
• Bland, V. L., Bea, J. W., Blew, R. M., Roe, D. J., Lee, V. R., Funk, J. L., & Going, S. B. (2021). Influence of Changes in Soft Tissue Composition on Changes in Bone Strength in Peripubertal Girls: The STAR Longitudinal Study. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 36(1), 123-132.
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  Obesity and osteoporosis remain two major public health concerns. Soft tissue composition and bone are interrelated; however, it is still not well understood how changes in adiposity during adolescence affect bone development. The aim of this study was to assess how changes in DXA-derived total body lean mass (TBLM) and total body fat mass (TBFM) associate with 2-year changes in bone outcomes at the 20% femur, 66% tibia, 66% radius, and 4% tibia, as measured by pQCT, during the years surrounding the onset of menarche in a cohort of 9- to 12-year-old (baseline) adolescent girls (70% Hispanic). From baseline to 2-year follow-up, girls showed statistically significant increases in all bone outcomes, except radial endosteal circumference. In separate linear regression models, change in TBLM and change in TBFM were both positively associated with 2-year changes in bone outcomes at all measured bone sites, after controlling for relevant covariates. However, when change in TBLM and change in TBFM were included in the same model, change in TBLM was the predominant predictor of bone outcomes, explaining 4% to 14% of the variance in bone strength outcomes. Change in TBFM remained a positive predictor of tibia polar strength strain index (SSI (2% variance explained). A significant interaction between change in TBFM and menarcheal status was identified at the radius for SSI and indicated that greater gains in TBFM were beneficial for SSI in girls that were premenarcheal at baseline but detrimental for girls who were postmenarcheal at baseline. The overall findings suggest that changes in TBLM during the peripubertal years have a greater influence on bone outcomes than changes in TBFM. While gains in TBFM might benefit the weight bearing 66% tibia, greater gains in TBFM may be detrimental to bone development at the non-weight bearing 66% radius after the onset of menarche. © 2020 American Society for Bone and Mineral Research (ASBMR).
• Bland, V., Bea, J. W., Blew, R., Roe, D., Lee, V. L., Funk, J. L., & Going, S. B. (2021). Influence of Changes in Soft Tissue Composition on Changes in Bone Strength in Peripubertal Girls: The STAR Longitudinal Study. Journal of Bone and Mineral Research, 36, 123-132. doi:10.1002/jbmr.4168
• Gachupin, F. C., Caston, E., Chavez, C., Bernal, J., Cager, P., Harris, D., John, T., Remitera, J., Garcia, C. A., Romero, V. M., Gchachu, K. E., Gchachu, C. R., Garcia, K., Gchachu, V., Gchachu, B. M., Rens, E., Slowtalker, J., Blew, R., Tracy, K., , Figueroa, T., et al. (2021). Primary Disease Prevention for Southwest American Indian Families During the COVID-19 Pandemic: Camp in a Box. Frontiers in sociology, 6, 611972.
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  The goal of the American Indian Youth Wellness Camp in a Box was to engage, educate and empower families to improve their health and overall well-being during the COVID-19 pandemic. Camp in a Box was a 9-week program, inclusive of a 1-week intensive camp component followed by an 8-week booster component with content focused on nutrition, mental health and physical activity education. The Camp in a Box is a Tribal/Urban Indian-University partnership, and materials were developed to replace an existing weeklong residential camp and to comply with social distancing guidelines. Fourteen American Indian families from Tribal/Urban Indian communities in the southwestern United States participated (36 children aged 2-18 years; 32 adults). The intensive camp week included daily materials for families to complete together, Monday through Friday. Materials were provided for approximately 4 h of activities per day. The booster sessions began after camp week and included approximately 4 h of supplementary activities designed to be completed at any time most convenient for the family over the course of the week. Activities were designed to encourage interaction among family members with materials and supplies for parents and youth to participate. Self-reported outcomes suggested that families changed their eating habits to include more vegetables, less sweets and junk food. Parents reported an increase in family physical activity and that the activities brought the family closer together. Our Camp in a Box program was feasible and well-received until school began. During camp week, 100% of recruited families participated; at Booster Week 8, ten families (71%) remained enrolled and active. Camp in a Box is a feasible alternative to residential camps for promotion of health behaviors associated with metabolic disease prevention among American Indian families. In contrast to residential camps for youth, Camp in a Box offers an opportunity to engage the entire family in health promotion activities.
• Goyal, U. D., Mehta, P. P., Samreth, S., Gloss, J., Cui, H., Roe, D., & Dougherty, S. (2021). Reproducibility of vaginal immobilization balloons overnight for cervical cancer brachytherapy. Journal of contemporary brachytherapy, 13(3), 280-285.
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  The use of vaginal immobilization balloons placed into the vagina for immobilization of tandem and ovoid (T+O) applicator during high-dose-rate (HDR) brachytherapy delivery has been used at our institution, and seems to have improved our patient comfort, decreased procedure time, and minimized applicator misplacement. We aimed to show that these balloons, while originally marketed for single-day use, are safe and maintain applicator positioning/dosimetry when left overnight for treatment delivery on sequential days.
• Marrero, D. G., Blew, R. M., Palmer, K. N., James, K., Roe, D. J., & Hingle, M. D. (2021). Rationale and design of a type 2 diabetes prevention intervention for at-risk mothers and children at a Federally Qualified Healthcare Center: EPIC El Rio Families Study Protocol. BMC public health, 21(1), 346.
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  Exposure to gestational diabetes mellitus (GDM) is associated with increased risk for type 2 diabetes (T2DM) in mothers, and poor cardiovascular health among offspring. Identifying effective methods to mitigate T2DM risk has the potential to improve health outcomes for mothers with a history of GDM and their children. The goal of the EPIC El Rio Families Study is to implement and evaluate the effects of a 13-week behavioral lifestyle intervention on T2DM risk factors in at-risk mothers and their 8- to 12-year-old children. We describe herein the rationale for our specific approach, the adaption of the DPP-based curriculum for delivery to patients of a Federally Qualified Health Center (FQHC), and the study design and methodology.
• Martinez, J. A., Taljanovic, M. S., Witte, R. S., Nuncio Zuniga, A. A., Wertheim, B. C., Kwoh, C. K., Goldstein, B. A., Roe, D. J., & Chalasani, P. (2021). Shear wave elastography detects novel imaging biomarkers of aromatase inhibitor-induced joint pain: a pilot study. Journal of ultrasonography, 21(84), 1-6.
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  To determine whether differences in joint and tendon stiffness as measured by ultrasound shear wave elastography are present in breast cancer patients with aromatase inhibitor-associated arthralgias compared to age-comparable healthy control women.
• Obeng-Kusi, M., Habila, M. A., Roe, D. J., Erstad, B., & Abraham, I. (2021). Economic evaluation using dynamic transition modeling of ebola virus vaccination in lower-and-middle-income countries. Journal of medical economics, 24(sup1), 1-13.
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  With the increasing occurrence of infectious diseases in lower-and-middle-income countries (LMICs), emergency preparedness is essential for rapid response and mitigation. Economic evaluations of mitigation technologies and strategies have been recommended for inclusion in emergency preparedness plans. We aimed to perform an economic evaluation using dynamic transition modeling of ebola virus disease (EVD) vaccination in a hypothetical community of 1,000 persons in the Democratic Republic of Congo (DRC).
• Ranjbar, N. E., Hingle, M. D., Thomson, C. A., Figueroa, T., Tracy, K., Blew, R., Slowtalker, J., Rens, E., Gchachu, B., Garcia, K., Gchachu, V., Gchachu, C., Gchachu, K., Romero, V., Garcia, C. A., Remitera, J., John, T., Harris, D., Cager, P., , Bernal, J., et al. (2021). Primary Disease Prevention for Southwest American Indian Families During the COVID-19 Pandemic: Camp in a Box.. Frontiers.
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  Gachupin FC, Caston E, Chavez C, Bernal J, Cager P, Harris D, John T, Remitera J, Garcia CA, Romero V, Gchachu KE, Gchachu CR, Gchachu V, Garcia K, Gchachu BM, Rens E, Slowtalker J, Blew R, Tracy K, Figueroa T, Thomson CA, Ranjbar N, Hingle M, O’Connor, Roe D, Grant V, Swick S, Joe JR. Primary Disease Prevention for Southwest American Indian Families During the COVID-19 Pandemic: Camp in a Box. Frontiers.
• Tapia, E., Villa-Guillen, D. E., Chalasani, P., Centuori, S., Roe, D. J., Guillen-Rodriguez, J., Huang, C., Galons, J. P., Thomson, C. A., Altbach, M., Trujillo, J., Pinto, L., Martinez, J. A., Algotar, A. M., & Chow, H. S. (2021). A randomized controlled trial of metformin in women with components of metabolic syndrome: intervention feasibility and effects on adiposity and breast density. Breast cancer research and treatment, 190(1), 69-78.
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  Obesity is a known risk factor for post-menopausal breast cancer and may increase risk for triple negative breast cancer in premenopausal women. Intervention strategies are clearly needed to reduce obesity-associated breast cancer risk.
• Thompson, P. A., Huang, C., Yang, J., Wertheim, B. C., Roe, D., Zhang, X., Ding, J., Chalasani, P., Preece, C., Martinez, J., Chow, H. S., & Stopeck, A. T. (2021). Sulindac, a Nonselective NSAID, Reduces Breast Density in Postmenopausal Women with Breast Cancer Treated with Aromatase Inhibitors. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(20), 5660-5668.
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  To evaluate the effect of sulindac, a nonselective anti-inflammatory drug (NSAID), for activity to reduce breast density (BD), a risk factor for breast cancer.
• AM, L., Henry, N., Roe, D., Rodriguez, C., Romero, R., & Ingram, M. (2020). Evaluation of the Impact of School Garden Exposure on Youth Outlook and Behaviors toward Vegetables in Southern Arizona.. J Sch Health. doi:10.1111/josh.12905
• Bland, V. L., Bea, J. W., Roe, D. J., Lee, V. R., Blew, R. M., & Going, S. B. (2020). Physical activity, sedentary time, and longitudinal bone strength in adolescent girls. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 31(10), 1943-1954.
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  The association between baseline physical activity and sedentary time with 2-year longitudinal bone strength was evaluated. The effect of physical activity on bone depended on maturity status. Sedentary time did not negatively impact bone outcomes, regardless of maturity. Maturity should be considered when developing exercise interventions to improve bone outcomes.
• Chalasani, P., Taljanovic, M., Segar, J., Farr, K., Win, H., Wertheim, B. C., Chu-Pilli, M., Ehsani, S., Roe, D. J., & Gimber, L. (2020). Diffuse tensor imaging of lower extremities: a novel MR imaging technique for chemotherapy-induced peripheral neuropathy. Breast cancer research and treatment, 184(3), 771-778.
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  Chemotherapy-induced peripheral neuropathy (CIPN) is caused by drug-induced damage to the axons which is not detected easily due to lack of reliable, clinically applicable modalities. Diffuse tensor imaging (DTI) allows for quantitative measurements of fractional anisotropy (FA) and apparent diffusion coefficient (ADC), which have been shown to detect nerve injury by Magnetic Resonance Imaging (MRI).
• Goyal, U., Saboda, K., Roe, D., & Gonzalez, V. J. (2020). Prone Positioning With Deep Inspiration Breath Hold for Left Breast Radiotherapy. Clinical breast cancer.
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  With advances in treatment, outcomes for early-stage breast cancer are improving. We investigated the combination of prone position and deep inspiration breath hold to decrease cardiac doses for left-sided breast radiotherapy.
• Hingle, M. D., Roe, D., Gonzalez, R., Taren, D. L., Kohler, L. N., & Short, E. (2020). Diet Quality Following Food Pantry Visit Differs by Ethnicity. Journal of Hunger and Environmental Nutrition. doi:https://doi.org/10.1080/19320248.2020.1860849
• Katsanis, E., Maher, K., Roe, D. J., & Simpson, R. J. (2020). Progressive substitution of posttransplant cyclophosphamide with bendamustine: A phase I study in haploidentical bone marrow transplantation. eJHaem, 1(1), 286-292.
• Lohr, A. M., Henry, N., Roe, D., Rodriguez, C., Romero, R., & Ingram, M. (2020). Evaluation of the Impact of School Garden Exposure on Youth Outlook and Behaviors toward Vegetables in Southern Arizona. The Journal of school health, 90(7), 572-581.
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  Our objective was to evaluate the effect of garden-based learning on outlook and behaviors toward vegetables among primarily Latinx students. An educational strategy, garden-based learning is a teaching tool that complements other disciplines.
• Miller, M. L., Roe, D. J., Hu, C., & Bell, M. L. (2020). Power difference in a χ test vs generalized linear mixed model in the presence of missing data - a simulation study. BMC medical research methodology, 20(1), 50.
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  Longitudinal randomized controlled trials (RCTs) often aim to test and measure the effect of treatment between arms at a single time point. A two-sample χ test is a common statistical approach when outcome data are binary. However, only complete outcomes are used in the analysis. Missing responses are common in longitudinal RCTs and by only analyzing complete data, power may be reduced and estimates could be biased. Generalized linear mixed models (GLMM) with a random intercept can be used to test and estimate the treatment effect, which may increase power and reduce bias.
• Nisson, P. L., Meybodi, A. T., Secomb, T. W., Berger, G. K., Roe, D. J., & Lawton, M. T. (2020). Patients Taking Antithrombotic Medications Less Frequently Present with Ruptured Aneurysms. World neurosurgery, 136, e132-140. doi:doi: 10.1016/jwneu2019.12:045
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  Conflicting findings exist on the protective role of aspirin against aneurysmal subarachnoid hemorrhage (SAH). In this retrospective analysis, we compare the risk of SAH at presentation between patients treated microsurgically who were regularly taking an antithrombotic medication versus those who were not.
• Nuno, V. L., Wertheim, B., Murphy, B., Wahl, R. A., & Roe, D. (2020). Does the Nurtured Heart Approach improve ADHD behaviors in children? A randomized unblinded controlled trial.. Family Process.
• Recio-Boiles, A., Vondrak, J., Veeravelli, S., Mancuso, J. J., Saboda, K., Roe, D. J., Riaz, I. B., Scott, A. J., Elquza, E., McBride, A., & Babiker, H. M. (2020). Analyzing outcomes of neoadjuvant and adjuvant treatment for borderline-resectable pancreatic adenocarcinoma in the perioperative period at an academic institution. Annals of pancreatic cancer, 3.
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  Only 15-20% of pancreatic ductal adenocarcinoma (PDAC) patients are upfront surgical candidates at presentation, and for this cohort of patients, the 5-year survival is a mere 20% despite adjuvant therapy. Previous data indicate that in clinical practice most of these cases are "borderline-resectable," and there is currently no mature data on perioperative treatment.
• Roe, D. J., Roe, D. J., Roe, D. J., Wahl, R. A., Wahl, R. A., Wahl, R. A., Glasser, H. N., Glasser, H. N., Glasser, H. N., Murphy, B. S., Murphy, B. S., Murphy, B. S., Wertheim, B. C., Wertheim, B. C., Wertheim, B. C., Nuno, V. L., Nuno, V. L., & Nuno, V. L. (2020). The Online Nurtured Heart Approach to Parenting: A Randomized Study to Improve ADHD Behaviors in Children Ages 6-8.. Ethical Human Psychology and Psychiatry,, 20(1), 169 - 186. doi:http://dx.doi.org/10.1891/EHPP-D-20-00013
• Łaniewski, P., Cui, H., Roe, D. J., Chase, D. M., & Herbst-Kralovetz, M. M. (2020). Vaginal microbiota, genital inflammation, and neoplasia impact immune checkpoint protein profiles in the cervicovaginal microenvironment. NPJ precision oncology, 4, 22.
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  Emerging evidence suggests that the vaginal microbiota play a role in HPV persistence and cervical neoplasia development and progression. Here we examine a broad range of immune checkpoint proteins in the cervicovaginal microenvironment across cervical carcinogenesis and explore relationships among these key immunoregulatory proteins, the microbiota composition, and genital inflammation. First, we demonstrate that immune checkpoint molecules can be measured in cervicovaginal lavages. Secondly, we identify CD40, CD27, and TIM-3 to specifically discriminate cervical cancer from other groups and CD40, CD28, and TLR2 to positively correlate to genital inflammation. Finally, PD-L1 and LAG-3 levels negatively, whereas TLR2 positively correlate to health-associated dominance. Overall, our study identifies immune checkpoint signatures associated with cervical neoplasm and illuminates the multifaceted microbiota-host immunity network in the local microenvironment. This study provides a foundation for future mechanistic studies and highlights the utility of cervicovaginal lavage profiling for predicting and monitoring response to cancer therapy.
• Buetow, K. H., Meador, L. R., Menon, H., Lu, Y. K., Brill, J., Cui, H., Roe, D. J., DiCaudo, D. J., & Hastings, K. T. (2019). High GILT Expression and an Active and Intact MHC Class II Antigen Presentation Pathway Are Associated with Improved Survival in Melanoma. Journal of immunology (Baltimore, Md. : 1950), 203(10), 2577-2587.
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  The MHC class I Ag presentation pathway in melanoma cells has a well-established role in immune-mediated destruction of tumors. However, the clinical significance of the MHC class II Ag presentation pathway in melanoma cells is less clear. In Ag-presenting cells, IFN-γ-inducible lysosomal thiol reductase (GILT) is critical for MHC class II-restricted presentation of multiple melanoma Ags. Although not expressed in benign melanocytes of nevi, GILT and MHC class II expression is induced in malignant melanocytes in a portion of melanoma specimens. Analysis of The Cancer Genome Atlas cutaneous melanoma data set showed that high GILT mRNA expression was associated with improved overall survival. Expression of IFN-γ, TNF-α, and IL-1β was positively associated with GILT expression in melanoma specimens. These cytokines were capable of inducing GILT expression in human melanoma cells in vitro. GILT protein expression in melanocytes was induced in halo nevi, which are nevi undergoing immune-mediated regression, and is consistent with the association of GILT expression with improved survival in melanoma. To explore potential mechanisms of GILT's association with patient outcome, we investigated pathways related to GILT function and expression. In contrast to healthy skin specimens, in which the MHC class II pathway was nearly uniformly expressed and intact, there was substantial variation in the MHC class II pathway in the The Cancer Genome Atlas melanoma specimens. Both an active and intact MHC class II pathway were associated with improved overall survival in melanoma. These studies support a role for GILT and the MHC class II Ag presentation pathway in melanoma outcome.
• Hetherington-Rauth, M., Bea, J. W., Blew, R. M., Funk, J. L., Lee, V. R., Roe, D. J., Sardinha, L. B., & Going, S. B. (2019). Relationship of cardiometabolic risk biomarkers with DXA and pQCT bone health outcomes in young girls. BONE, 120, 452-458.
• Hingle, M. D., Turner, T., Going, S., Ussery, C., Roe, D. J., Saboda, K., Kutob, R., & Stump, C. (2019). Feasibility of a family-focused YMCA-based diabetes prevention program in youth: The E.P.I.C. Kids (Encourage, Practice, and Inspire Change) Study. Preventive medicine reports, 14, 100840.
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  Efficacious lifestyle modification programs for children at risk of type 2 diabetes (T2D) have not been well established outside of clinical settings. In this study, the feasibility of a family-focused, YMCA-based prevention program for children at risk of T2D was evaluated between September 2015 and July 2016 in Tucson, Arizona. A 12-week YMCA-led lifestyle intervention was adapted for 9-12-year-old children and their families to encourage healthy eating, physical activity, and supportive home environments. Two YMCA locations were randomized to offer either a face-to-face lifestyle coach-led intervention or an alternating face-to-face and digitally-delivered intervention. Program feasibility and preliminary effects on child anthropometric and behavioral outcomes were assessed at baseline and post-intervention. Changes were assessed using linear regression combining delivery formats, with adjustment for clustering of participants within site/format. Forty-eight children (10.9 ± 1.2 years old; 45% female; 40% Hispanic; 43% White; 87% obese) and their parents enrolled, and 36 (75%) completed 12-week measures. Weekly program attendance averaged 61%. Participants and coaches highly rated program content and engagement strategies. Statistically significant changes in child BMI-z score (-0.05,   0.03) and family food and physical activity environment (+5.5% family nutrition and physical activity score,  = 0.01) were observed. A YMCA-led family-focused T2D intervention was feasible for the YMCA and participants and effects on child weight, behavior, and the home environment warranted further investigation.
• Ilhan, Z. E., Łaniewski, P., Thomas, N., Roe, D. J., Chase, D. M., & Herbst-Kralovetz, M. M. (2019). Deciphering the complex interplay between microbiota, HPV, inflammation and cancer through cervicovaginal metabolic profiling. EBioMedicine, 44, 675-690.
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  Dysbiotic vaginal microbiota have been implicated as contributors to persistent HPV-mediated cervical carcinogenesis and genital inflammation with mechanisms unknown. Given that cancer is a metabolic disease, metabolic profiling of the cervicovaginal microenvironment has the potential to reveal the functional interplay between the host and microbes in HPV persistence and progression to cancer.
• Nisson, P. L., Fard, S. A., Walter, C. M., Johnstone, C. M., Mooney, M. A., Tayebi Meybodi, A., Lang, M., Kim, H., Jahnke, H., Roe, D. J., Dumont, T. M., Lemole, G. M., Spetzler, R. F., & Lawton, M. T. (2019). A novel proposed grading system for cerebellar arteriovenous malformations. Journal of neurosurgery, 1-11.
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  OBJECTIVEThe objective of this study was to evaluate the existing Spetzler-Martin (SM), Spetzler-Ponce (SP), and Lawton-Young (LY) grading systems for cerebellar arteriovenous malformations (AVMs) and to propose a new grading system to estimate the risks associated with these lesions.METHODSData for patients with cerebellar AVMs treated microsurgically in two tertiary medical centers were retrospectively reviewed. Data from patients at institution 1 were collected from September 1999 to February 2013, and at institution 2 from October 2008 to October 2015. Patient outcomes were classified as favorable (modified Rankin Scale [mRS] score 0-2) or poor (mRS score 3-6) at the time of discharge. Using chi-square and logistic regression analysis, variables associated with poor outcomes were assigned risk points to design the proposed grading system. The proposed system included neurological status prior to treatment (poor, +2 points), emergency surgery (+1 point), age > 60 years (+1 point), and deep venous drainage (deep, +1 point). Risk point totals of 0-1 comprised grade 1, 2-3 grade 2, and 4-5 grade 3.RESULTSA total of 125 cerebellar AVMs of 1328 brain AVMs were reviewed in 125 patients, 120 of which were treated microsurgically and included in the study. With our proposed grading system, we found poor outcomes differed significantly between each grade (p < 0.001), while with the SM, SP, and LY grading systems they did not (p = 0.22, p = 0.25, and p = 1, respectively). Logistic regression revealed grade 2 had 3.3 times the risk of experiencing a poor outcome (p = 0.008), while grade 3 had 9.9 times the risk (p < 0.001). The proposed grading system demonstrated a superior level of predictive accuracy (area under the receiver operating characteristic curve [AUROC] of 0.72) compared with the SM, SP, and LY grading systems (AUROC of 0.61, 0.57, and 0.51, respectively).CONCLUSIONSThe authors propose a novel grading system for cerebellar AVMs based on emergency surgery, venous drainage, preoperative neurological status, and age that provides a superior prognostication power than the formerly proposed SM, SP, and LY grading systems. This grading system is clinically predictive of patient outcomes and can be used to better guide vascular neurosurgeons in clinical decision-making.
• Nuño, V. L., Wertheim, B. C., Murphy, B. S., Wahl, R. A., & Roe, D. J. (2019). Testing the efficacy of the Nurtured Heart Approach to reduce ADHD symptoms in children by training parents: Protocol for a randomized controlled trial. Contemporary clinical trials communications, 13, 100312.
• Pearson, T., Caporaso, J. G., Yellowhair, M., Bokulich, N. A., Padi, M., Roe, D. J., Wertheim, B. C., Linhart, M., Martinez, J. A., Bilagody, C., Hornstra, H., Alberts, D. S., Lance, P., & Thompson, P. A. (2019). Effects of ursodeoxycholic acid on the gut microbiome and colorectal adenoma development. Cancer medicine.
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  It has been previously reported that ursodeoxycholic acid (UDCA), a therapeutic bile acid, reduced risk for advanced colorectal adenoma in men but not women. Interactions between the gut microbiome and fecal bile acid composition as a factor in colorectal cancer neoplasia have been postulated but evidence is limited to small cohorts and animal studies. Using banked stool samples collected as part of a phase III randomized clinical trial of UDCA for the prevention of colorectal adenomatous polyps, we compared change in the microbiome composition after a 3-year intervention in a subset of participants randomized to oral UDCA at 8-10 mg/kg of body weight per day (n = 198) or placebo (n = 203). Study participants randomized to UDCA experienced compositional changes in their microbiome that were statistically more similar to other individuals in the UDCA arm than to those in the placebo arm. This reflected a UDCA-associated shift in microbial community composition (P  0.05). These UDCA-associated shifts in microbial community distance metrics from baseline to end-of-study were not associated with risk of any or advanced adenoma (all P > 0.05) in men or women. Separate analyses of microbial networks revealed an overrepresentation of Faecalibacterium prausnitzii in the post-UDCA arm and an inverse relationship between F prausnitzii and Ruminococcus gnavus. In men who received UDCA, the overrepresentation of F prausnitzii and underrepresentation of R gnavus were more prominent in those with no adenoma recurrence at follow-up compared to men with recurrence. This relationship was not observed in women. Daily UDCA use modestly influences the relative abundance of microbial species in stool and affects the microbial network composition with suggestive evidence for sex-specific effects of UDCA on stool microbial community composition as a modifier of colorectal adenoma risk.
• Puleo, J. I., Parker, S. S., Roman, M. R., Watson, A. W., Eliato, K. R., Peng, L., Saboda, K., Roe, D. J., Ros, R., Gertler, F. B., & Mouneimne, G. (2019). Mechanosensing during directed cell migration requires dynamic actin polymerization at focal adhesions. The Journal of cell biology, 218(12), 4215-4235.
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  The mechanical properties of a cell's microenvironment influence many aspects of cellular behavior, including cell migration. Durotaxis, the migration toward increasing matrix stiffness, has been implicated in processes ranging from development to cancer. During durotaxis, mechanical stimulation by matrix rigidity leads to directed migration. Studies suggest that cells sense mechanical stimuli, or mechanosense, through the acto-myosin cytoskeleton at focal adhesions (FAs); however, FA actin cytoskeletal remodeling and its role in mechanosensing are not fully understood. Here, we show that the Ena/VASP family member, Ena/VASP-like (EVL), polymerizes actin at FAs, which promotes cell-matrix adhesion and mechanosensing. Importantly, we show that EVL regulates mechanically directed motility, and that suppression of expression impedes 3D durotactic invasion. We propose a model in which EVL-mediated actin polymerization at FAs promotes mechanosensing and durotaxis by maturing, and thus reinforcing, FAs. These findings establish dynamic FA actin polymerization as a central aspect of mechanosensing and identify EVL as a crucial regulator of this process.
• Recio-Boiles, A., Nallagangula, A., Veeravelli, S., Vondrak, J., Saboda, K., Roe, D., Elquza, E., McBride, A., & Babiker, H. M. (2019). Neutrophil-to-lymphocyte and platelet-to-lymphocyte ratios inversely correlate to clinical and pathologic stage in patients with resectable pancreatic ductal adenocarcinoma. Annals of pancreatic cancer, 2.
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  Post-surgical pathology (SP) staging correlates with long-term survival. Neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) have been shown to predict prognosis and extent of tumor in patients with metastatic pancreatic ductal adenocarcinoma (PDAC). This study aimed to correlate NLR and PLR to radiological clinical staging (CS), carbohydrate antigen (CA) 19-9 tumor marker and SP staging in patients with resectable-PDAC (R-PDAC); and to investigate NLR and PLR as potential markers to guide neoadjuvant therapy.
• Roe, D., Roe, D., Wahl, R. A., Wahl, R. A., Murphy, B. S., Murphy, B. S., Wertheim, B. C., Wertheim, B. C., Nuno, V. L., & Nuno, V. L. (2019). Testing the efficacy of the Nurtured Heart Approach to reduce ADHD symptoms in children by training parents: Protocol for a randomized controlled trial.. Contemporary Clinical Trials Communications, 13, 1-6. doi:https://doi.org/10.1016/j.conctc.2018.100312
• Łaniewski, P., Cui, H., Roe, D. J., Barnes, D., Goulder, A., Monk, B. J., Greenspan, D. L., Chase, D. M., & Herbst-Kralovetz, M. M. (2019). Features of the cervicovaginal microenvironment drive cancer biomarker signatures in patients across cervical carcinogenesis. Scientific reports, 9(1), 7333.
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  Persistent human papillomavirus (HPV) infection is the vital factor driving cervical carcinogenesis; however, other features of the local cervicovaginal microenvironment (CVM) may play a critical role in development of precancerous cervical dysplasia and progression to invasive cervical carcinoma (ICC). Here we investigated relationships between locally secreted cancer biomarkers and features of the local CVM to better understand the complex interplay between host, virus and vaginal microbiota (VMB). We enrolled women with ICC, high- and low-grade squamous intraepithelial lesions, as well as, HPV-positive and healthy HPV-negative controls. A broad range of cancer biomarkers was present in the local CVM and specifically elevated in ICC patients. The majority of cancer biomarkers were positively correlated to other biomarkers and linked to genital inflammation. Several cancer biomarkers were also negatively correlated to Lactobacillus abundance and positively correlated with abnormal vaginal pH. Finally, a hierarchical clustering analysis of cancer biomarkers and immune mediators revealed three patient clusters, which varied in levels of cancer biomarkers, genital inflammation, vaginal pH and VMB composition. Specific cancer biomarkers discriminated patients with features of the CVM, such as high genital inflammation, elevated vaginal pH and dysbiotic non-Lactobacillus-dominant VMB, that have been associated with HPV persistence, dysplasia and progression to ICC.
• Augustus, G. J., Roe, D. J., Jacobs, E. T., Lance, P., & Ellis, N. A. (2018). Is increased colorectal screening effective in preventing distant disease?. PloS one, 13(7), e0200462.
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  Screening in the average risk population for colorectal cancer (CRC) is expected to reduce the incidence of distant (i.e., metastatic) CRCs at least as much as less advanced CRCs. Indeed, since 2000, during which time colonoscopy became widely used as a screening tool, the overall incidence of CRC has been reduced by 29%.
• Bauman, A., Ernst, K., Hayden, M., Roe, D. J., Murray, R., Agawo, M., Munga, S., Schmahl, E., & Taren, D. (2018). Assessing Community Health: An Innovative Tool for Measuring Height and Length. JOURNAL OF TROPICAL PEDIATRICS, 64(2), 146-150.
• Bea, J. W., Funk, J., Hetherington-Rauth, M., Wertheim, B. C., Mosquiera, L., Thuraisingam, R., Lee, V., Blew, R., Lohman, T., Roe, D. J., & Going, S. (2018). Anthropometry Versus Imaging for Prediction of Inflammation Among Hispanic Girls. Obesity (Silver Spring, Md.), 26(10), 1594-1602.
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  This study aimed to compare total and regional estimates of body composition, by direct and indirect techniques, for the optimal prediction of C-reactive protein (CRP) among young (aged 9-12 years) Hispanic girls (N = 232).
• Bedrick, E. J., Vasquez, M., Roe, D., Guerra, S., Billheimer, D. D., Bell, M. L., Hu, C., & Huang, S. (2018). Regularized continuous-time Markov model via elastic net. Biometrics.
• Blohm-Mangone, K., Burkett, N. B., Tahsin, S., Myrdal, P. B., Aodah, A., Ho, B., Janda, J., McComas, M., Saboda, K., Roe, D. J., Dong, Z., Bode, A. M., Petricoin, E. F., Calvert, V. S., Curiel-Lewandrowski, C., Alberts, D. S., Wondrak, G. T., & Dickinson, S. E. (2018). Pharmacological TLR4 antagonism using topical resatorvid blocks solar UV-induced skin tumorigenesis in SKH-1 mice. Cancer prevention research (Philadelphia, Pa.).
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  An urgent need exists for the development of more efficacious molecular strategies targeting non-melanoma skin cancer (NMSC), the most common malignancy worldwide. Inflammatory signaling downstream of Toll-like receptor 4 (TLR4) has been implicated in several forms of tumorigenesis, yet its role in solar UV-induced skin carcinogenesis remains undefined. We have previously shown in keratinocyte cell culture and SKH-1 mouse epidermis that topical application of the specific TLR4 antagonist resatorvid (TAK-242) blocks acute UV-induced AP-1 and NF-κB signaling, associated with downregulation of inflammatory mediators and MAP Kinase phosphorylation. We therefore explored TLR4 as a novel target for chemoprevention of UV-induced NMSC. We selected the clinical TLR4 antagonist resatorvid based upon target specificity, potency, and physico-chemical properties. Here we confirm using ex-vivo permeability assays that topical resatorvid can be effectively delivered to skin, and using in vivo studies that topical resatorvid can block UV-induced AP-1 activation in mouse epidermis. We also report that in a UV-induced skin tumorigenesis model, topical resatorvid displays potent photochemopreventive activity, significantly suppressing tumor area and multiplicity. Tumors harvested from resatorvid-treated mice display reduced activity of UV-associated signaling pathways and a corresponding increase in apoptosis compared to tumors from control animals. Further mechanistic insight on resatorvid-based photochemoprevention was obtained from unsupervised hierarchical clustering analysis of protein readouts via reverse-phase protein microarray revealing a significant attenuation of key UV-induced proteomic changes by resatorvid in chronically treated high-risk SKH-1 skin prior to tumorigenesis. Taken together, our data identify TLR4 as a novel molecular target for topical photochemoprevention of NMSC.
• Bui, D. P., Oren, E., Roe, D. J., Brown, H. E., Harris, R. B., Knight, G. M., Gilman, R. H., & Grandjean, L. (2018). A Case Control Study to Identify Community Venues Associated with Genetically Clustered Multidrug-Resistant Tuberculosis Disease in Lima, Peru. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.
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  The majority of tuberculosis transmission occurs in community settings. The primary aim of this study was to assess the association between exposure to community venues and multidrug-resistant tuberculosis (MDR-TB) disease. The secondary aim was to describe the social networks of MDR-TB cases and controls.
• Ding, J., Stopeck, A. T., Gao, Y., Marron, M. T., Wertheim, B. C., Altbach, M. I., Galons, J. P., Roe, D. J., Wang, F., Maskarinec, G., Thomson, C. A., Thompson, P. A., & Huang, C. (2018). Reproducible automated breast density measure with no ionizing radiation using fat-water decomposition MRI. Journal of magnetic resonance imaging : JMRI, 48(4), 971-981.
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  Increased breast density is a significant independent risk factor for breast cancer, and recent studies show that this risk is modifiable. Hence, breast density measures sensitive to small changes are desired.
• Gilligan, A. M., Alberts, D. S., Roe, D. J., & Skrepnek, G. H. (2018). Death or Debt? National Estimates of Financial Toxicity in Persons with Newly-Diagnosed Cancer. The American journal of medicine, 131(10), 1187-1199.e5.
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  The purpose of this study was to evaluate the impact of cancer upon a patient's net worth and debt in the US.
• Going, S. B., Lohman, T. G., Wheeler, M. D., Lee, V. R., Roe, D., Hingle, M. D., Funk, J. L., Blew, R. M., Bea, J. W., & Hetherington-Rauth, M. C. (2018). Relative contributions of lean and fat mass to bone strength in young Hispanic and non-Hispanic girls. Bone, 113, 144-150. doi:10.1016/j.bone.2018.05.023
• Going, S. B., Roe, D., Funk, J. L., Blew, R., Lee, V., Bea, J. W., & Hetherington-Rauth, M. (2018). Relationship between fat distribution and cardiometabolic risk in preadolescent Hispanic girls. American Journal of Human Biology.
• Going, S. B., Roe, D., Lohman, T. G., Blew, R., Lee, V., Thuraisingam, R., Mosquiera, L., Wertheim, B. C., Hetherington-Rauth, M. C., Funk, J. L., & Bea, J. W. (2018). Anthropometry versus imaging for the prediction of inflammation among Hispanic girls. Obesity (Silver Spring), 26(10), 1594-1602. doi:10.1002/oby.22265
• Hetherington-Rauth, M., Bea, J. W., Blew, R. M., Funk, J. L., Hingle, M. D., Lee, V. R., Roe, D. J., Wheeler, M. D., Lohman, T. G., & Going, S. B. (2018). Relative contributions of lean and fat mass to bone strength in young Hispanic and non-Hispanic girls. Bone, 113, 144-150.
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  With the high prevalence of childhood obesity, especially among Hispanic children, understanding how body weight and its components of lean and fat mass affect bone development is important, given that the amount of bone mineral accrued during childhood can determine osteoporosis risk later in life. The aim of this study was to assess the independent contributions of lean and fat mass on volumetric bone mineral density (vBMD), geometry, and strength in both weight-bearing and non-weight-bearing bones of Hispanic and non-Hispanic girls.
• Hetherington-Rauth, M., Bea, J. W., Blew, R. M., Funk, J. L., Lee, V. R., Roe, D. J., Sardinha, L. B., & Going, S. B. (2018). Relationship of cardiometabolic risk biomarkers with DXA and pQCT bone health outcomes in young girls. Bone, 120, 452-458.
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  Excess weight exerts the positive effect of mechanical loading on bone during development whereas obesity-related metabolic dysfunction may have a detrimental impact. In adults, the presence of metabolic syndrome and type 2 diabetes has been associated with compromised bone density, quality, and strength, and an increased incidence of fractures. The few studies that have investigated the role of cardio-metabolic disease risk biomarkers (CMR) on bone strength in children have given conflicting results. The aim of this study was to assess the combined and independent relationships of cardio-metabolic biomarkers with total body and regional bone parameters in young girls.
• Hetherington-Rauth, M., Bea, J. W., Blew, R. M., Funk, J. L., Lee, V. R., Varadi, T. C., Roe, D. J., Wheeler, M. D., & Going, S. B. (2018). Effect of cardiometabolic risk factors on the relationship between adiposity and bone mass in girls. International journal of obesity (2005), 42(6), 1185-1194.
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  Childhood obesity has been separately associated with cardiometabolic risk factors (CMRs) and increased risk of fracture. However, both augmented and compromised bone mass have been reported among overweight/obese children. Metabolic dysfunction, often co-existing with obesity, may explain the discrepancy in previous studies. The aim of this study was to examine whether the relationship between adiposity and dual-energy X-ray absorptiometry (DXA) derived bone mass differed in young girls with and without CMR(s).
• Huang, S., Hu, C., Bell, M. L., Billheimer, D., Guerra, S., Roe, D., Vasquez, M. M., & Bedrick, E. J. (2018). Regularized continuous-time Markov Model via elastic net. Biometrics, 74(3), 1045-1054.
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  Continuous-time Markov models are commonly used to analyze longitudinal transitions between multiple disease states in panel data, where participants' disease states are only observed at multiple time points, and the exact state paths between observations are unknown. However, when covariate effects are incorporated and allowed to vary for different transitions, the number of potential parameters to estimate can become large even when the number of covariates is moderate, and traditional maximum likelihood estimation and subset model selection procedures can easily become unstable due to overfitting. We propose a novel regularized continuous-time Markov model with the elastic net penalty, which is capable of simultaneous variable selection and estimation for large number of parameters. We derive an efficient coordinate descent algorithm to solve the penalized optimization problem, which is fully automatic and data driven. We further consider an extension where one of the states is death, and time of death is exactly known but the state path leading to death is unknown. The proposed method is extensively evaluated in a simulation study, and demonstrated in an application to real-world data on airflow limitation state transitions.
• Kohler, L. N., Harris, R. B., Oren, E., Roe, D. J., Lance, P., & Jacobs, E. T. (2018). Adherence to Nutrition and Physical Activity Cancer Prevention Guidelines and Development of Colorectal Adenoma. Nutrients, 10(8).
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  Adherence to the American Cancer Society's (ACS) Nutrition and Physical Activity Cancer Prevention Guidelines is associated with reductions in overall cancer incidence and mortality, including site-specific cancers such as colorectal cancer. We examined the relationship between baseline adherence to the ACS guidelines and (1) baseline adenoma characteristics and (2) odds of recurrent colorectal adenomas over 3 years of follow-up. Cross-sectional and prospective analyses with a pooled sample of participants from the Wheat Bran Fiber ( = 503) and Ursodeoxycholic Acid ( = 854) trials were performed. A cumulative adherence score was constructed using baseline self-reported data regarding body size, diet, physical activity and alcohol consumption. Multivariable logistic regression demonstrated significantly reduced odds of having three or more adenomas at baseline for moderately adherent (odds ratio [OR] = 0.67, 95% confidence intervals [CI]: 0.46⁻0.99) and highly adherent (OR = 0.50, 95% CI: 0.31⁻0.81) participants compared to low adherers (-trend = 0.005). Conversely, guideline adherence was not associated with development of recurrent colorectal adenoma (moderate adherence OR = 1.16, 95% CI: 0.85⁻1.59, high adherence OR = 1.23, 95% CI: 0.85⁻1.79).
• Mandair, D., Ho, Q., Wertheim, B., Johnson, G., Roe, D., Watchman, C., & Yi, S. K. (2018). Comparison of two thermoplastic immobilization mask systems in daily volumetric image guided radiation therapy for head and neck cancers. Biomedical Physics & Engineering Express, 4(055007).
• Martinez, J. A., Yang, J., Wertheim, B. C., Roe, D. J., Schriewer, A., Lance, P., Alberts, D. S., Hammock, B. D., & Thompson, P. A. (2018). Celecoxib use and circulating oxylipins in a colon polyp prevention trial. PloS one, 13(4), e0196398.
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  Drugs that inhibit cyclooxygenase (COX)-2 and the metabolism of arachidonic acid (ARA) to prostaglandin E2 are potent anti-inflammatory agents used widely in the treatment of joint and muscle pain. Despite their benefits, daily use of these drugs has been associated with hypertension, cardiovascular and gastrointestinal toxicities. It is now recognized that ARA is metabolized to a number of bioactive oxygenated lipids (oxylipins) by cyclooxygenase (COX), lipoxygenase (LOX), and cytochrome P450 (CYP450) enzymes. Currently, the contribution of individual variability in ARA metabolism in response to the COX-2 inhibitors and potential adverse effects remains poorly understood. Using patient samples from the randomized, placebo-controlled phase III selenium/celecoxib (Sel/Cel) trial for the prevention of colorectal adenomatous polyps, we analyzed plasma concentrations of 74 oxylipins in a subset of participants who received celecoxib (n = 90) or placebo (n = 95). We assessed the effect of celecoxib (with and without low dose aspirin) on circulating oxylipins and systolic blood pressure (SBP). Individual CYP450- and LOX- but not COX-derived metabolites were higher with celecoxib than placebo (P
• Padilla-Rodriguez, M., Parker, S. S., Adams, D. G., Westerling, T., Puleo, J. I., Watson, A. W., Hill, S. M., Noon, M., Gaudin, R., Aaron, J., Tong, D., Roe, D. J., Knudsen, B., & Mouneimne, G. (2018). The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Nature communications, 9(1), 2980.
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  Estrogen promotes growth of estrogen receptor-positive (ER+) breast tumors. However, epidemiological studies examining the prognostic characteristics of breast cancer in postmenopausal women receiving hormone replacement therapy reveal a significant decrease in tumor dissemination, suggesting that estrogen has potential protective effects against cancer cell invasion. Here, we show that estrogen suppresses invasion of ER+ breast cancer cells by increasing transcription of the Ena/VASP protein, EVL, which promotes the generation of suppressive cortical actin bundles that inhibit motility dynamics, and is crucial for the ER-mediated suppression of invasion in vitro and in vivo. Interestingly, despite its benefits in suppressing tumor growth, anti-estrogenic endocrine therapy decreases EVL expression and increases local invasion in patients. Our results highlight the dichotomous effects of estrogen on tumor progression and suggest that, in contrast to its established role in promoting growth of ER+ tumors, estrogen has a significant role in suppressing invasion through actin cytoskeletal remodeling.
• Łaniewski, P., Barnes, D., Goulder, A., Cui, H., Roe, D. J., Chase, D. M., & Herbst-Kralovetz, M. M. (2018). Linking cervicovaginal immune signatures, HPV and microbiota composition in cervical carcinogenesis in non-Hispanic and Hispanic women. Scientific reports, 8(1), 7593.
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  While high-risk human papillomavirus (HPV) infection is a well-established risk factor for cervical cancer, there are likely other factors within the local microenvironment that contribute to cervical carcinogenesis. Here we investigated relationships between HPV, vaginal pH, vaginal microbiota (VMB) composition, level of genital immune mediators and severity of cervical neoplasm. We enrolled women with low- and high-grade cervical dysplasia (LGD, HGD), invasive cervical carcinoma (ICC), and healthy controls. HPV16, HPV45, HPV58, and HPV31 were the most prevalent in our cohort with HPV16 and HPV31 genotypes more prevalent in Hispanics. Vaginal pH was associated with ethnicity and severity of cervical neoplasm. Lactobacillus dominance decreased with the severity of cervical neoplasm, which correlated with elevated vaginal pH. Hispanic ethnicity was also associated with decreased Lactobacillus dominance. Furthermore, Sneathia was enriched in all precancerous groups, ICC, abnormal pH and Hispanic origin. Patients with ICC, but not LGD and HGD, exhibited increased genital inflammatory scores and elevated specific immune mediators. Notably, IL-36γ was significantly associated with ICC. Our study revealed local, host immune and microbial signatures associated with cervical carcinogenesis and provides an initial step to understanding the complex interplay between mucosal inflammation, HPV persistence and the VMB.
• Bauman, A., Ernst, K., Hayden, M., Roe, D. J., Murray, R., Agawo, M., Munga, S., Schmahl, E., & Taren, D. (2017). Assessing Community Health: An Innovative Tool for Measuring Height and Length. Journal of tropical pediatrics.
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  Anthropometric measurements, including height and length, are routinely needed for health research worldwide. Measurement boards are the current gold standard for obtaining the height and length of children. In community-based research, however, the size and weight of the measurement boards make them difficult and cumbersome to carry in the field. In addition, children and infants may express an unwillingness to be placed onto the measurement board. Electronic measuring tools commonly used in industry and contracting work are precise and portable. This study piloted a protocol to use an adapted laser measurement tool, the anthropometric measurement assist (AMA), to obtain height and recumbent length in children in Western Kenya. Intra- and inter-observer variability were determined and compared with measurement board measurements. Results of this initial pilot indicated that the AMA may be a viable alternative to measurement boards. The AMA can measure height/length accurately and reliably, is portable and is equivalent in price to measuring boards, making it a viable option for fieldwork in low-resourced countries.
• Giacobbi, P., Howe, C. L., Roe, D., Hingle, M. D., Armin, J. S., Johnson, T., Cunningham, J. K., & Gordon, J. S. (2017). Development and Evaluation of the See Me Smoke-Free Multi-Behavioral mHealth App for Women Smokers. Translational Behavioral Medicine, 7(2), 172-184.
• Gordon, J. S., Armin, J., D Hingle, M., Giacobbi, P., Cunningham, J. K., Johnson, T., Abbate, K., Howe, C. L., & Roe, D. J. (2017). Development and evaluation of the See Me Smoke-Free multi-behavioral mHealth app for women smokers. Translational behavioral medicine, 7(2), 172-184.
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  Women face particular challenges when quitting smoking, especially those with weight concerns. A multi-behavioral smoking cessation intervention addressing these concerns and incorporating guided imagery may assist women to engage in healthy lifestyle behaviors. An mHealth app can easily disseminate such an intervention. The goals of this pilot study were to develop and test the feasibility and potential of the See Me Smoke-FreemHealth app to address smoking, diet, and physical activity among women smokers. We used pragmatic, direct-to-consumer methods to develop and test program content, functionality, and the user interface and conduct a pre-/post-test, 90-day pilot study. We enrolled 151 participants. Attrition was 52%, leaving 73 participants. At 90 days, 47% of participants reported 7-day abstinence and significant increases in physical activity and fruit consumption. Recruitment methods worked well, but similar to other mHealth studies, we experienced high attrition. This study suggests that a guided imagery mHealth app has the potential to address multiple behaviors. Future research should consider different methods to improve retention and assess efficacy.
• Gordon, J. S., Cunningham, J. K., Johnson, T., Armin, J. S., Hingle, M. D., Roe, D., Howe, C. L., & Giacobbi, P. (2017). Development and Evaluation of the See Me Smoke-Free Multi-Behavioral mHealth App for Women Smokers. Translational Behavioral Medicine.
• Hassounah, N. B., Nunez, M., Fordyce, C., Roe, D., Nagle, R., Bunch, T., & McDermott, K. M. (2017). Inhibition of Ciliogenesis Promotes Hedgehog Signaling, Tumorigenesis, and Metastasis in Breast Cancer. Molecular cancer research : MCR, 15(10), 1421-1430.
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  Primary cilia are chemosensors that play a dual role to either activate or repress Hedgehog signaling, depending on presence or absence of ligand, respectively. While inhibition of ciliogenesis has been shown to be characteristic of breast cancers, the functional consequence is unknown. Here, for the first time, inhibition of ciliogenesis led to earlier tumor formation, faster tumor growth rate, higher grade tumor formation, and increased metastasis in the polyoma middle T (PyMT) mouse model of breast cancer. Inmodel systems, inhibition of ciliogenesis resulted in increased expression of Hedgehog-target genes through a mechanism involving loss of the repressor form of the GLI transcription factor (GLIR) and activation of Hedgehog target gene expression through cross-talk with TGF-alpha (TGFA) signaling. Bioinformatics analysis revealed that increased Hedgehog signaling is frequently associated with increased TGFA; signaling in patients with triple-negative breast cancers (TNBC), a particularly aggressive breast cancer subtype. These results identify a previously unrecognized role for inhibition of ciliogenesis in breast cancer progression. This study identifies inhibition of ciliogenesis as an important event for activation of Hedgehog signaling and progression of breast cancer to a more aggressive, metastatic disease.These findings change the way we understand how cancer cells turn on a critical signaling pathways and a provide rationale for developing novel therapeutic approaches to target noncanonical Hedgehog signaling for the treatment of breast cancer..
• Kim, W. S., Shin, C. N., Kathryn Larkey, L., & Roe, D. J. (2017). Development and Validation of the Narrative Quality Assessment Tool. Journal of nursing measurement, 25(1), 171-183.
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  The use of storytelling in health promotion has grown over the past 2 decades, showing promise for moving people to initiate healthy behavior change. Given the increasingly prevalent role of storytelling in health promotion research and the need to more clearly identify what storytelling elements and mediators may better predict behavior change, there is a need to develop measures to specifically assess these factors in a cultural community context. The purpose of this study is to develop and preliminarily validate a narrative quality assessment tool for measuring elements of storytelling that are predicted to affect attitude and behavior change (i.e., narrative characteristics, identification, and transportation) within a cultural community setting using a culture-centric model.
• Kohler, L. N., Hibler, E. A., Harris, R. B., Oren, E., Roe, D. J., Jurutka, P. W., & Jacobs, E. T. (2017). Greater Adherence to Cancer Prevention Guidelines Is Associated with Higher Circulating Concentrations of Vitamin D Metabolites in a Cross-Sectional Analysis of Pooled Participants from 2 Chemoprevention Trials. The Journal of nutrition.
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  Several lifestyle factors targeted by the American Cancer Society (ACS) Nutrition and Physical Activity Cancer Prevention Guidelines are also associated with circulating concentrations of vitamin D metabolites. This suggests that greater adherence to the ACS guidelines may be related to better vitamin D status.
• Lance, P., Alberts, D. S., Thompson, P. A., Fales, L., Wang, F., San Jose, J., Jacobs, E. T., Goodman, P. J., Darke, A. K., Yee, M., Minasian, L., Thompson, I. M., & Roe, D. J. (2017). Colorectal Adenomas in Participants of the SELECT Randomized Trial of Selenium and Vitamin E for Prostate Cancer Prevention. Cancer prevention research (Philadelphia, Pa.), 10(1), 45-54.
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  Selenium and vitamin E micronutrients have been advocated for the prevention of colorectal cancer. Colorectal adenoma occurrence was used as a surrogate for colorectal cancer in an ancillary study to the Selenium and Vitamin E Cancer Prevention Trial (SELECT) for prostate cancer prevention. The primary objective was to measure the effect of selenium (as selenomethionine) on colorectal adenomas occurrence, with the effect of vitamin E (as α-tocopherol) supplementation on colorectal adenoma occurrence considered as a secondary objective. Participants who underwent lower endoscopy while in SELECT were identified from a subgroup of the 35,533 men randomized in the trial. Adenoma occurrence was ascertained from the endoscopy and pathology reports for these procedures. Relative Risk (RR) estimates and 95% confidence intervals (CI) of adenoma occurrence were generated comparing those randomized to selenium versus placebo and to vitamin E versus placebo based on the full factorial design. Evaluable endoscopy information was obtained for 6,546 participants, of whom 2,286 had 1+ adenomas. Apart from 21 flexible sigmoidoscopies, all the procedures yielding adenomas were colonoscopies. Adenomas occurred in 34.2% and 35.7%, respectively, of participants whose intervention included or did not include selenium. Compared with placebo, the RR for adenoma occurrence in participants randomized to selenium was 0.96 (95% CI, 0.90-1.02; P = 0.194). Vitamin E did not affect adenoma occurrence compared with placebo (RR = 1.03; 95% CI, 0.96-1.10; P = 0.38). Neither selenium nor vitamin E supplementation can be recommended for colorectal adenoma prevention. Cancer Prev Res; 10(1); 45-54. ©2016 AACR.
• Langston, M., Dennis, L., Lynch, C., Roe, D., & Brown, H. (2017). Temporal Trends in Satellite-Derived Erythemal UVB and Implications for Ambient Sun Exposure Assessment. International journal of environmental research and public health, 14(2).
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  Ultraviolet radiation (UVR) has been associated with various health outcomes, including skin cancers, vitamin D insufficiency, and multiple sclerosis. Measurement of UVR has been difficult, traditionally relying on subject recall. We investigated trends in satellite-derived UVB from 1978 to 2014 within the continental United States (US) to inform UVR exposure assessment and determine the potential magnitude of misclassification bias created by ignoring these trends. Monthly UVB data remotely sensed from various NASA satellites were used to investigate changes over time in the United States using linear regression with a harmonic function. Linear regression models for local geographic areas were used to make inferences across the entire study area using a global field significance test. Temporal trends were investigated across all years and separately for each satellite type due to documented differences in UVB estimation. UVB increased from 1978 to 2014 in 48% of local tests. The largest UVB increase was found in Western Nevada (0.145 kJ/m2 per five-year increment), a total 30-year increase of 0.87 kJ/m2. This largest change only represented 17% of total ambient exposure for an average January and 2% of an average July in Western Nevada. The observed trends represent cumulative UVB changes of less than a month, which are not relevant when attempting to estimate human exposure. The observation of small trends should be interpreted with caution due to measurement of satellite parameter inputs (ozone and climatological factors) that may impact derived satellite UVR nearly 20% compared to ground level sources. If the observed trends hold, satellite-derived UVB data may reasonably estimate ambient UVB exposures even for outcomes with long latency phases that predate the satellite record.
• Thomson, C. A., Chow, H. H., Wertheim, B. C., Roe, D. J., Stopeck, A., Maskarinec, G., Altbach, M., Chalasani, P., Huang, C., Strom, M. B., Galons, J. P., & Thompson, P. A. (2017). A randomized, placebo-controlled trial of diindolylmethane for breast cancer biomarker modulation in patients taking tamoxifen. Breast cancer research and treatment, 165(1), 97-107.
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  Diindolylmethane (DIM), a bioactive metabolite of indole-3-carbinol found in cruciferous vegetables, has proposed cancer chemoprevention activity in the breast. There is limited evidence of clinically relevant activity of DIM or long-term safety data of its regular use. A randomized, double-blind, placebo-controlled trial was conducted to determine the activity and safety of combined use of BioResponse DIM® (BR-DIM) with tamoxifen.
• Vasquez, M. M., Hu, C., Roe, D. J., Halonen, M., & Guerra, S. (2017). Measurement error correction in the least absolute shrinkage and selection operator model when validation data are available. Statistical methods in medical research, 962280217734241.
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  Measurement of serum biomarkers by multiplex assays may be more variable as compared to single biomarker assays. Measurement error in these data may bias parameter estimates in regression analysis, which could mask true associations of serum biomarkers with an outcome. The Least Absolute Shrinkage and Selection Operator (LASSO) can be used for variable selection in these high-dimensional data. Furthermore, when the distribution of measurement error is assumed to be known or estimated with replication data, a simple measurement error correction method can be applied to the LASSO method. However, in practice the distribution of the measurement error is unknown and is expensive to estimate through replication both in monetary cost and need for greater amount of sample which is often limited in quantity. We adapt an existing bias correction approach by estimating the measurement error using validation data in which a subset of serum biomarkers are re-measured on a random subset of the study sample. We evaluate this method using simulated data and data from the Tucson Epidemiological Study of Airway Obstructive Disease (TESAOD). We show that the bias in parameter estimation is reduced and variable selection is improved.
• Chen, Y., Gao, M., Duncan, J. K., Ran, D., Roe, D. J., Belin, M. W., & Wang, M. (2016). Excisional keratectomy combined with focal cryotherapy and amniotic membrane inlay for recalcitrant filamentary fungal keratitis: A retrospective comparative clinical data analysis. Experimental and therapeutic medicine, 12(5), 3014-3020.
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  The aim of the present study was to investigate the efficacy of a novel surgical intervention, excisional keratectomy combined with focal cryotherapy and amniotic membrane inlay (EKCAI), for the treatment of recalcitrant filamentary fungal keratitis. A retrospective analysis was performed of patients who underwent excisional keratectomy combined with conjunctival flap inlay (EKCFI), EKCAI or therapeutic penetrating keratoplasty (TPK) from January 2006 to January 2011. Recalcitrance was determined as being unresponsive to standard medical antifungal therapy for at ≥1 week. Outcome measures among the three intervention modalities were compared. A total of 128 patients had a follow-up of ≥1 year after the primary intervention. The success rates of interventions at 1-year follow-up were 58.33% in the EKCFI group, 88.37% in the EKCAI group and 93.44% in the TPK group (P
• Hincapie, A., MacKinnon, N., Malone, D. C., Slack, M. K., Roe, D., & Warholak, T. L. (2016). Relationship between Patients’ Perceptions of Care Quality and Patient Safety in 11 Countries: A Secondary Data Analysis. Quality and Management in Health Care, 25(1), 13-21.
• Jacobs, E. T., Haussler, M. R., Alberts, D. S., Kohler, L. N., Lance, P., Martínez, M. E., Roe, D. J., & Jurutka, P. W. (2016). Association between Circulating Vitamin D Metabolites and Fecal Bile Acid Concentrations. Cancer prevention research (Philadelphia, Pa.), 9(7), 589-97.
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  Although hydrophobic bile acids have been demonstrated to exhibit cytotoxic and carcinogenic effects in the colorectum, ursodeoxycholic acid (UDCA) has been investigated as a potential chemopreventive agent. Vitamin D has been shown to play a role in both bile acid metabolism and in the development of colorectal neoplasia. Using a cross-sectional design, we sought to determine whether baseline circulating concentrations of the vitamin D metabolites 25(OH)D and 1,25(OH)2D were associated with baseline fecal bile acid concentrations in a trial of UDCA for the prevention of colorectal adenoma recurrence. We also prospectively evaluated whether vitamin D metabolite concentrations modified the effect of UDCA on adenoma recurrence. After adjustment for age, sex, BMI, physical activity, and calcium intake, adequate concentrations of 25(OH)D (≥30 ng/mL) were statistically significantly associated with reduced odds for high levels of total [OR, 0.61; 95% confidence interval (CI), 0.38-0.97], and primary (OR, 0.61; 95% CI, 0.38-0.96) bile acids, as well as individually with chenodeoxycholic acid (OR, 0.39; 95% CI, 0.24-0.63) and cholic acid (OR, 0.56; 95% CI, 0.36-0.90). No significant associations were observed for 1,25(OH)2D and high versus low fecal bile acid concentrations. In addition, neither 25(OH)D nor 1,25(OH)2D modified the effect of UDCA on colorectal adenoma recurrence. In conclusion, this is the first study to demonstrate an inverse relationship between circulating levels of 25(OH)D and primary fecal bile acid concentrations. These results support prior data demonstrating that vitamin D plays a key role in bile acid metabolism, and suggest a potential mechanism of action for 25(OH)D in colorectal cancer prevention. Cancer Prev Res; 9(7); 589-97. ©2016 AACR.
• Jacobs, E. T., Roe, D., Oren, E., Harris, R. B., Garcia, D. O., & Kohler, L. N. (2016). Adherence to Diet and Physical Activity Cancer Prevention Guidelines and Cancer Outcomes: A Systematic Review.. Cancer Epidemiology, Biomarkers & Prevention. doi:10.1158/1055-9965.EPI-16-0121
• Kohler, L. N., Garcia, D. O., Harris, R. B., Oren, E., Roe, D. J., & Jacobs, E. T. (2016). Adherence to Diet and Physical Activity Cancer Prevention Guidelines and Cancer Outcomes: A Systematic Review. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 25(7), 1018-28.
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  Many studies have reported that adherence to health promotion guidelines for diet, physical activity, and maintenance of healthy body weight may decrease cancer incidence and mortality. A systematic review was performed to examine associations between adherence to established cancer prevention guidelines for diet and physical activity and overall cancer incidence and mortality. PubMed, Google Scholar, and Cochrane Reviews databases were searched following the current recommendations of Preferred Reporting Items for Systematic Reviews and Meta-analysis Approach (PRISMA). Twelve studies met inclusion criteria for this review. High versus low adherence to established nutrition and physical activity cancer prevention guidelines was consistently and significantly associated with decreases of 10% to 61% in overall cancer incidence and mortality. Consistent significant reductions were also shown for breast cancer incidence (19%-60%), endometrial cancer incidence (23%-60%), and colorectal cancer incidence in both men and women (27%-52%). Findings for lung cancer incidence were equivocal, and no significant relationships were found between adherence and ovarian or prostate cancers. Adhering to cancer prevention guidelines for diet and physical activity is consistently associated with lower risks of overall cancer incidence and mortality, including for some site-specific cancers. Cancer Epidemiol Biomarkers Prev; 25(7); 1018-28. ©2016 AACR.
• Kohler, L. N., Garcia, D. O., Harris, R. B., Oren, E., Roe, D., & Jacobs, E. T. (2016). Adherence to Diet and Physical Activity Cancer Prevention Guidelines and Cancer Outcomes: A Systematic Review.. Cancer Epidemiology, Biomarkers & Prevention.
• Larkey, L. K., Roe, D. J., Smith, L., & Millstine, D. (2016). Exploratory outcome assessment of Qigong/Tai Chi Easy on breast cancer survivors. Complementary therapies in medicine, 29, 196-203.
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  Breast cancer survivors (BCSs) experience symptoms affecting overall quality of life (QOL), often for a prolonged period post-treatment. Meditative Movement (MM), including Qigong and Tai Chi Easy (QG/TCE), has demonstrated benefit for improving QOL issues such as fatigue and sleep, but there is limited evidence of its impact on cognitive function, overall physical activity, and body weight for BCSs.
• Patanwala, A. E., Erstad, B. L., Roe, D. J., & Sakles, J. C. (2016). Succinylcholine Is Associated with Increased Mortality When Used for Rapid Sequence Intubation of Severely Brain Injured Patients in the Emergency Department. Pharmacotherapy, 36(1), 57-63.
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  To compare succinylcholine and rocuronium regarding mortality in patients with traumatic brain injury (TBI) who are intubated in the emergency department (ED).
• Poplin, G. S., Roe, D. J., Burgess, J. L., Peate, W. F., & Harris, R. B. (2016). Fire fit: assessing comprehensive fitness and injury risk in the fire service. International archives of occupational and environmental health, 89(2), 251-9.
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  This study sought to develop a comprehensive measure of fitness that is predictive of injury risk and can be used in the fire service to assess individual-level health and fit-for-duty status.
• Tate, T. H., Baggett, B., Rice, P. F., Koevary, J. W., Orsinger, G. V., Nymeyer, A. C., Welge, W. A., Saboda, K., Roe, D. J., Hatch, K. D., Chambers, S. K., Utzinger, U., & Barton, J. K. (2016). Multispectral fluorescence imaging of human ovarian and fallopian tube tissue for early-stage cancer detection. Journal of biomedical optics, 21(5), 56005.
• Thompson, P. A., Ashbeck, E. L., Roe, D. J., Fales, L., Buckmeier, J., Wang, F., Bhattacharyya, A., Hsu, C. H., Chow, H. H., Ahnen, D. J., Boland, C. R., Heigh, R. I., Fay, D. E., Hamilton, S. R., Jacobs, E. T., Martinez, M. E., Alberts, D. S., & Lance, P. (2016). Selenium Supplementation for Prevention of Colorectal Adenomas and Risk of Associated Type 2 Diabetes. Journal of the National Cancer Institute, 108(12).
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  Selenium supplementation may help to prevent colorectal cancer; as precursors of colorectal cancer, colorectal adenomas are a surrogate for colorectal cancer. Selenium supplementation may increase risk of type 2 diabetes (T2D).
• Thompson, P. A., Ashbeck, E. L., Roe, D. J., Fales, L., Buckmeier, J., Wang, F., Bhattacharyya, A., Hsu, C. H., Chow, S. H., Ahnen, D. J., Boland, C. R., Heigh, R. I., Fay, D. E., Hamilton, S. R., Jacobs, E. T., Martinez, E. M., Alberts, D. S., & Lance, P. (2016). Celecoxib for the Prevention of Colorectal Adenomas: Results of a Suspended Randomized Controlled Trial. Journal of the National Cancer Institute, 108(12).
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  Cyclooxygenase (COX)-2 inhibitors such as celecoxib were designed to preserve anti-inflammatory activity without inhibiting COX-1. Downregulation of COX-2 inhibits colorectal carcinogenesis.
• Vasquez, M. M., Hu, C., Roe, D. J., Chen, Z., Halonen, M., & Guerra, S. (2016). Least absolute shrinkage and selection operator type methods for the identification of serum biomarkers of overweight and obesity: simulation and application. BMC medical research methodology, 16(1), 154.
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  The study of circulating biomarkers and their association with disease outcomes has become progressively complex due to advances in the measurement of these biomarkers through multiplex technologies. The Least Absolute Shrinkage and Selection Operator (LASSO) is a data analysis method that may be utilized for biomarker selection in these high dimensional data. However, it is unclear which LASSO-type method is preferable when considering data scenarios that may be present in serum biomarker research, such as high correlation between biomarkers, weak associations with the outcome, and sparse number of true signals. The goal of this study was to compare the LASSO to five LASSO-type methods given these scenarios.
• Baker, A. F., Malm, S. W., Pandey, R., Laughren, C., Cui, H., Roe, D., & Chambers, S. K. (2015). Evaluation of a hypoxia regulated gene panel in ovarian cancer. Cancer Microenvironment, 8(1), 45-56.
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  A panel of nine hypoxia regulated genes, selected from a previously published fifty gene panel, was investigated for its ability to predict hypoxic ovarian cancer phenotypes. All nine genes including vascular endothelial growth factor A, glucose transporter 1, phosphoglycerate mutase 1, lactate dehydrogenase A, prolyl 4-hydroxylase, alpha-polypeptide 1, adrenomedullin, N-myc downstream regulated 1, aldolase A, and carbonic anhydrase 9 were upregulated in the HEY and OVCAR-3 human ovarian cell lines cultured in vitro under hypoxic compared to normoxic conditions as measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The gene panel was also elevated in HEY xenograft tumor tissue compared to HEY cells cultured in normoxia. The HEY xenograft tissue demonstrated heterogeneous positive immunohistochemical staining for the exogenous hypoxia biomarker pimonidazole, and the hypoxia regulated protein carbonic anhydrase IX. A quantitative nuclease protection assay (qNPA) was developed which included the nine hypoxia regulated genes. The qNPA assay provided similar results to those obtained using qRT-PCR for cultured cell lines. The qNPA assay was also evaluated using paraffin embedded fixed tissues including a set of five patient matched primary and metastatic serous cancers and four normal ovaries. In this small sample set the average gene expression was higher in primary and metastatic cancer tissue compared to normal ovaries for the majority of genes investigated. This study supports further evaluation by qNPA of this gene panel as an alternative or complimentary method to existing protein biomarkers to identify ovarian cancers with a hypoxic phenotype.
• Bea, J. W., Jurutka, P. W., Hibler, E. A., Lance, P., Martinez, M. E., Roe, D. J., Molmenti, C. L., Thompson, P. A., & Jacobs, E. T. (2015). Concentrations of the Vitamin D Metabolite 1,25(OH)(2)D and Odds of Metabolic Syndrome and its Components. Metabolism: Clinical and Experimental, 64(3), 447-459.
• Bea, J. W., Thomson, C. A., Wertheim, B. C., Nicholas, J. S., Ernst, K. C., Hu, C., Jackson, R. D., Cauley, J. A., Lewis, C. E., Caan, B., Roe, D. J., & Chen, Z. (2015). Risk of Mortality According to Body Mass Index and Body Composition Among Postmenopausal Women. American Journal of Epidemiology, 182(7), 585-596.
• Chalasani, P., Marron, M., Roe, D., Clarke, K., Iannone, M., Livingston, R. B., Shan, J. S., & Stopeck, A. T. (2015). A phase I clinical trial of bavituximab and paclitaxel in patients with HER2 negative metastatic breast cancer. Cancer Medicine, 4(7), 1051-9.
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  Bavituximab is a chimeric monoclonal antibody that targets phosphatidylserine (PS). PS is externalized on cells in the tumor microenvironment when exposed to hypoxia and/or other physiological stressors. On attaching to PS, bavituximab is thought to promote antitumor immunity through its effects on PS receptors in monocytes, and myeloid-derived suppressor cells, as well as trigger antitumor effects by inducing an antibody-dependent cellular cytotoxicity on tumor-associated endothelial cells. We conducted a phase I clinical trial of bavituximab in combination with paclitaxel in patients with HER2-negative metastatic breast cancer. Patients were treated with weekly paclitaxel (80 mg/m(2) for 3/4 weeks) and weekly bavituximab (3 mg/kg for 4/4 weeks). Correlative studies included the measurement of circulating microparticles, endothelial cells, and apoptotic tumor cells by flow cytometry. Fourteen patients with metastatic breast cancer were enrolled; all were evaluable for toxicity and 13 were evaluable for response. Treatment resulted in an overall response rate (RR) of 85% with a median progression-free survival (PFS) of 7.3 months. Bone pain, fatigue, headache, and neutropenia were the most common adverse effects. Infusion-related reactions were the most common adverse event related to bavituximab therapy. Correlative studies showed an increase in the PS-expressing apoptotic circulating tumor cells in response to bavituximab, but not with paclitaxel. No changes in the number of circulating endothelial cells or apoptotic endothelial cells were observed with therapy. Platelet and monocyte-derived microparticles decreased after initiation of bavituximab. Bavituximab in combination with paclitaxel is well tolerated for treatment of patients with metastatic breast cancer with promising results observed in terms of clinical RRs and PFS. The toxicity profile of bavituximab is notable for manageable infusion-related reactions with no evidence for increased thrombogenicity. Recent preclinical data suggest that bavituximab can also promote antitumor immune activity that should be explored in future clinical trials.
• Hibler, E. A., Klimentidis, Y. C., Jurutka, P. W., Kohler, L. N., Lance, P., Roe, D. J., Thompson, P. A., & Jacobs, E. T. (2015). CYP24A1 and CYP27B1 Polymorphisms, Concentrations of Vitamin D Metabolites, and Odds of Colorectal Adenoma Recurrence. Nutrition and Cancer, 67(7), 1131-1141.
• Hingle, M. D., Turner, T., Kutob, R., Merchant, N., Roe, D. J., Stump, C., & Going, S. B. (2015). The EPIC Kids Study: a randomized family-focused YMCA-based intervention to prevent type 2 diabetes in at-risk youth. BMC Public Health, 15.
• Hingle, M. D., Turner, T., Kutob, R., Merchant, N., Roe, D. J., Stump, C., & Going, S. B. (2015). The EPIC Kids Study: a randomized family-focused YMCA-based intervention to prevent type 2 diabetes in at-risk youth. BMC public health, 15, 1253.
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  It is well established that behavioral lifestyle interventions resulting in modest weight reduction in adults can prevent or delay type 2 diabetes mellitus; however in children, successful weight management interventions are rarely found outside of controlled clinical settings. The lack of effective community-based programs is a barrier to reducing obesity prevalence and diabetes risk in children. The objective of our study is to develop and test a group-randomized family-centered community-based type 2 diabetes prevention intervention targeting at-risk children, 9- to 12-years-old.
• Hongu, N., Pope, B. T., Bilgiç, P., Orr, B. J., Suzuki, A., Kim, A. S., Merchant, N. C., & Roe, D. J. (2015). Usability of a smartphone food picture app for assisting 24-hour dietary recall: a pilot study. Nutrition Research and Practice, 9(2), 207-12.
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  The Recaller app was developed to help individuals record their food intakes. This pilot study evaluated the usability of this new food picture application (app), which operates on a smartphone with an embedded camera and Internet capability.
• Larkey, L. K., McClain, D., Roe, D. J., Hector, R. D., Lopez, A. M., Sillanpaa, B., & Gonzalez, J. (2015). Randomized Controlled Trial of Storytelling Compared to a Personal Risk Tool Intervention on Colorectal Cancer Screening in Low-Income Patients. American Journal of Health Promotion.
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  Purpose . Screening rates for colorectal cancer (CRC) lag for low-income, minority populations, contributing to poorer survival rates. A model of storytelling as culture-centric health promotion was tested for promoting CRC screening. Design . A two-group parallel randomized controlled trial. Setting . Primary care, safety-net clinics. Subjects . Low-income patients due for CRC screening, ages 50 to 75 years, speaking English or Spanish. Intervention . Patients were exposed to either a video created from personal stories composited into a drama about "Papa" receiving CRC screening, or an instrument estimating level of personal cancer risk. Patients received a health care provider referral for CRC screening and were followed up for 3 months to document adherence. Measures . Behavioral factors related to the narrative model (identification and engagement) and theory of planned behavior. Analysis . Main effects of the interventions on screening were tested, controlling for attrition factors, and demographic factor associations were assessed. Path analysis with model variables was used to test the direct effects and multiple mediator models. Results . Main effects on CRC screening (roughly half stool-based tests, half colonoscopy) did not indicate significant differences (37% and 42% screened for storytelling and risk-based messages, respectively; n = 539; 33.6% male; 62% Hispanic). Factors positively associated with CRC screening included being female, Hispanic, married or living with a partner, speaking Spanish, having a primary care provider, lower income, and no health insurance. Engagement, working through positive attitudes toward the behavior, predicted CRC screening. Conclusion . A storytelling and a personalized risk-tool intervention achieved similar levels of screening among unscreened/underscreened, low-income patients. Factors usually associated with lower rates of screening (e.g., no insurance, being Hispanic) were related to more adherence. Both interventions' engagement factor facilitated positive attitudes about CRC screening associated with behavior change.
• Larkey, L. K., Roe, D. J., Weihs, K. L., Jahnke, R., Lopez, A. M., Rogers, C. E., Oh, B., & Guillen-Rodriguez, J. (2015). Randomized Controlled Trial of Qigong/Tai Chi Easy on Cancer-Related Fatigue in Breast Cancer Survivors. Annals of Behavioral Medicine, 49(2), 165-176.
• LeGendre-McGhee, S., Rice, P. S., Wall, R. A., Sprute, K. J., Bommireddy, R., Luttman, A. M., Nagle, R. B., Abril, E. R., Farrell, K., Hsu, C., Roe, D. J., Gerner, E. W., Ignatenko, N. A., & Barton, J. K. (2015). Time-serial Assessment of Drug Combination Interventions in a Mouse Model of Colorectal Carcinogenesis Using Optical Coherence Tomography. Cancer Growth and Metastasis, 8(Suppl 1), 63-80.
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  Optical coherence tomography (OCT) is a high-resolution, nondestructive imaging modality that enables time-serial assessment of adenoma development in the mouse model of colorectal cancer. In this study, OCT was utilized to evaluate the effectiveness of interventions with the experimental antitumor agent α-difluoromethylornithine (DFMO) and a nonsteroidal anti-inflammatory drug sulindac during early [chemoprevention (CP)] and late stages [chemotherapy (CT)] of colon tumorigenesis. Biological endpoints for drug interventions included OCT-generated tumor number and tumor burden. Immunochistochemistry was used to evaluate biochemical endpoints [Ki-67, cleaved caspase-3, cyclooxygenase (COX)-2, β-catenin]. K-Ras codon 12 mutations were studied with polymerase chain reaction-based technique. We demonstrated that OCT imaging significantly correlated with histological analysis of both tumor number and tumor burden for all experimental groups (P < 0.0001), but allows more accurate and full characterization of tumor number and burden growth rate because of its time-serial, nondestructive nature. DFMO alone or in combination with sulindac suppressed both the tumor number and tumor burden growth rate in the CP setting because of DFMO-mediated decrease in cell proliferation (Ki-67, P < 0.001) and K-RAS mutations frequency (P = 0.04). In the CT setting, sulindac alone and DFMO/sulindac combination were effective in reducing tumor number, but not tumor burden growth rate. A decrease in COX-2 staining in DFMO/sulindac CT groups (COX-2, P < 0.01) confirmed the treatment effect. Use of nondestructive OCT enabled repeated, quantitative evaluation of tumor number and burden, allowing changes in these parameters to be measured during CP and as a result of CT. In conclusion, OCT is a robust minimally invasive method for monitoring colorectal cancer disease and effectiveness of therapies in mouse models.
• Stephen, R. M., Jha, A. K., Roe, D. J., Trouard, T. P., Galons, J., Kupinski, M. A., Frey, G., Cui, H., Squire, S., Pagel, M. D., Rodriguez, J. J., Gillies, R. J., & Stopeck, A. T. (2015). Diffusion MRI with Semi-Automated Segmentation Can Serve as a Restricted Predictive Biomarker of the Therapeutic Response of Liver Metastasis. Magnetic Resonance Imaging, 33(10), 1267-1273.
• Whitlow, P. G., Saboda, K., Roe, D. J., Bazzell, S., & Wilson, C. (2015). Topical Analgesia Treats Pain and Decreases Propofol Use During Lumbar Punctures in a Randomized Pediatric Leukemia Trial. Pediatric Blood & Cancer, 62(1), 85-90.
• Barr, P. M., Miller, T. P., Friedberg, J. W., Peterson, D. R., Baran, A. M., Herr, M., Spier, C. M., Cui, H., Roe, D. J., Persky, D. O., Casulo, C., Littleton, J., Schwartz, M., Puvvada, S., Landowski, T. H., Rimsza, L. M., Dorr, R. T., Fisher, R. I., Bernstein, S. H., & Briehl, M. M. (2014). Phase 2 study of imexon, a prooxidant molecule, in relapsed and refractory B-cell non-Hodgkin lymphoma. Blood, 124(8), 1259-65.
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  Lymphoma cells are subject to higher levels of oxidative stress compared with their normal counterparts and may be vulnerable to manipulations of the cellular redox balance. We therefore designed a phase 2 study of imexon (Amplimexon/NSC-714597), a prooxidant molecule, in patients with relapsed/refractory B-cell non-Hodgkin lymphoma (NHL). Imexon was administered at 1000 mg/m(2) IV daily for 5 days in 21-day cycles. Gene expression analysis performed on pretreatment tumor specimens included 13 transcripts used to generate a redox signature score, previously demonstrated to correlate with lymphoma prognosis. Twenty-two patients were enrolled having follicular (n = 9), diffuse large B-cell (DLBCL) (n = 5), mantle cell (n = 3), transformed follicular (n = 2), small lymphocytic (n = 2), and Burkitt (n = 1) lymphoma. The most common grade 3/4 adverse events were anemia (14%) and neutropenia (9%). The overall response rate was 30%, including responses in follicular lymphoma (4 of 9) and DLBCL (2 of 5). Gene expression analyses revealed CD68 and the redox-related genes, GPX1 and SOD2, as well as a higher redox score to correlate with clinical responses. Therefore, pretreatment markers of oxidative stress may identify patients likely to respond to this therapeutic approach. This trial was registered at www.clinicaltrials.gov as #NCT01314014.
• Hongu, N., Going, S. B., Orr, B. J., Hingle, M. D., Roe, D., Houtkooper, L. K., Merchant, N. C., & Yael, G. V. (2014). Mobile Technologies for Promoting Health and Physical Activity. American College of Sports Medicine, 18(4), 8-15.
• Hongu, N., Going, S. B., Orr, B. J., Merchant, N. C., Hingle, M. D., Roe, D., Greenblatt, Y. V., & Houtkooper, L. K. (2014). TACH SAVVY: Mobile Technologies for Promoting Health and Physical Activity.. ACSM's Health & Fitness Journal, 18(4), 8-15.
• Hongu, N., Pope, B. T., Bilgic, P., Orr, B. J., Suzuki, A., Kim, A. S., Merchant, N. C., & Roe, D. (2014). Usability of a smartphone food picture app for assisting 24-hour dietary recall: a pilot study.. Nutrition Research and Practice.
• Larkey, L. K., Roe, D. J., Weihs, K. L., Jahnke, R., Lopez, A. M., Rogers, C. E., Oh, B., & Guillen-Rodriguez, J. (2014). Randomized Controlled Trial of Qigong/Tai Chi Easy on Cancer-Related Fatigue in Breast Cancer Survivors. Annals of behavioral medicine : a publication of the Society of Behavioral Medicine.
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  Many breast cancer survivors experience fatigue, mood, and sleep disturbances.
• Molmenti, C. L., Hibler, E. A., Ashbeck, E. L., Thomson, C. A., Garcia, D. O., Roe, D., Harris, R. B., Lance, P., Cisneroz, M., Martinez, M. E., Thompson, P. A., & Jacobs, E. T. (2014). Sedentary behavior is associated with colorectal adenoma recurrence in men. Cancer causes & control : CCC, 25(10), 1387-95.
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  The association between physical activity and colorectal adenoma is equivocal. This study was designed to assess the relationship between physical activity and colorectal adenoma recurrence.
• Poplin, G. S., Roe, D. J., Peate, W., Harris, R. B., & Burgess, J. L. (2014). The association of aerobic fitness with injuries in the fire service. American journal of epidemiology, 179(2).
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  The aim of the present study was to understand the risk of injury in relation to fitness in a retrospective occupational cohort of firefighters in Tucson, Arizona, from 2005 to 2009. Annual medical evaluations and injury surveillance data were linked to compare levels of aerobic fitness in injured employees with those in noninjured employees. The individual outcomes evaluated included all injuries, exercise-related injuries, and sprains and strains. Time-to-event analyses were conducted to determine the association between levels of fitness and injury likelihood. Fitness, defined by relative aerobic capacity (Vo2max), was associated with injury risk. Persons in the lowest fitness level category (Vo2max 48 mL/kg/minute). Those with a Vo2max between 43 and 48 mL/kg/minute were 1.38 times (95% confidence interval: 1.06, 1.78) more likely to incur injury. Hazard ratios were found to be greater for sprains and strains. Our results suggest that improving relative aerobic capacity by 1 metabolic equivalent of task (approximately 3.5 mL/kg/minute) reduces the risk of any injury by 14%. These findings illustrate the importance of fitness in reducing the risk of injury in physically demanding occupations, such as the fire service, and support the need to provide dedicated resources for structured fitness programming and the promotion of injury prevention strategies to people in those fields.
• Smith, L. L., Larkey, L. K., Roe, D. J., Bucho-Gonzalez, J. A., Saboda, K., & Ainsworth, B. E. (2014). Self-reported physical activity patterns among low-income Latina women in Arizona. Women's health issues : official publication of the Jacobs Institute of Women's Health, 24(3), e353-61.
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  Adherence to recommendations for physical activity (PA) among adults in the United States is reported as poor, particularly among low-income Latinos. In particular, Latina women are at increased risk for inactivity and chronic illness, but national health survey reports aggregate data across demographic strata, limiting descriptive information that could guide targeted PA promotion. The purpose of the study was to determine PA patterns among a low-income group of Latina women in the southwestern United States.
• Smith, L. L., Larkey, L. K., Roe, D. R., Bunch-Gonzalez, J. A., Saboda, K., & Ainsworth, B. E. (2014). Self-reported physical activity patterns among low-income Latina women in Arizona. Womens Health Issues, 24(3), e353-361.
• Amanda F. Baker, ., Denise J. Roe, ., Cynthia Laughren, ., Janice L. Cohen, ., Heather M. Wright, ., Mary C. Clouser, ., Haiyan Cui, ., David S. Alberts, ., & Setsuko K. Chambers, . (2013). Investigation of bendamustine HCL in a Phase 2 study in women with resistant ovarian cancer. Investigational New Drugs, 31(1), 160-166.
• Angela N. Bartley, ., Nila Parikh, ., Chiu-Hsieh Hsu, ., Denise J. Roe, ., Julie A. Buckmeier, ., Lynda Corley, ., Ron A. Phipps, ., Gary Gallick, ., Peter Lance, ., Patricia A. Thompson, ., & Stanley R. Hamilton, . (2013). Colorectal adenoma stem-like cell populations: Associations with adenoma characteristics and metachronous colorectal neoplasia. Cancer Prevention Research, 6(11), 1162-1170.
• Bartley, A. N., Parikh, N., Hsu, C., Roe, D. J., Buckmeier, J. A., Corley, L., Phipps, R. A., Gallick, G., Lance, P., Thompson, P. A., & Hamilton, S. R. (2013). Colorectal adenoma stem-like cell populations: associations with adenoma characteristics and metachronous colorectal neoplasia. Cancer prevention research (Philadelphia, Pa.), 6(11).
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  Cancer stem cells have tumor-initiation and tumor-maintenance capabilities. Stem-like cells are present in colorectal adenomas, but their relationship to adenoma pathology and patient characteristics, including metachronous development of an additional adenoma ("recurrence"), has not been studied extensively. We evaluated the expression of aldehyde dehydrogenase isoform 1A1 (ALDH1A1), a putative stem cell marker, in baseline adenomas from the placebo arm of chemoprevention trial participants with colonoscopic follow-up. An exploratory set of 20 baseline adenomas was analyzed by ALDH1A1 immunohistochemistry with morphometry, and a replication set of 89 adenomas from 76 high-risk participants was evaluated by computerized image analysis. ALDH1A1-labeling indices (ALI) were similar across patient characteristics and in advanced and nonadvanced adenomas. There was a trend toward higher ALIs in adenomas occurring in the right than left colon (P = 0.09). ALIs of synchronous adenomas were correlated (intraclass correlation coefficient 0.67). Participants in both sample sets who developed a metachronous adenoma had significantly higher ALIs in their baseline adenoma than participants who remained adenoma free. In the replication set, the adjusted odds for metachronous adenoma increased 1.46 for each 10% increase in ALIs (P = 0.03). A best-fit algorithm-based cutoff point of 22.4% had specificity of 75.0% and positive predictive value of 70.0% for metachronous adenoma development. A larger population of ALDH1A1-expressing cells in an adenoma is associated with a higher risk for metachronous adenoma, independent of adenoma size or histopathology. If confirmed, ALDH1A1 has potential as a novel biomarker in risk assessment and as a potential stem cell target for chemoprevention.
• Christine L. Sardo, ., Joseph P. Kitzmiller, ., Glen Apseloff, ., Robin B. Harris, ., Denise J. Roe, ., Gary D. Stoner, ., & Elizabeth T. Jacobs, . (2013). An open-label randomized crossover trial of lyophilized black raspberries on postprandial inflammation in older overweight males: A pilot study. American Journal of Therapeutics.
• Hongu, N., Orr, B. J., Roe, D. J., Reed, R. G., & Going, S. B. (2013). Global positioning system watches for estimating energy expenditure. Journal of strength and conditioning research / National Strength & Conditioning Association, 27(11).
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  Global positioning system (GPS) watches have been introduced commercially, converting frequent measurements of time, location, speed (pace), and elevation into energy expenditure (EE) estimates. The purpose of this study was to compare EE estimates of 4 different GPS watches (Forerunner, Suunto, Polar, Adeo), at various walking speeds, with EE estimate from a triaxial accelerometer (RT3), which was used as a reference measure in this study. Sixteen healthy young adults completed the study. Participants wore 4 different GPS watches and an RT3 accelerometer and walked at 6-minute intervals on an outdoor track at 3 speeds (3, 5, and 7 km/hr). The statistical significance of differences in EE between the 3 watches was assessed using linear contrasts of the coefficients from the overall model. Reliability across trials for a given device was assessed using intraclass correlation coefficients as estimated in the mixed model. The GPS watches demonstrated lower reliability (intraclass correlation coefficient) across trials when compared with the RT3, particularly at the higher speed, 7 km/hr. Three GPS watches (Forerunner, Polar, and Suunto) significantly and consistently underestimated EE compared with the reference EE given by the RT3 accelerometer (average mean difference: Garmin, -50.5%; Polar, -41.7%; and Suunto, -41.7%; all p < 0.001). Results suggested that caution should be exercised when using commercial GPS watches to estimate EE in athletes during field-based testing and training.
• Nadia B. Hassounah, ., Ray Nagle, ., Kathylynn Saboda, ., Denise J. Roe, ., Bruce L. Dalkin, ., & Kimberly M. McDermott, . (2013). Primary Cilia Are Lost in Preinvasive and Invasive Prostate Cancer. PLoS ONE, 8(7).
• Nelson-Moseke, A. C., Jeter, J. M., Cui, H., Roe, D. J., Chambers, S. K., & Laukaitis, C. M. (2013). An unusual BRCA mutation distribution in a high risk cancer genetics clinic. Familial cancer, 12(1).
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  The Database of Individuals at High Risk for Breast, Ovarian, or Other Hereditary Cancers at the Arizona Cancer Center in Tucson, Arizona assesses cancer risk factors and outcomes in patients with a family history of cancer or a known genetic mutation. We analyzed the subset of clinic probands who carry deleterious BRCA gene mutations to identify factors that could explain why mutations in BRCA2 outnumber those in BRCA1. Medical, family, social, ethnic and genetic mutation histories were collected from consenting patients' electronic medical records. Differences between BRCA1 and BRCA2 probands from this database were analyzed for statistical significance and compared to published analyses. A significantly higher proportion of our clinic probands carry mutations in BRCA2 than BRCA1, compared with previous reports of mutation prevalence. This also holds true for the Hispanic sub-group. Probands with BRCA2 mutations were significantly more likely than their BRCA1 counterparts to present to the high risk clinic without a diagnosis of cancer. Other differences between the groups were not significant. Six previously unreported BRCA2 mutations appear in our clinic population. The increased proportion of probands carrying deleterious BRCA2 mutations is likely multifactorial, but may reflect aspects of Southern Arizona's unique ethnic heritage.
• Nobuko Hongu, ., Barron J. Orr, ., Denise J. Roe, ., Rebecca G. Reed, ., & Scott B. Going, . (2013). Global positioning system watches for estimating energy expenditure. Journal of Strength and Conditioning Research, 27(11), 3216-3220.
• Phipps-Yonas, H., Cui, H., Sebastiao, N., Brunhoeber, P. S., Haddock, E., Deymier, M. J., Klapper, W., Lybarger, L., Roe, D. J., & Hastings, K. T. (2013). Low GILT Expression is Associated with Poor Patient Survival in Diffuse Large B-Cell Lymphoma. Frontiers in immunology, 4.
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  The major histocompatibility complex (MHC) class II-restricted antigen processing pathway presents antigenic peptides acquired in the endocytic route for the activation of CD4(+) T cells. Multiple cancers express MHC class II, which may influence the anti-tumor immune response and patient outcome. Low MHC class II expression is associated with poor survival in diffuse large B-cell lymphoma (DLBCL), the most common form of aggressive non-Hodgkin lymphoma. Therefore, we investigated whether gamma-interferon-inducible lysosomal thiol reductase (GILT), an upstream component of the MHC class II-restricted antigen processing pathway that is not regulated by the transcription factor class II transactivator, may be important in DLBCL biology. GILT reduces protein disulfide bonds in the endocytic compartment, exposing additional epitopes for binding to MHC class II and facilitating antigen presentation. In each of four independent gene expression profiling cohorts with a total of 585 DLBCL patients, low GILT expression was significantly associated with poor overall survival. In contrast, low expression of a classical MHC class II gene, HLA-DRA, was associated with poor survival in one of four cohorts. The association of low GILT expression with poor survival was independent of established clinical and molecular prognostic factors, the International Prognostic Index and the cell of origin classification, respectively. Immunohistochemical analysis of GILT expression in 96 DLBCL cases demonstrated variation in GILT protein expression within tumor cells which correlated strongly with GILT mRNA expression. These studies identify a novel association between GILT expression and clinical outcome in lymphoma. Our findings underscore the role of antigen processing in DLBCL and suggest that molecules targeting this pathway warrant investigation as potential therapeutics.
• Christina A. Cutshaw, ., Lisa K. Staten, ., Kerstin M. Reinschmidt, ., Christopher Davidson, ., & Denise J. Roe, . (2012). Depressive symptoms and health-related quality of life among participants in the pasos adelante chronic disease prevention and control program, Arizona, 2005-2008. Preventing Chronic Disease, 9(1).
• Linda K. Larkey, ., Patricia M. Herman, ., Denise J. Roe, ., Francisco Garcia, ., A.M. Lopez, ., J. Gonzalez, ., Prasadini N. Perera, ., & Kathylynn Saboda, . (2012). A cancer screening intervention for underserved Latina women by Lay Educators. Journal of Women's Health, 21(5), 557-566.
• Lisa K. Staten, ., Christina A. Cutshaw, ., Christopher Davidson, ., Kerstin Reinschmidt, ., Rosie Stewart, ., & Denise J. Roe, . (2012). Effectiveness of the pasos adelante chronic disease prevention and control program in a US-Mexico border community, 2005-2008. Preventing Chronic Disease, 9(1).
• Paloma I. Beamer, ., Christina A. Elish, ., Denise J. Roe, ., Miranda M. Loh, ., & David W. Layton, . (2012). Differences in metal concentration by particle size in house dust and soil. Journal of Environmental Monitoring, 14(3), 839-844.
• Patricia Thompson, ., Denise J. Roe, ., Liane Fales, ., Julie Buckmeier, ., Fang Wang, ., Stanley R. Hamilton, ., Achyut Bhattacharyya, ., Sylvan Green, ., Chiu-Hsieh Hsu, ., H.-H. Sherry Chow, ., Dennis J. Ahnen, ., C. Richard Boland, ., Russell I. Heigh, ., David E. Fay, ., Maria Elena Martinez, ., Elizabeth Jacobs, ., Erin L. Ashbeck, ., David S. Alberts, ., & Peter Lance, . (2012). Design and baseline characteristics of participants in a phase III randomized trial of celecoxib and selenium for colorectal adenoma prevention. Cancer Prevention Research, 5(12), 1381-1393.
• Roe, D., Cutshaw, C. A., Staten, L. K., Reinschmidt, K. M., Davidson, C., & Roe, D. -. (2012). Depressive symptoms and health-related quality of life among participants in the Pasos Adelante chronic disease prevention and control program, Arizona, 2005-2008. Preventing chronic disease, 9.
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  Chronic diseases are the leading causes of death in the United States and have been associated with depressive symptoms and poor health-related quality of life (HRQOL). This study examined whether depressive symptoms and HRQOL indicators changed among participants in Pasos Adelante, a chronic disease prevention and control program implemented in a US-Mexico border community.
• Liang-Chih Liu, ., Julie E. Lang, ., Ying Lu, ., Denise Roe, ., Shelley E. Hwang, ., Cheryl A. Ewing, ., Laura J. Esserman, ., Eugene Morita, ., Patrick Treseler, ., & Stanley P. Leong, . (2011). Intraoperative frozen section analysis of sentinel lymph nodes in breast cancer patients. Cancer, 117(2), 250-258.
• Ursa Broum-Glaberman, ., Michelle Cordoba Kissee, ., Mary Lou Damiano, ., Denise J. Roe, ., & Alison T. Stopeck, . (2011). Decreased bone mineral density in adult patients with Hemophilia: Clinical significance, prevalence and risk factors. Journal of Coagulation Disorders, 3(1), 27-33.
• Wenqing Qi, ., Laurence S. Cooke, ., Xiaobing Liu, ., Lisa Rimsza, ., Denise J. Roe, ., Ann Manziolli. Daniel O. Persky, ., Thomas P. Miller, ., & Daruka Mahadevan, . (2011). Aurora inhibitor MLN8237 in combination with docetaxel enhances apoptosis and anti-tumor activity in mantle cell lymphoma. Biochemical Pharmacology, 81(7), 881-890.
• Mary C. Clouser, ., Denise J. Roe, ., Janet A. Foote, ., Robin B. Harris, ., & David S. Alberts, . (2010). Dose response of retinol and isotretinoin in the prevention of nonmelanoma skin cancer recurrence. Nutrition and Cancer, 62(8), 1058-1066.
• Setsuko K. Chambers, ., Mary C. Clouser, ., Amanda F. Baker, ., Denise J. Roe, ., Haiyan Cui, ., Molly A. Brewer, ., Kenneth D. Hatch, ., Michael S. Gordon, ., Mike F. Janicek, ., Jeffrey D. Isaacs, ., Alan N. Gordon, ., Raymond B. Nagle, ., Heather M. Wright, ., Janice L. Cohen, ., & David S. Alberts, . (2010). Overexpression of tumor vascular endothelial growth factor A may portend an increased likelihood of progression in a phase II trial of bevacizumab and erlotinib in resistant ovarian cancer. Clinical Cancer Research, 16(21), 5320-5328.
• Betsy C. Wertheim, ., María Elena Martínez, ., Erin L. Ashbeck, ., Denise J. Roe, ., Elizabeth T. Jacobs, ., David S. Alberts, ., & Patricia A. Thompson, . (2009). Physical activity as a determinant of fecal bile acid levels. Cancer Epidemiology Biomarkers and Prevention, 18(5), 1591-1598.
• Ian F. Robey, ., Brenda K. Baggett, ., Nathaniel D. Kirkpatrick, ., Denise J. Roe, ., Julie Dosescu, ., Bonnie F. Sloane, ., Arig Ibrahim Hashini, ., David L. Morse, ., Natarajan Raghunand, ., Robert A. Gatenby, ., & Robert J. Gillies, . (2009). Bicarbonate increases tumor pH and inhibits spontaneous metastases. Cancer Research, 69(6), 2260-2268.
• Janet A. Foote, ., James R. Ranger-Moore, ., Janine G. Einspahr, ., Kathylynn Saboda, ., Jaime Kenyon, ., James Warneke, ., Richard C. Miller, ., Rayna Goldman, ., Min-Jian Xu, ., Denise J. Roe, ., & David S. Alberts, . (2009). Chemoprevention of human actinic keratoses by topical DL-α-tocopherol. Cancer Prevention Research, 2(4), 394-400.
• M.A. Chisholm-Burns, ., C.A. Spivey, ., R. Rehfeld, ., M. Zawaideh, ., D.J. Roe, ., & R. Gruessner, . (2009). Immunosuppressant therapy adherence and graft failure among pediatric renal transplant recipients. American Journal of Transplantation, 9(11), 2497-2504.
• Mary C. Clouser, ., Denise J. Roe, ., Janet A. Foote, ., & Robin B. Harris, . (2009). Effect of non-steroidal anti-inflammatory drugs on non-melanoma skin cancer incidence in the SKICAP-AK trial. Pharmacoepidemiology and Drug Safety, 18(4), 276-283.
• Patricia A. Thompson, ., Betsy C. Wertheim, ., Denise J. Roe, ., Erin L. Ashbeck, ., Elizabeth T. Jacobs, ., Peter Lance, ., María Elena Martínez, ., & David S. Alberts, . (2009). Gender modifies the effect of ursodeoxycholic acid in a randomized controlled trial in colorectal adenoma patients. Cancer Prevention Research, 2(12), 1023-1030.
• Claire C. Venker, ., James L. Goodwin, ., Denise J. Roe, ., Kristine L. Kaemingk, ., Shelagh Mulvaney, ., & Stuart F. Quan, . (2007). Normative psychomotor vigilance task performance in children ages 6 to 11 - The Tucson Children's Assessment of Sleep Apnea (TuCASA). Sleep and Breathing, 11(4), 217-224.
• Amanda F. Baker, ., Tomislav Dragovich, ., Wendy R. Tate, ., Ramesh K. Ramanathan, ., Denise Roe, ., Chiu-Hsieh Hsu, ., D. Lynn Kirkpatrick, ., & Garth Powis, . (2006). The antitumor thioredoxin-1 inhibitor PX-12 (1-methylpropyl 2-imidazolyl disulfide) decreases thioredoxin-1 and VEGF levels in cancer patient plasma. Journal of Laboratory and Clinical Medicine, 147(2), 83-90.
• Frank G. Walter, ., Peter B. Chase, ., Miguel C. Fernandez, ., Diane Cameron, ., Denise J. Roe, ., & Mark Wolfson, . (2006). Pyridoxine does not prevent hyperbaric oxygen-induced seizures in rats. Journal of Emergency Medicine, 31(2), 135-138.
• Mary C. Clouser, ., Robin B. Harris, ., Denise J. Roe, ., Kathylynn Saboda, ., James Ranger-Moore, ., Laura Duckett, ., & David S. Alberts, . (2006). Risk group, skin lesion history, and sun sensitivity reliability in squamous cell skin cancer progression. Cancer Epidemiology Biomarkers and Prevention, 15(11), 2292-2297.
• Alan List, ., Sandy Kurtin, ., Denise J. Roe, ., Andrew Buresh, ., Daruka Mahadevan, ., Deborah Fuchs, ., Lisa Rimsza, ., Ruth Heaton, ., Robert Knight, ., & Jerome B. Zeldis, . (2005). Efficacy of lenalidomide in myelodysplastic syndromes. New England Journal of Medicine, 352(6), 549-557.
• David S. Alberts, ., María Elena Martínez, ., Lisa M. Hess, ., Janine G. Einspahr, ., Sylvan B. Green, ., A.K. Bhattacharyya, ., Jose Guillen, ., Mary Krutzsch, ., Ashok K. Batta, ., Gerald Salen, ., Liane Fales, ., Kris Koonce, ., Dianne Parish, ., Mary Clouser, ., Denise Roe, ., & Peter Lance, . (2005). Phase III trial of ursodeoxycholic acid to prevent colorectal adenoma recurrence. Journal of the National Cancer Institute, 97(11), 846-853.
• Denise H. Frank, ., Denise J. Roe, ., H.-H. Sherry Chow, ., Jose M. Guillen, ., Karin Choquette, ., Debra Gracie, ., Jennifer Francis, ., Airley Fish, ., & David S. Alberts, . (2004). Effects of a High-Selenium Yeast Supplement on Celecoxib Plasma Levels: A Randomized Phase II Trial. Cancer Epidemiology Biomarkers and Prevention, 13(2), 299-303.
• Elizabeth T. Jacobs, ., Anna R. Giuliano, ., Denise J. Roe, ., José M. Guillén-Rodríguez, ., Vernon L. Hartz, ., Robin C. Whitacre, ., David S. Alberts, ., & María Elena Martínez, . (2004). Dietary change in an intervention trial of wheat bran fiber and colorectal adenoma recurrence. Annals of Epidemiology, 14(4), 280-286.
• Erin M. Siegel, ., Neal E. Craft, ., Denise J. Roe, ., Eliane Duarte-Franco, ., Luisa L. Villa, ., Eduardo L. Franco, ., & Anna R. Giuliano, . (2004). Temporal variation and identification of factors associated with endogenous retinoic acid isomers in serum from brazilian women. Cancer Epidemiology Biomarkers and Prevention, 13(11), 1693-1703.
• Zhao Chen, ., William A. Stini, ., James R. Marshall, ., Maria Elena Martínez, ., Jose. M. Guillén-Rodríguez, ., Denise Roe, ., & David S. Alberts, . (2004). Wheat bran fiber supplementation and bone loss among older people. Nutrition, 20(9), 747-751.
• Anna R. Giuliano, ., Erin M. Siegel, ., Denise J. Roe, ., Silvandeiede Ferreira, ., Maria Luiza Baggio, ., Lenice Galan, ., Eliane Duarte-Franco, ., Luisa L. Villa, ., Thomas E. Rohan, ., James R. Marshall, ., & Eduardo L. Franco, . (2003). Dietary Intake and Risk of Persistent Human Papillomavirus (HPV) Infection: The Ludwig-McGill HPV Natural History Study. Journal of Infectious Diseases, 188(10), 1508-1516.
• David S. Alberts, ., Janine G. Einspahr, ., David L. Earnest, ., Mary F. Krutzsch, ., Po Lin, ., Lisa M. Hess, ., David K. Heddens, ., Denise J. Roe, ., Maria Elena Martínez, ., Gerald Salen, ., & A.K. Batta, . (2003). Fecal bile acid concentrations in a subpopulation of the wheat bran fiber colon polyp trial. Cancer Epidemiology Biomarkers and Prevention, 12(3), 197-200.
• Graham Nichol, ., T. Valenzuela, ., D. Roe, ., L. Clark, ., E. Huszti, ., & G.A. Wells, . (2003). Cost effectiveness of defibrillation by targeted responders in public settings. Circulation, 108(6), 697-703.
• Mary E. Reid, ., James R. Marshall, ., Denise Roe, ., Michael Lebowitz, ., David Alberts, ., Achyut K. Battacharyya, ., & Maria Elena Martinez, . (2003). Smoking Exposure as a Risk Factor for Prevalent and Recurrent Colorectal Adenomas. Cancer Epidemiology Biomarkers and Prevention, 12(10), 1006-1011.
• Anna R. Giuliano, ., Rebecca L. Sedjo, ., Denise J. Roe, ., Robin Harris, ., Susie Baldwin, ., Mary R. Papenfuss, ., Martha Abrahamsen, ., & Paula Inserra, . (2002). Clearance of oncogenic human papillomavirus (HPV) infection: Effect of smoking (United States). Cancer Causes and Control, 13(9), 839-846.
• Anna R. Giuliano, ., Robin Harris, ., Rebecca L. Sedjo, ., Susie Baldwin, ., Denise Roe, ., Mary R. Papenfuss, ., Martha Abrahamsen, ., Paula Inserra, ., Sandra Olvera, ., & Kenneth Hatch, . (2002). Incidence, prevalence, and clearance of type-specific human papillomavirus infections: The Young Women's Health Study. Journal of Infectious Diseases, 186(4), 462-469.
• David Heddens, ., David S. Alberts, ., Edward V. Hannigan, ., Steven D. Williams, ., Dava Garcia, ., Denise J. Roe, ., Jeffrey Bell, ., & Ronald D. Alvarez, . (2002). Prediction of the need for red cell transfusion in newly diagnosed ovarian cancer patients undergoing platinum-based treatment. Gynecologic Oncology, 86(3), 239-243.
• Elizabeth T. Jacobs, ., Anna R. Giuliano, ., Denise J. Roe, ., José M. Guillén-Rodríguez, ., David S. Alberts, ., & María Elena Martínez, . (2002). Baseline dietary fiber intake and colorectal adenoma recurrence in the wheat bran fiber randomized trial. Journal of the National Cancer Institute, 94(21), 1620-1625.
• Elizabeth T. Jacobs, ., Anna R. Giuliano, ., Denise J. Roe, ., José M. Guillén-Rodríguez, ., Lisa M. Hess, ., David S. Alberts, ., & María Elena Martínez, . (2002). Intake of supplemental and total fiber and risk of colorectal adenoma recurrence in the wheat bran fiber trial. Cancer Epidemiology Biomarkers and Prevention, 11(9), 906-914.
• Harris Bernstein, ., Hana Holubec, ., James A. Warneke, ., Harinder Garewal, ., David L. Earnest, ., Claire M. Payne, ., Denise J. Roe, ., Haiyan Cui, ., Elaine L. Jacobson, ., & Carol Bernstein, . (2002). Patchy field defects of apoptosis resistance and dedifferentiation in flat mucosa of colon resections from colon cancer patients. Annals of Surgical Oncology, 9(5), 505-517.
• Rebecca L. Sedjo, ., Denise J. Roe, ., Martha Abrahamsen, ., Robin B. Harris, ., Neal Craft, ., Susie Baldwin, ., & Anna R. Giuliano, . (2002). Vitamin A, carotenoids, and risk of persistent oncogenic human papillomavirus infection. Cancer Epidemiology Biomarkers and Prevention, 11(9), 876-884.
• Rebecca L. Sedjo, ., Paula Inserra, ., Martha Abrahamsen, ., Robin B. Harris, ., Denise J. Roe, ., Susie Baldwin, ., & Anna R. Giuliano, . (2002). Human papillomavirus persistence and nutrients involved in the methylation pathway among a cohort of young women. Cancer Epidemiology Biomarkers and Prevention, 11(4), 353-359.
• Janet A. Foote, ., Robin B. Harris, ., Anna R. Giuliano, ., Denise J. Roe, ., Thomas E. Moon, ., Brenda Cartmel, ., & David S. Alberts, . (2001). Predictors for cutaneous basal- and squamous-cell carcinoma among actinically damaged adults. International Journal of Cancer, 95(1), 7-11.
• R. Dorr, ., C. Karanes, ., C. Spier, ., T. Grogan, ., J. Greer, ., J. Moore, ., B. Weinberger, ., G. Schiller, ., T. Pearce, ., M. Litchman, ., W. Dalton, ., D. Roe, ., & A.F. List, . (2001). Phase I/II study of the P-glycoprotein modulator PSC 833 in patients with acute myeloid leukemia. Journal of Clinical Oncology, 19(6), 1589-1599.
• Anabel L. Hill, ., Denise J. Roe, ., Douglas L. Taren, ., Myra M. Muramoto, ., & Scott J. Leischow, . (2000). Efficacy of transdermal nicotine in reducing post-cessation weight gain in a Hispanic sample. Nicotine and Tobacco Research, 2(3), 247-253.
• Antonio T. Baines, ., Hanna Holubec, ., Jenny L. Basye, ., Patricia Thorne, ., Achyut K. Bhattacharyya, ., Julian Spallholz, ., Brent Shriver, ., Haiyan Cui, ., Denise Roe, ., Larry C. Clark, ., David L. Earnest, ., & Mark A Nelson, . (2000). The effects of dietary selenomethionine on polyamines and azoxymethane-induced aberrant crypts. Cancer Letters, 160(2), 193-198.
• C.L. Crowley, ., C.M. Payne, ., H. Bernstein, ., C. Bernstein, ., & D. Roe, . (2000). The NAD + precursors, nicotinic acid and nicotinamide protect cells against apoptosis induced by a multiple stress inducer, deoxycholate. Cell Death and Differentiation, 7(3), 314-326.
• D.S. Alberts, ., R.T. Dorr, ., J.G. Einspahr, ., M. Aickin, ., K. Saboda, ., M.J. Xu, ., Y.-M. Peng, ., R. Goldman, ., J.A. Foote, ., J.A. Warneke, ., S. Salasche, ., D.J. Roe, ., & G.T. Bowden, . (2000). Chemoprevention of human actinic keratoses by topical 2-(Difluoromethyl)-dl-ornithine. Cancer Epidemiology Biomarkers and Prevention, 9(12), 1281-1286.
• David S. Alberts, ., María Elena Martínez, ., Denise J. Roe, ., José M. Guillén-Rodríguez, ., James R. Marshall, ., J. Barbara Van Leeuwen, ., Mary E. Reid, ., Cheryl Ritenbaugh, ., Perla A. Vargas, ., A.B. Bhattacharyya, ., David L. Earnest, ., Richard E. Sampliner, ., Dianne Parish, ., Kris Koonce, ., & Lianne Fales, . (2000). Erratum: Lack of effect of a high-fiber cereal supplement on the recurrence of colorectal adenomas (New England Journal of Medicine (April 20, 2000) 342 (1156-1162)). New England Journal of Medicine, 343(13), 980.
• David S. Alberts, ., María Elena Martínez, ., Denise J. Roe, ., José M. Guillén-Rodríguez, ., James R. Marshall, ., J. Barbara Van Leeuwen, ., Mary E. Reid, ., Cheryl Ritenbaugh, ., Perla A. Vargas, ., A.B. Bhattacharyya, ., David L. Earnest, ., Richard E. Sampliner, ., Dianne Parish, ., Kris Koonce, ., & Lianne Fales, . (2000). Lack of effect of a high-fiber cereal supplement on the recurrence of colorectal adenomas. New England Journal of Medicine, 342(16), 1156-1162.
• H.-H. Sherry Chow, ., David L. Earnest, ., David Clark, ., Nancy Mason-Liddil, ., Cheryl B. Kramer, ., Janine G. Einspahr, ., Jose M. Guillen-Rodriguez, ., Denise J. Roe, ., Winfred Malone, ., James A. Crowell, ., & David S. Alberts, . (2000). Effect of subacute ibuprofen dosing on rectal mucosal prostaglandin E 2 levels in healthy subjects with a history of resected polyps. Cancer Epidemiology Biomarkers and Prevention, 9(4), 351-356.
• Mark A Nelson, ., Michael D Radmacher, ., Richard Simon, ., Mikel Aickin, ., Jin-Ming Yang, ., Lita Panda, ., Julia Emerson, ., Denise Roe, ., Lawrence Adair, ., Floyd Thompson, ., Jerry Bangert, ., Stanley P.L Leong, ., Raymond Taetle, ., Sydney Salmon, ., & Jeffrey Trent, . (2000). Chromosome abnormalities in malignant melanoma: Clinical significance of nonrandom chromosome abnormalities in 206 cases. Cancer Genetics and Cytogenetics, 122(2), 101-109.
• R. Calaluce, ., D.L. Earnest, ., D. Heddens, ., J.G. Einspahr, ., D. Roe, ., C.L. Bogert, ., J.R. Marshall, ., & D.S. Alberts, . (2000). Effects of piroxicam on prostaglandin E 2 levels in rectal mucosa of adenomatous polyp patients: A randomized phase IIb trial 1. Cancer Epidemiology Biomarkers and Prevention, 9(12), 1287-1292.
• Terence D. Valenzuela, ., Denise J. Roe, ., Graham Nichol, ., Lani L. Clark, ., Daniel W. Spaite, ., & Richard G. Hardman, . (2000). Outcomes of rapid defibrillation by security officers after cardiac arrest in casinos. New England Journal of Medicine, 343(17), 1206-1209.
• B. Risendal, ., D. Roe, ., J. DeZapien, ., M. Papenfuss, ., & A. Giuliano, . (1999). Influence of health care, cost, and culture on breast cancer screening: Issues facing urban American Indian women. Preventive Medicine, 29(6 I), 501-509.
• Carol Bernstein, ., Harris Bernstein, ., Harinder Garewal, ., Pat Dinning, ., Rawah Jabi, ., Richard E. Sampliner, ., Margaret K. McCuskey, ., Madhulita Panda, ., Denise J Roe, ., Lawrence L'Heureux, ., & Claire Payne, . (1999). A bile acid-induced apoptosis assay for colon cancer risk and associated quality control studies. Cancer Research, 59(10), 2353-2357.
• David L. Earnest, ., Richard E. Sampliner, ., Denise J. Roe, ., Barbara Van Leeuwen, ., Jose Guillen, ., Mary Reid, ., Maria Elena Martinez, ., James R. Marshall, ., & David S. Alberts, . (1999). Progress report: The Arizona phase III study of the effect of wheat bran fiber on recurrence of adenomatous colon polyps. American Journal of Medicine, 106(1 A), 43S-45S.
• María Elena Martínez, ., David Heddens, ., David L. Earnest, ., Cheryl L. Bogert, ., Denise Roe, ., Janine Einspahr, ., James R. Marshall, ., & David S. Alberts, . (1999). Physical activity, body mass index, and prostaglandin E 2 levels in rectal mucosa. Journal of the National Cancer Institute, 91(11), 950-953.
• N. Raghunand, ., X. He, ., R. Van Sluis, ., B. Mahoney, ., B. Baggett, ., C.W. Taylor, ., G. Paine-Murrieta, ., D. Roe, ., Z.M. Bhujwalla, ., & R.J. Gillies, . (1999). Enhancement of chemotherapy by manipulation of tumour pH. British Journal of Cancer, 80(7), 1005-1011.
• Raymond Taetle, ., Mikel Aickin, ., Jin-Ming Yang, ., Lita Panda, ., Julia Emerson, ., Denise Roe, ., Lawrence Adair, ., Floyd Thompson, ., Yun Liu, ., Lee Wisner, ., John R. Davis, ., Jeffrey Trent, ., & David S. Alberts, . (1999). Chromosome abnormalities in ovarian adenocarcinoma: I. Nonrandom chromosome abnormalities from 244 cases. Genes Chromosomes and Cancer, 25(3), 290-300.
• Raymond Taetle, ., Mikel Aickin, ., Lita Panda, ., Julia Emerson, ., Roe Denise, ., Floyd Thompson, ., John Davis, ., Jeffrey Trent, ., & David Alberts, . (1999). Chromosome abnormalities in ovarian adenocarcinoma: II. Prognostic impact of nonrandom chromosome abnormalities in 244 cases. Genes Chromosomes and Cancer, 25(1), 46-52.
• A. Hill, ., D. Taren, ., D. Roe, ., T. Lohman, ., & S. Leischow, . (1998). Body composition changes in hispanics after smoking cessation. FASEB Journal, 12(5), A862.
• Claire Redman, ., Julie A. Scott, ., Antonio T. Baines, ., Jenny L. Basye, ., Larry C. Clark, ., Cindy Calley, ., Denise Roe, ., Claire M. Payne, ., & Mark A. Nelson, . (1998). Inhibitory effect of selenomethionine on the growth of three selected human tumor cell lines. Cancer Letters, 125(1-2), 103-110.
• F.G. Walter, ., R.L. Gibly, ., R.K. Knopp, ., & D.J. Roe, . (1998). Squamous cells as predictors of bacterial contamination in urine samples. Annals of Emergency Medicine, 31(4), 455-458.
• Yei-Mei Peng, ., Yeh-Shan Peng, ., Joel M. Childers, ., Kenneth D. Hatch, ., Denise J. Roe, ., Yonggu Lin, ., & Po Lin, . (1998). Concentrations of carotenoids, tocopherols, and retinol in paired plasma and cervical tissue of patients with cervical cancer, precancer, and noncancerous diseases. Cancer Epidemiology Biomarkers and Prevention, 7(4), 347-350.
• Denise J. Roe, . (1997). Comparison of population pharmacokinetic modeling methods using simulated data: Results from the Population Modeling Workgroup. Statistics in Medicine, 16(11), 1241-1262.
• Denise J. Roe, ., & Michael D. Karol, . (1997). Averaging pharmacokinetic parameter estimates from experimental studies: Statistical theory and application. Journal of Pharmaceutical Sciences, 86(5), 621-624.
• Hsiao-Hui Chow, ., Kristin M. Tolle, ., Denise J. Roe, ., Victor Elsberry, ., & Hsinchun Chen, . (1997). Application of neural networks to population pharmacokinetic data analysis. Journal of Pharmaceutical Sciences, 86(7), 840-845.
• Pamela J. Kling, ., Tara M. Sullivan, ., Margaret E. Leftwich, ., & Denise J. Roe, . (1997). Score for neonatal acute physiology and phlebotomy blood loss predict erythrocyte transfusions in premature infants. Archives of Pediatrics and Adolescent Medicine, 151(1), 27-31.
• Terence D. Valenzuela, ., Denise J. Roe, ., Shan Cretin, ., Daniel W. Spaite, ., & Mary P. Larsen, . (1997). Estimating effectiveness of cardiac arrest interventions: A logistic regression survival model. Circulation, 96(10), 3308-3313.
• Alan F. List, ., Catherine S. Spier, ., Thomas M. Grogan, ., Cynthia Johnson, ., Denise J. Roe, ., John P. Greer, ., Steven N. Wolff, ., Henricus J. Broxterman, ., George L. Scheffer, ., Rik J. Scheper, ., & William S. Dalton, . (1996). Overexpression of the major vault transporter protein lung-resistance protein predicts treatment outcome in acute myeloid leukemia. Blood, 87(6), 2464-2469.
• Denise J. Roe, ., Antonia M. Harford, ., Philip G. Zager, ., Thomas B. Wiltbank, ., Linda Kirlin, ., Ann Marie Della Valle, ., & David B. Van Wyck, . (1996). Iron utilization after iron dextran administration for iron deficiency in patients with dialysis-associated anemia: A prospective analysis and comparison of two agents. American Journal of Kidney Diseases, 28(6), 855-860.
• Denise J. Roe, ., David S. Alberts, ., Michael J. Wargovich, ., Roberd M. Bostick, ., Harinder S. Garewal, ., Janine Einspahr, ., Lisa Fosdick, ., Lois Ramsey, ., Cynthia Woods, ., & Daniel L. McGee, . (1996). Reproducibility of the measurement of colonic proliferation using bromodeoxyuridine across laboratories. Cancer Epidemiology Biomarkers and Prevention, 5(5), 349-353.
• Guido Tricot, ., David S. Alberts, ., Cynthia Johnson, ., Denise J. Roe, ., Robert T. Dorr, ., Dwayne Bracy, ., David H. Vesole, ., Sundar Jagannath, ., Ross Meyers, ., & Bart Barlogie, . (1996). Safety of autotransplants with high-dose melphalan in renal failure: A pharmacokinetic and toxicity study. Clinical Cancer Research, 2(6), 947-952.
• J. Einspahr, ., D. Alberts, ., T. Xie, ., J. Ritchie, ., D. Earnest, ., L. Hixson, ., M. Powell, ., D. Roe, ., & T. Grogan, . (1995). Comparison of proliferating cell nuclear antigen versus the more standard measures of rectal mucosal proliferation rates in subjects with a history of colorectal cancer and normal age-matched controls. Cancer Epidemiology Biomarkers and Prevention, 4(4), 359-366.
• Y.-M. Peng, ., Y.-S. Peng, ., Y. Lin, ., T. Moon, ., D.J. Roe, ., & C. Ritenbaugh, . (1995). Concentrations and plasma-tissue-diet relationships of carotenoids, retinoids, and tocopherols in humans. Nutrition and Cancer, 23(3), 233-246.
• D.S. Alberts, ., J. Einspahr, ., M. Aickin, ., L. Hixson, ., D. Earnest, ., D. Roe, ., & M. Powell, . (1994). Validation of proliferation indices as surrogate endpoint biomarkers. Journal of Cellular Biochemistry, 56(SUPPL. 19), 76-83.
• D.J. Roe, ., & E.L. Korn, . (1993). Time-period effects in longitudinal studies measuring average rates of change. Statistics in Medicine, 12(9), 893-900.
• C.W. Taylor, ., D.S. Alberts, ., Y.-M. Peng, ., T.M. McCloskey, ., M. Matzner, ., D.J. Roe, ., P.M. Plezia, ., G.B. Grindey, ., M. Hamilton, ., & D. Seitz, . (1992). Antitumor activity and clinical pharmacology of sulofenur in ovarian cancer. Journal of the National Cancer Institute, 84(23), 1798-1802.
• C.W. Taylor, ., W.S. Dalton, ., P.R. Parrish, ., M.C. Gleason, ., W.T. Bellamy, ., F.H. Thompson, ., D.J. Roe, ., & J.M. Trent, . (1991). Different mechanisms of decreased drug accumulation in doxorubicin and mitoxantrone resistant variants of the MCF7 human breast cancer cell line. British Journal of Cancer, 63(6), 923-929.
• D.S. Alberts, ., N. Mason-Liddil, ., P.M. Plezia, ., D.J. Roe, ., R.T. Dorr, ., R.F. Struck, ., & J.G. Phillips, . (1991). Lack of ranitidine effects on cyclophosphamide bone marrow toxicity or metabolism: A placebo-controlled clinical trial. Journal of the National Cancer Institute, 83(23), 1739-1743.
• G.J. Shamdas, ., H.S. Garewal, ., & D.J. Roe, . (1991). Usefulness of bone-marrow biopsy during induction therapy for acute myeloid leukaemia [4]. Lancet, 339(8786), 178.
• M. Lehnert, ., W.S. Dalton, ., D. Roe, ., S. Emerson, ., & S.E. Salmon, . (1991). Synergistic inhibition by verapamil and quinine of P-glycoprotein-mediated multidrug resistance in a human myeloma cell line model. Blood, 77(2), 348-354.
• S.E. Salmon, ., W.S. Dalton, ., T.M. Grogan, ., P. Plezia, ., M. Lehnert, ., D.J. Roe, ., & T.P. Miller, . (1991). Multidrug-resistant myeloma: Laboratory and clinical effects of verapamil as a chemosensitizer. Blood, 78(1), 44-50.
• M.M. Webber, ., A.S. Gomes, ., D. Roe, ., R.L. La Fontaine, ., & R.A. Hawkins, . (1990). Comparison of Biello, McNeil, and PIOPED criteria for the diagnosis of pulmonary emboli on lung scans. American Journal of Roentgenology, 154(5), 975-981.
• P. Scuderi, ., P.R. Finley, ., B.Y. Shon, ., J.N. Udall, ., D.J. Roe, ., & A.S.-F. Chong, . (1990). Regulation of tumor necrosis factor secretion in leukocytes from alpha-1-antitrypsin deficient humans. Immunological Investigations, 19(5), 453-461.
• P.M. Plezia, ., D.S. Alberts, ., J.F. Kessler, ., P.L. Berens, ., J.L. Chase, ., & D.J. Roe, . (1990). Randomized crossover comparison of high-dose intravenous metoclopramide versus a five-drug antiemetic regimen. Journal of Pain and Symptom Management, 5(2), 101-108.
• Patricia M. Plezia, ., David S. Alberts, ., John F. Kessler, ., Patricia L. Berens, ., Judy L. Chase, ., & Denise J. Roe, . (1990). Randomized crossover comparison of high-dose intravenous metoclopramide versus a five-drug antiemetic regimen. Journal of Nurse-Midwifery, 5(2), 101-108.
• C.W. Taylor, ., D.S. Alberts, ., M.A. Ketcham, ., W.G. Satterlee, ., M.T. Holdsworth, ., P.M. Plezia, ., Y.-M. Peng, ., T.M. McCloskey, ., D.J. Roe, ., M. Hamilton, ., & S.E. Salmon, . (1989). Clinical pharmacology of a novel diarylsulfonylurea anticancer agent. Journal of Clinical Oncology, 7(11), 1733-1740.
• C. Kimme-Smith, ., S.T. Cochran, ., & D.J. Roe, . (1988). Medical pattern recognition pitfalls in a clinical setting: Renal cell carcinoma survival prediction. Medical Physics, 15(3), 401-406.
• D.S. Alberts, ., E.A. Surwit, ., Y.-M. Pen, ., T. McCloskey, ., R. Rivest, ., V. Graham, ., L. McDonald, ., & D. Roe, . (1988). Phase I clinical and pharmacokinetic study of mitoxantrone given to patients by intraperitoneal administration. Cancer Research, 48(20), 5874-5877.
• F.K. Storm, ., R.M. Elashoff, ., H.W. Baker, ., E.F. Scanlon, ., B. Drury, ., M. Lee, ., D. Roe, ., & D.L. Morton, . (1988). Thermal dose-response of magnetic-induction thermoradiotherapy. Journal of Surgical Oncology, 39(2), 79-83.
• H.S. Garewal, ., E.W. Gerner, ., R.E. Sampliner, ., & D. Roe, . (1988). Ornithine decarboxylase and polyamine levels in columnar upper gastrointestinal mucosae in patients with Barrett's esophagus. Cancer Research, 48(11), 3288-3291.
• L.E. Davis, ., D.S. Alberts, ., P.M. Plezia, ., D.J. Roe, ., & D.P. Griswold, . (1988). Predictive model for plasma concentration-versus-time profiles of investigational anticancer drugs in patients. Journal of the National Cancer Institute, 80(11), 815-819.
• C. Kimme-Smith, ., L.W. Bassett, ., R.H. Gold, ., D. Roe, ., & J. Orr, . (1987). Mammographic dual-screen-dual-emulsion-film combination: Visibility of simulated microcalcifications and effect on image contrast. Radiology, 165(2), 313-318.
• F.K. Storm, ., E.F. Scanlon, ., H.W. Baker, ., D. Roe, ., & D.L. Morton, . (1987). Tumor stabilization after hyperthermia: An important criterion of response to thermal therapy. Journal of Surgical Oncology, 34(3), 143-149.
• L.A. Cagle, ., J.M. Mirra, ., F.K. Storm, ., D.J. Roe, ., & F.R. Eilber, . (1987). Histologic features relating to prognosis in synovial sarcoma. Cancer, 59(10), 1810-1814.
• R.F. Struck, ., D.S. Alberts, ., K. Horne, ., J.G. Phillips, ., Y.M. Peng, ., & D.J. Roe, . (1987). Plasma pharmacokinetics of cyclophosphamide and its cytotoxic metabolites after intravenous versus oral administration in a randomized, crossover trial. Cancer Research, 47(10), 2723-2726.
• A. Norman, ., D. Bass, ., & D. Roe, . (1985). Screening human populations for chromosome aberrations. Mutation Research, 143(3), 155-160.
• F.K. Storm, ., D.A. Christensen, ., A.J. Olch, ., A.W. Silberman, ., D.J. Roe, ., W.H. Harrison, ., R.S. Elliott, ., & D.L. Morton, . (1985). Variability of heating patterns in animals by magnetic induction hyperthermia. Journal of Surgical Oncology, 29(2), 82-88.
• F.K. Storm, ., H.W. Baker, ., E.F. Scanlon, ., H.P. Plenk, ., P.M. Meadows, ., S.C. Cohen, ., C.E. Olson, ., J.W. Thomson, ., J.D. Khandekar, ., & D. Roe, . (1985). Magnetic-induction hyperthermia. Results of a 5-year multi-institutional national cooperative trial in advanced cancer patients. Cancer, 55(11), 2677-2687.
• A. Norman, ., S. Cochran, ., D. Bass, ., & D. Roe, . (1984). Effects of age, sex and diagnostic X-rays on chromosome damage. International Journal of Radiation Biology, 46(3), 317-321.
• A.S. Gomes, ., A.R. Sinaiko, ., L. Tobian, ., J.N. Cohn, ., A.G. Formanek, ., D.J. Roe, ., & K. Amplatz, . (1984). Renal vein renin sampling in essential hypertension using hydralazine and the tourniquet test. Radiology, 153(3 I), 619-623.
• Carl A. Bertelsen, ., Armando E. Giuliano, ., David H. Kern, ., Barry D. Mann, ., Denise J. Roe, ., & Donald L. Morton, . (1984). Breast cancers: Estrogen and progesterone receptor status as a predictor of in vitro chemotherapeutic response. Journal of Surgical Research, 37(4), 257-263.
• F.Kristian Storm, ., Denise J. Roe, ., Susanne Hildebrand-Zanki, ., Gloria D. Worth, ., Donald L. Morton, ., & David H. Kern, . (1983). Natural thermal resistance of human tumor cells and the role of prostaglandin. Surgery, 94(2), 376-383.
• S.T. Cochran, ., W.S. Wong, ., & D.J. Roe, . (1983). Predicting angiography- induced acute renal function impairment: Clinical risk model. American Journal of Roentgenology, 141(5), 1027-1033.
• C. Callery, ., A.J. Cochran, ., D.J. Roe, ., W. Rees, ., S.D. Nathanson, ., J.K. Benedetti, ., R.M. Elashoff, ., & D.L. Morton, . (1982). Factors prognostic for survival in patients with malignant melanoma spread to the regional lymph nodes. Annals of Surgery, 196(1), 69-75.
• S.S. Ahn, ., R.F. Irie, ., T.H. Weisenburger, ., P.C. Jones, ., G. Juillard, ., D.J. Roe, ., & D.L. Morton, . (1982). Humoral immune response to intralymphatic immunotherapy for disseminated melanoma: Correlation with clinical response. Surgery, 92(2), 362-367.

Proceedings Publications

• Chow, H., Roe, D., Chiang, J. A., Chalasani, P., Garcia, D. O., Centuori, S. M., Tseng, H., Vedantham, S., Chow, H., Roe, D., Chiang, J. A., Chalasani, P., Garcia, D. O., Centuori, S. M., Tseng, H., & Vedantham, S. (2023). Feasibility of quantitative breast density measurements in obese women with dedicated cone-beam breast CT.. In 2023 Program of the 10th International Breast Density & Cancer Risk Assessment Workshop, a21.
• Ussery, C., Hingle, M. D., Hingle, M. D., Merchant, N. C., Going, S. B., Merchant, N. C., Stump, C. S., Roe, D., Stump, C. S., Kutob, R. M., Kutob, R. M., Kutob, R. M., Roe, D., Stump, C. S., Roe, D., Going, S. B., Merchant, N. C., Going, S. B., Ussery, C., , Ussery, C., et al. (2016, April 6). Metabolic Risk Factor Prevalence in Children Participating in a Family-Based Community Diabetes Prevention Program. In Experimental Biology Conference.
• Tate, T., Baggett, B., Rice, P., Watson, J., Orsinger, G., Nymeyer, A. C., Welge, W. A., Keenan, M., Saboda, K., Roe, D. J., Hatch, K., Chambers, S., Black, J., Utzinger, U., Barton, J., MahadevanJansen, A., VoDinh, T., & Grundfest, W. (2015, 2015). Multispectral fluorescence imaging of human ovarian and Fallopian tube tissue for early stage cancer detection. In Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII, 9313.

Presentations

• Curiel, C. N., Calvert, V., Petricoin, E., Roe, D., Saboda, K., Yozwiak, M., Krutzsch, M., & Dickinson, S. E. (2019, Spring). Increased PD-L1 expression in human skin acutely and chronically exposed to UV irradiation.. Society for Investigational Dermatology. Chicago, IL.
• Patanwala, A. E., Erstad, B. L., Roe, D., & Sakles, J. C. (2015, October). Succinylcholine is associated with increased mortality when used for rapid sequence intubation of severely head injured patients in the emergency department. ACEP Research Forum. Boston, MA.
• Hongu, N. -., Pope, B. T., Martinez, C. L., Merchant, N. C., Misner, S. L., & Roe, D. -. (2013, April). Using smartphones to maintain engagement of youth participation in 8-week walking program. Experimental Biology. Boston, MA: American Society for Nutrition.

Poster Presentations

• Chalasani, P., Roe, D., Jiralerspong, S., Ehsani Chimeh, S., Segar, J., Erdrich, J., Foster, N., Potluri, P., Farr, K., & Whittaker, M. (2022, December). Physician Practice Patterns of Breast Imaging After Treatment: Survey of Real-World Practice. San Antonio Breast Cancer Symposium. San Antonio Texas.
• Chalasani, P., Roe, D., Jiralerspong, S., Ehsani Chimeh, S., Segar, J., Erdrich, J., Foster, N., Potluri, P., Farr, K., Whittaker, M., Chalasani, P., Roe, D., Jiralerspong, S., Ehsani Chimeh, S., Segar, J., Erdrich, J., Foster, N., Potluri, P., Farr, K., & Whittaker, M. (2022, December). Physician Practice Patterns of Breast Imaging After Treatment: Survey of Real-World Practice. San Antonio Breast Cancer Symposium. San Antonio Texas.
• Chung, C., Obara, S., Saboda, K., Caulin, C., Centuori, S. M., Burtness, B., Giri, A., Steuer, C., Baker, A., Bearelly, S., Wang, S., Julian, R., Muzaffar, J., Bhatia, A., Kaczmar, J., Bauman, J., Roe, D., Saba, N., & Bauman, J. (2021, May/Spring). Randomized, phase II study of ficlatuzumab with or without cetuximab in pan-refractory, advanced head and neck squamous cell carcinoma (HNSCC).
  . ASCO.
• Dickinson, S. E., Roe, D., Saboda, K., Yozwiak, M., Krutzsch, M., & Curiel, C. N. (2019, Spring). Increased PD-L1 and CD47 expression in high-risk cutaneous squamous cell carcinomas.. Society for Investigational Dermatology Conference. Chicago, IL.
• Foote, J. A., Roe, D., Harris, R. B., Dennis, L. K., & Celaya, M. P. (2019, November). Prediction of Attrition From a Medical Weight Loss Intervention With Community Hospital Employees. Obesity Week 2019 - TOS.
• Chow, H., Algotar, A., Altbach, M. I., Galons, J., Roe, D., Thomson, C. A., Guillen-Rodriguez, J. M., Martinez, J. A., Chalasani, P., Villa Guillen, D. E., Tapia, E. O., & Trujillo, J. (2018, Fall). Abstract: Phase II clinical study of metformin for breast cancer prevention. UACC Scientific Retreat. UACC Scientific Retreat. Tucson, AZ.
• Chow, H., Algotar, A., Roe, D., Miller, J., Gallons, J. P., Altbach, M., Thomson, C. A., Chalasani, P., Cordova, C., Tapia, E., Villa-Guillen, D. E., & Trujillo, J. S. (2018, March 2018). The potential for metformin to reduce obesity-associated breast cancer risk. American Society for Preventive Oncology, 42nd Annual Conference. New York: American Society for Preventive Oncology.
• Hastings, K. T., DiCaudo, D. J., Roe, D., Cui, H., Meador, L. R., & Menon, H. (2017, May). Inducers of GILT expression in human melanoma. Society for Investigative Dermatology Annual Meeting. Portland, OR.
• Hastings, K. T., Homsi, J., Roe, D., Cui, H., Thomson, N., Menon, H., & Meador, L. R. (2017, March). Evaluating the association of GILT expression with patient outcome in metastatic melanoma. 2nd Annual Arizona Biomedical Research Commission Research Conference. Phoenix, AZ.
• Hastings, K. T., Homsi, J., Roe, D., Cui, H., Thomson, N., Menon, H., & Meador, L. R. (2017, March). Evaluating the association of GILT expression with patient outcome in metastatic melanoma. AZBio Expo in partnership with National Institue of Collaborative Healthcare. Tempe, AZ.
• Patanwala, A. E., Erstad, B. L., Roe, D., & Sakles, J. C. (2015, October). Succinylcholine is associated with increased mortality when used for rapid sequence intubation of severely head injured patients in the emergency department. American College of Emergency Physicians. Boston, MA.
• Turner, T., Kutob, R. M., Going, S. B., Merchant, N. C., Roe, D., Stump, C., & Hingle, M. D. (2014, November). E.P.I.C. Kids study: a family-focused community program to prevent type 2 diabetes in youth. The University of Arizona CALS 2014 Research Forum. University of Arizona: College of Agriculture and Life Sciences.
  More info

  The University of Arizona CALS 2014 Research Forum. E.P.I.C. Kids study: a family-focused community program to prevent type 2 diabetes in youth. T Turner* (presenting author), R Kutob, S Going, N Merchant, D Roe, C Stump, M Hingle
• Hingle, M., Going, S. B., Orr, B. J., Hongu, N. -., Merchant, N., Nichter, M. -., Roe, D. -., Borden, L., Astroth, K. A., & Marsh, S. E. (2013, August). Stealth health: Youth innovation, mobile technology, online social networking, and informal learning to promote physical activity. Society for Nutrition Education and Behavior, Annual Conference. Portland, OR.: Society for Nutrition Education and Behavior.
• Hongu, N. -., Roe, D. -., Byrd-Bredbenner, C., Worobey, J., Martin-Biggers, J., & Hernandez, G. (2013, April). HomeStyles: Shaping home environments and lifestyle practices to prevent childhood obesity: A randomized controlled trial.. Priester National Health Extension Conference, Corvallis, OR. Corvallis, OR.
• Sardo, C. L., Thomson, C. A., Hibler, E. A., Harris, R. B., Roe, D. R., Greenlee, H., Lance, M. P., Thompson, P., & Jacobs, E. T. (2013, November). Berry consumption and colorectal adenoma recurrence: a pooled analysis. American Institute for Cancer Research. Washington, DC.